Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
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CTX-M-55-Type Extended-Spectrum ${\beta}$-lactamase-Producing Shigella sonnei Isolated from a Korean Patient Who Had Travelled to China

  • Lee, Wonmok (Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine) ;
  • Chung, Hae-Sun (Department of Laboratory Medicine, Ewha Womans University School of Medicine) ;
  • Lee, Hyukmin (Department of Laboratory Medicine, Kwandong University College of Medicine) ;
  • Yum, Jong Hwa (Department of Clinical Laboratory Science, Dong-eui University) ;
  • Yong, Dongeun (Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine) ;
  • Jeong, Seok Hoon (Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine) ;
  • Lee, Kyungwon (Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine) ;
  • Chong, Yunsop (Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine)
  • Published : 2013.03.02
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Abstract

We report a case of CTX-M-55-type extended-spectrum $\beta$-lactamase (ESBL)-producing Shigella sonnei infection in a 27-year-old Korean woman who had traveled to China. The patient was admitted to the hospital due to abdominal pain, watery diarrhea, and fever ($39.3^{\circ}C$). S. sonnei was isolated from her stool specimens, and the pathogen was found to be resistant to cefotaxime due to CTX-M-55-type ESBL. Insertion sequence (IS)Ecp1 was found upstream of the $bla_{CTX-M-55}$ gene. The $bla_{CTX-M-55}$ gene was transferred from the S. sonnei isolate to an Escherichia coli J53 recipient by conjugation. Pulsed-field gel electrophoresis and Southern blotting revealed that the $bla_{CTX-M-55}$ gene was located on a plasmid of approximately 130 kb.

Keywords

References

  1. Zhang W, Luo Y, Li J, Lin L, Ma Y, Hu C, et al. Wide dissemination of multidrug-resistant Shigella isolates in China. J Antimicrob Chemother 2011;66:2527-35. https://doi.org/10.1093/jac/dkr341
  2. Christopher PR, David KV, John SM, Sankarapandian V. Antibiotic therapy for Shigella dysentery. Cochrane Database Syst Rev 2010;CD006784.
  3. Livermore DM, Mushtaq S, Nguyen T, Warner M. Strategies to overcome extended-spectrum $\beta$-lactamases (ESBLs) and AmpC $\beta$-lactamases in shigellae. Int J Antimicrob Agents 2011;37:405-9. https://doi.org/10.1016/j.ijantimicag.2010.11.028
  4. Bhattacharya D, Purushottaman SA, Bhattacharjee H, Thamizhmani R, Sudharama SD, Manimunda SP, et al. Rapid emergence of third-generation cephalosporin resistance in Shigella sp. isolated in Andaman and Nicobar Islands, India. Microb Drug Resist 2011;17:329-32. https://doi.org/10.1089/mdr.2010.0084
  5. Nguyen NT, Ha V, Tran NV, Stabler R, Pham DT, Le TM, et al. The sudden dominance of blaCTX-M harbouring plasmids in Shigella spp. Circulating in Southern Vietnam. PLoS Negl Trop Dis 2010;4:e702. https://doi.org/10.1371/journal.pntd.0000702
  6. Cheung TK, Chu YW, Tsang GK, Ngan JY, Hui IS, Kam KM. Emergence of CTX-M-type $\beta$-lactam resistance in Shigella spp. in Hong Kong. Int J Antimicrob Agents 2005;25:350-2. https://doi.org/10.1016/j.ijantimicag.2005.01.007
  7. Andres P, Petroni A, Faccone D, Pasterán F, Melano R, Rapoport M, et al. Extended-spectrum $\beta$-lactamases in Shigella flexneri from Argentina: first report of TOHO-1 outside Japan. Int J Antimicrob Agents 2005;25: 501-7. https://doi.org/10.1016/j.ijantimicag.2005.02.016
  8. Rossolini GM, D'Andrea MM, Mugnaioli C. The spread of CTX-M-type extended-spectrum $\beta$-lactamases. Clin Microbiol Infect 2008;14:33-41. https://doi.org/10.1111/j.1469-0691.2007.01867.x
  9. Hong SJ, Lee CH, Wang JH, Song W, Jung SH. Clinical characteristics of extended-spectrum $\beta$-lactamase producing Shigella sonnei infection outbreaked in Chungju Area. Korean J Lab Med 2006;26:168-73. https://doi.org/10.3343/kjlm.2006.26.3.168
  10. Kim S, Kim J, Kang Y, Park Y, Lee B. Occurrence of extended-spectrum $\beta$-lactamases in members of the genus Shigella in the Republic of Korea. J Clin Microbiol 2004;42:5264-9. https://doi.org/10.1128/JCM.42.11.5264-5269.2004
  11. Pai H, Choi EH, Lee HJ, Hong JY, Jacoby GA. Identification of CTXM-14 extended-spectrum $\beta$-lactamase in clinical isolates of Shigella sonnei, Escherichia coli, and Klebsiella pneumoniae in Korea. J Clin Microbiol 2001;39:3747-9. https://doi.org/10.1128/JCM.39.10.3747-3749.2001
  12. Lee SG, Jeong SH, Lee H, Kim CK, Lee Y, Koh E, et al. Spread of CTXM-type extended-spectrum $\beta$-lactamases among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae from a Korean hospital. Diagn Microbiol Infect Dis 2009;63:76-80. https://doi.org/10.1016/j.diagmicrobio.2008.09.002
  13. Park Y, Kang HK, Bae IK, Kim J, Kim JS, Uh Y, et al. Prevalence of the extended-spectrum $\beta$-lactamase and qnr genes in clinical isolates of Escherichia coli. Korean J Lab Med 2009;29:218-23. https://doi.org/10.3343/kjlm.2009.29.3.218
  14. Jacoby G and Bush K. $\beta$-lactamase classification and amino acid sequences for TEM, SHV and OXA extended-spectrum and inhibitor resistant enzymes. http://www.lahey.org/Studies/other.asp#table1 (Updated on Aug 2012).
  15. Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, Lee K, et al. Characterization of a new metallo-$\beta$-lactamase gene, blaNDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob Agents Chemother 2009;53:5046-54. https://doi.org/10.1128/AAC.00774-09
  16. Carattoli A, Bertini A, Villa L, Falbo V, Hopkins KL, Threlfall EJ. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods 2005;63:219-28. https://doi.org/10.1016/j.mimet.2005.03.018
  17. Bush K and Jacoby GA. Updated functional classification of $\beta$-lactamases. Antimicrob Agents Chemother 2010;54:969-76. https://doi.org/10.1128/AAC.01009-09
  18. Kiratisin P, Apisarnthanarak A, Saifon P, Laesripa C, Kitphati R, Mundy LM. The emergence of a novel ceftazidime-resistant CTX-M extendedspectrum $\beta$-lactamase, CTX-M-55, in both community-onset and hospital-acquired infections in Thailand. Diagn Microbiol Infect Dis 2007;58:349-55. https://doi.org/10.1016/j.diagmicrobio.2007.02.005
  19. Li J, Ma Y, Hu C, Jin S, Zhang Q, Ding H, et al. Dissemination of cefotaxime- M-producing Escherichia coli isolates in poultry farms, but not swine farms, in China. Foodborne Pathog Dis 2010;7:1387-92. https://doi.org/10.1089/fpd.2010.0581
  20. Sun Y, Zeng Z, Chen S, Ma J, He L, Liu Y, et al. High prevalence of blaCTX-M extended-spectrum $\beta$-lactamase genes in Escherichia coli isolates from pets and emergence of CTX-M-64 in China. Clin Microbiol Infect 2010;16:1475-81. https://doi.org/10.1111/j.1469-0691.2010.03127.x
  21. Zheng H, Zeng Z, Chen S, Liu Y, Yao Q, Deng Y, et al. Prevalence and characterisation of CTX-M $\beta$-lactamases amongst Escherichia coli isolates from healthy food animals in China. Int J Antimicrob Agents 2012;39:305-10. https://doi.org/10.1016/j.ijantimicag.2011.12.001
  22. Sjolund-Karlsson M, Howie R, Krueger A, Rickert R, Pecic G, Lupoli K, et al. CTX-M-producing non-Typhi Salmonella spp. isolated from humans, United States. Emerg Infect Dis 2011;17:97-9. https://doi.org/10.3201/eid1701.100511
  23. Tamang MD, Nam HM, Jang GC, Kim SR, Chae MH, Jung SC, et al. Molecular characterization of extended-spectrum $\beta$-lactamase and plasmid mediated AmpC $\beta$-lactamase-producing Escherichia coli isolated from stray dogs from Korea. Antimicrob Agents Chemother 2012;56:2705-12. https://doi.org/10.1128/AAC.05598-11
  24. Poirel L, Gniadkowski M, Nordmann P. Biochemical analysis of the ceftazidime-hydrolysing extended-spectrum $\beta$-lactamase CTX-M-15 and of its structurally related $\beta$-lactamase CTX-M-3. J Antimicrob Chemother 2002;50:1031-4. https://doi.org/10.1093/jac/dkf240
  25. Yu F, Chen Q, Yu X, Li Q, Ding B, Yang L, et al. High prevalence of extended- spectrum $\beta$-lactamases among Salmonella enterica Typhimurium isolates from pediatric patients with diarrhea in China. PLoS One 2011;6:e16801. https://doi.org/10.1371/journal.pone.0016801
  26. Song W, Lee H, Lee K, Jeong SH, Bae IK, Kim JS, et al. CTX-M-14 and CTX-M-15 enzymes are the dominant type of extended-spectrum $\beta$- lactamase in clinical isolates of Escherichia coli from Korea. J Med Microbiol 2009;58:261-6. https://doi.org/10.1099/jmm.0.004507-0
  27. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Twenty-second Informational supplement, M100-S22. Wanye, PA: Clinical and Laboratory Standards Institute, 2012.
  28. Eckert C, Gautier V, Arlet G. DNA sequence analysis of the genetic environment of various blaCTX-M genes. J Antimicrob Chemother 2006;57:14-23. https://doi.org/10.1093/jac/dki398
  29. Tangden T, Cars O, Melhus A, Lowdin E. Foreign travel is a major risk factor for colonization with Escherichia coli producing CTX-M-type extended- spectrum $\beta$-lactamases: a prospective study with Swedish volunteers. Antimicrob Agents Chemother 2010;54:3564-8. https://doi.org/10.1128/AAC.00220-10
  30. Tham J, Walder M, Melander E, Odenholt I. Duration of colonization with extended-spectrum $\beta$-lactamase-producing Escherichia coli in patients with travellers' diarrhoea. Scand J Infect Dis 2012;44:573-7. https://doi.org/10.3109/00365548.2011.653582

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