Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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Effect of Prunetin on TNF-${\alpha}$-Induced MUC5AC Mucin Gene Expression, Production, Degradation of $I{\kappa}B$ and Translocation of NF-${\kappa}B$ p65 in Human Airway Epithelial Cells

  • Ryu, Jiho (Department of Pharmacology, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine) ;
  • Lee, Hyun Jae (Department of Pharmacology, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine) ;
  • Park, Su Hyun (Department of Pharmacology, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine) ;
  • Sikder, Md. Asaduzzaman (Department of Pharmacology, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine) ;
  • Kim, Ju-Ock (Department of Pharmacology, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine) ;
  • Hong, Jang-Hee (Department of Pharmacology, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine) ;
  • Seok, Jeong Ho (Department of Pharmacology, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine) ;
  • Lee, Choong Jae (Department of Pharmacology, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine)
  • Received : 2013.09.11
  • Accepted : 2013.10.15
  • Published : 2013.11.30
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Abstract

Background: We investigated whether prunetin significantly affects tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$)-induced MUC5AC mucin gene expression, production, inhibitory kappa B ($I{\kappa}B$) degradation and nuclear factor kappa B (NF-kB) p65 translocation in human airway epithelial cells. Methods: Confluent NCI-H292 cells were pretreated with prunetin for 30 minutes and then stimulated with TNF-${\alpha}$ for 24 hours or the indicated periods. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The effect of prunetin on TNF-${\alpha}$-induced degradation of $I{\kappa}B$ and translocation of NF-${\kappa}B$ p65 was investigated by western blot analysis. Results: We found that incubation of NCI-H292 cells with prunetin significantly inhibited mucin production and down-regulated the MUC5AC gene expression induced by TNF-${\alpha}$. Prunetin inhibited TNF-${\alpha}$-induced degradation of $I{\kappa}B$ and translocation of NF-${\kappa}B$ p65. Conclusion: This result suggests that prunetin inhibits the NF-${\kappa}B$ signaling pathway, which may explain its role in the inhibition of MUC5AC mucin gene expression and production regulated by the NF-${\kappa}B$ signaling pathway.

Keywords

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