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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Biological Analysis of Enzymatic Extracts from Sargassum fulvellum Using Polysaccharide Degrading Enzyme

Polysaccharide Degrading Enzyme을 이용한 참모자반 효소분해 추출물의 생리활성 연구

  • Cho, Eun Kyung (Department of Food and Nutririon, College of Medicinal Life Science, Silla University) ;
  • Kang, Su Hee (Department of Food and Nutririon, College of Medicinal Life Science, Silla University) ;
  • Choi, Young Ju (Department of Food and Nutririon, College of Medicinal Life Science, Silla University)
  • 조은경 (신라대학교 식품영양학과) ;
  • 강수희 (신라대학교 식품영양학과) ;
  • 최영주 (신라대학교 식품영양학과)
  • Received : 2013.08.07
  • Accepted : 2013.11.18
  • Published : 2013.12.30
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Abstract

SC092 strain, producing a polysaccharide degrading enzyme, was isolated from the seawater. This strain was identified as Microbulbifer sp. using the comparative sequence analysis against known 16S rRNA sequence. A polysaccharide degrading enzyme from this strain was used to acquire the enzymatic extracts of Sargassum fulvellum. DPPH radical scavenging and SOD activity of the enzyme extracts of S. fulvellum were about 61.9% and 82.9% at 2 mg/mL, respectively. Nitrite scavenging activities was 52.5% at 2 mg/mL on pH 1.2. In addition, ${\alpha}$-glucosidase inhibitory activity was also increased in a dose-dependent manner and was about 52.7% at 2 mg/mL. To determine the influence of enzyme extracts of S. fulvellum on alcohol metabolism, the generating activity of reduced-nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were measured. ADH and ALDH activities were 118.0% and 177% at 2 mg/mL, respectively. ${\alpha}$-glucosidase inhibitory activity of enzyme extracts of S. fulvellum was remarkably increased in a dose-dependent manner and was about 52.7% at 2 mg/mL. These results indicate alcoholizing and ${\alpha}$-glucosidase inhibitory activities can be enhanced by the enzymatic extracts of S. fulvellum.

Keywords

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