Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Activity of Enzymatic Extracts from Sargassum coreanum

큰잎모자반 효소적 추출물의 항산화 활성

  • Ko, Seok-Chun (Dept. of Marine Life Science, Jeju National University) ;
  • Kang, Sung-Myung (Dept. of Marine Life Science, Jeju National University) ;
  • Ahn, Gin-Nae (Dept. of Marine Life Science, Jeju National University) ;
  • Yang, Hyun-Pil (Technology Research Center, Kitto Life Co., Ltd.) ;
  • Kim, Kil-Nam (Jeju Biodiversity Research Institute (JBRI) and Jeju Hi-Tech Industry Development Institute (HiDI)) ;
  • Jeon, You-Jin (Dept. of Marine Life Science, Jeju National University)
  • 고석천 (제주대학교 해양생명과학과) ;
  • 강성명 (제주대학교 해양생명과학과) ;
  • 안긴내 (제주대학교 해양생명과학과) ;
  • 양현필 ((주)키토라이프 기술연구소) ;
  • 김길남 (제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 전유진 (제주대학교 해양생명과학과)
  • Published : 2010.04.30
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Abstract

In this study, Sargassum coreanum was enzymatically hydrolyzed to prepare water-soluble extracts by using five carbohydrates (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavozyme and Alcalase) and their potential antioxidant activity were evaluated. The Celluclast and Neutrase extracts of Sargassum coreanum exhibited better DPPH radical scavenging activities (92.42% and 92.78%, respectively) and hydrogen peroxide ($H_2O_2$) scavenging activities (58.28% and 57.97%, respectively) compared to those of other enzymatic extracts. These results suggest that Sargassum coreanum would be a good raw materials for antioxidant and enzymatic hydrolysis would be a good strategy to prepare antioxidant extracts from seaweeds.

본 연구에서는 제주 연안에 서식하는 갈조류 중 큰잎모자반의 항산화활성을 확인하기 위하여 큰잎모자반으로부터 효소적 가수분해를 이용하여 추출물을 제조한 후 이들로부터 활성산소종의 소거활성을 확인하였다. 여기서 효소적 가수분해를 이용하여 제조된 큰잎모자반의 효소적 추출물은 항산화활성이 우수하였으며, 효소적 추출물간의 유의적 차이는 없었다. 또한 단백질 분해효소 추출물에서는 Neutrase 추출물이 항산화 활성이 우수하였으며, 당 분해효소 추출물에서는 Celluclast를 이용하여 제조된 효소적 추출물이 다소 우수한 항산화효과를 나타내었다. 이와 같은 결과는 여러 가지 효소들이 복합되어진 효소가 세포벽에 있는 섬유질이나 당단백질 혹은 알긴산 고분자물질 등을 분해시키는 작용을 유도하였기 때문이라고 사료된다. 따라서 수용성의 추출물은 유기용매 추출물의 단점을 보완할 수 있을 뿐만 아니라 큰잎모자반 효소적 추출물의 생리활성물질은 식품을 포함한 다양한 분야에 응용시킬 수 있을 것으로 사료되며, 이상의 결과로 큰잎모자반은 잠재적인 기능성식품 소재로서의 가능이 충분하다고 판단된다.

Keywords

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