The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Antioxidative and Antimicrobial Activities of Water- and Ethanol-Extracts from Betula platyphylla var. japonica, Punica granatum and Rhus javanica

화피, 석류피, 염부수백피 추출물의 항산화 및 항균 효과

  • Received : 2013.04.29
  • Accepted : 2013.05.27
  • Published : 2013.05.30
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Abstract

Objectives : This study was conducted to measure the antioxidative and antimicrobial activities of water- and ethanol-extracts from Betula platyphylla var. japonica, Punica granatum and Rhus javanica against various species of anaerobic bacteria. Methods : In order to evaluate the antioxidative and antimicrobial activities of water- and ethanol-extracts, DPPH radical scavenging and superoxide dismutase (SOD)-like activities were measured in various species such as Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Propionibacterium acnes that induce skin inflammation. Also the total amount of phenol in each water- and ethanol-extract was measured to identify its role in the antioxidative and antimicrobial activities of water- and ethanol-extracts from Betula platyphylla var. japonica, Punica granatum and Rhus javanica. The antibacterial activity of the extracts was measured by confirming the lowest concentrations in disk diffusion and minimum inhibitory concentration (MIC) tests. Results : It was observed that RJE (Rhus javanica-EtOH extracts) show the highest content of total phenol. In addition, for RJE the total phenol content was higher from samples taken from domestic sources than from samples taken from foreign sources. DPPH radical scavenging activity was increased by treatment with PGE (Punica granatum-EtOH extracts), RJE and RJW (Rhus javanica-water extracts). It was observed that SOD-like activity was highest in the treatment with PGE. All of the extracts showed antimicrobial activity on S. epidermidis, S. aureus, P. acnes and E. coli, including those from Punica granatum, and it was noted that the activity was higher with RJE than with RJW. Conclusions : These results provide evidence that ethanol extracts of Punica granatum and Rhus javanica may have a beneficial role as antioxidants and antibiotics. Extracts from domestic samples of Betula platyphylla var japonica appeared to have a greater efficacy than extracts from foreign samples.

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References

  1. Ha YM, Lee BB, Bae HJ, Je GM, Kim SR, Choi JS, Choi IS. Anti-microbial Activity of Grapefruit Seed Extract and Processed Sulfur Solution against Human Skin Pathogens. J Life Sci. 2009 ; 19(1) : 94-100. https://doi.org/10.5352/JLS.2009.19.1.094
  2. Marples RR. The microflora of the face and acne lesions. J Invest Dermatol. 1974 ; 62(3) : 326-31. https://doi.org/10.1111/1523-1747.ep12724285
  3. Kim CM, Sin MK, An DK, Lee KS. Dictionary of the complete translation traditional chinese medicine. Seoul : Jeongdam. 1998 : 4950-2.
  4. Shin MK. Primaries clinical herbology. Seoul : Publisher Seoul Younglim. 1989 : 317.
  5. Shin MK. Clinical herbolog. Seoul : Namsandang. 1986 : 476-7.
  6. Department of Herbology, College of the national school. Herbology. Seoul : Yeonglimsa. 1994 : 420-1.
  7. Heo J. Eastern medicinal manual. Seoul : Corporate Cultural History. 1999 : 732, 739, 1729, 1989.
  8. Kim JC. Dictionary Chinese medicine. 1998 : 2931-4.
  9. Oh JY, Choi U, Kim YS, Shin DH. Isolation and identification of antioxidative components from Bark of Rhus javanica Linne. Korean J Food Sci Technol. 2003 ; 35(4) : 726-32.
  10. An BJ, Cho YJ, Son JH, Park JM, Lee JY, Park TS. Antioxidant effects and tyrosinase inhibition activity of extract of Prunus Sargentii Rehder. J Korean Soc Appl Bio Chem. 2006 ; 49(2) : 45-8.
  11. Yu JH, Seo CS, Yim MJ. Antimicrobial activities of Pomegranate (Punica granatum Linne) Peel Extract. J Pharmaceuitcal Sciences Sookmyung Women's Univ 2004 ; 20 : 72-6.
  12. Chung SC, Hwang BY, Oh GJ, Kang SJ. Chemical components from the stem Bark of Rhus javanica L. Kor J Pharmacogn. 1999 ; 30(3) : 295-300.
  13. Folin O, Denis W. On phosphotungasticphosphomolybdic compounds as color reagents. J Biol Chem. 1912 ; 12 : 239-49.
  14. Blois MS. Antioxidant determination by the use of a stable free radical. Nature 1958 ; 26 : 1199-200.
  15. Marklund S, Marklund G. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974 ; 47 : 468-74.
  16. Kang WH. Atlas of skin diseases. Medical-US. 2008 : 205-10.
  17. Leslie Baumann, Edmund Weisverg. Cosmetic Dermatology. Seoul : Jeongdam media. 2004 : 75-82.
  18. Ruyoung. Medical Gangmok. Beijing : Chinese medical Kojeok publisher. 1999 : 506.
  19. Daejeonmunji. Acupuncture and moxa cautery manual. Daegu : Dongyangjonghabtongsin Institute publisher. 1986 : 710-1.
  20. Choi OJ. Practical Oriental medicine. Science Encyclopedia Seojeon Publisher. 1984 : 298-99, 349-50, 502-3.
  21. Grayer RJ, Harbone JB. A survey of antifungal compounds from higer plants. Phytochem. 1994 ; 37(1) : 19-42. https://doi.org/10.1016/0031-9422(94)85005-4
  22. Lee BW, Shin DH. Screening of natural antimicrobial plant extract on food spoilage microorganisms. Korean J Food Sci Technol. 1991 ; 23(2) : 200-4.
  23. Kim BG, Rhew TH, Choe ES, Chung HY, Park KY, Rhee SH. Effect of selected persimmon leaf components against sarcoma 180 induced tumor in mice. J Korean Soc Food Nutr. 1993 ; 22(3) : 334-9.
  24. Shahidi F, Wanasundara PK. Phenolic antioxidant. Crit Rev Food Sci Nutr. 1992 ; 32 : 67-103. https://doi.org/10.1080/10408399209527581
  25. Herrman K. Occurrence and content of hydroxycinnamic and hydroxybenzoic acid compounds in foods. Crit Rev Food Sci Nutr. 1998 ; 28 : 315-47.
  26. Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka G, Nishooka I. Study on the inhibitory effects of tannins and flavonoids against the 1,1-diphenyl-2picrylhydrazyl radical. Biochemical Pharamacology. 1998 ; 56 : 213-22. https://doi.org/10.1016/S0006-2952(98)00128-2
  27. Shin JH, Lee JY, Ju JC, Lee SJ, Cho HS, Sang NJ. Chemical properties and nitrte scavenging ability of citron(Citrus junos). J Korean Soc Food Sci Nutr. 2005 ; 34(4) : 196-202. https://doi.org/10.3746/jkfn.2005.34.2.196
  28. Kuramoto T. Development and application of food materials from plant extract such as SOD. Up to date Food Processing. 1992 ; 27(3) : 22-3.
  29. Bae RN, Lee SK. Factors affecting browning and its control methods in chopped grlic. J Kor Soc Hort Sci. 1990 ; 3 : 213-8.
  30. Jo KS, Kim HK, Ha JH, Park MH, Shin HS. Flavor compounds and storage stability of essential oil from garilc distillation. Korea J Food Sci Technol. 1990 ; 22 : 50-5.
  31. Park SY, Na CS, Jeong WC, Lee JC. A Literature Study of Pericarpium Granati and Cortex Betulae Platyphyllae. J of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2012 ; 25(3) : 13-33. https://doi.org/10.6114/jkood.2012.25.3.013