Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Preparation Method of Indigo Standard Solution and Variation of Indigo Contents in Blue Dye Extract from Breeding Lines of Persicaria tinctoria H. Gross

인디고 분석 표준액의 제조법 및 쪽 육성계통의 인디고 함량변이

  • Kim, Seong Ju (Department of Oriental Medicine Resources, College of Natural Science, Mokpo National University) ;
  • Ko, Jae Hyung (Department of Oriental Medicine Resources, College of Natural Science, Mokpo National University) ;
  • Park, Si Hyung (Department of Oriental Medicine Resources, College of Natural Science, Mokpo National University) ;
  • Kim, Myeong Seok (Jeonnam Agricultural Research and Extension Services) ;
  • Kim, Kwan Su (Department of Oriental Medicine Resources, College of Natural Science, Mokpo National University)
  • 김성주 (목포대학교 자연과학대학 한약자원학과) ;
  • 고재형 (목포대학교 자연과학대학 한약자원학과) ;
  • 박시형 (목포대학교 자연과학대학 한약자원학과) ;
  • 김명석 (전라남도농업기술원 쌀연구소) ;
  • 김관수 (목포대학교 자연과학대학 한약자원학과)
  • Received : 2013.04.20
  • Accepted : 2013.06.07
  • Published : 2013.06.30
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Abstract

This study was carried out to find out the optimum method of preparation of indigo standard solution and its stability, and to investigate the indigo contents in Niram, blue dye extract, from a total of 7 indigo plants and 34 breeding lines of Persicaria tinctoria H. Gross. Proper solvent for indigo standard was dimethyl sulfoxide (DMSO), and appropriate concentration was 1 mg of indigo in 10 mL of DMSO. Absorbance value of UV/Vis Spectrophotometer at 620 nm of standard solution was changed decreasingly 12 hours after the preparation of standard solution irrespective of the storage conditions such as temperature and light. Average value of absorbance of 8-fold diluted standard solutions prepared daily during 16 days was $0.210{\pm}0.005$, indicating the powder of indigo compound was stable chemically. Calibration curve was made for quantitative analysis of indigo of 7 Niram samples, and indigo contents ranged from 0.69% to 18.76% showing relatively larger variation. Across all 34 breeding lines, the range of indigo content was from 7.9 mg to 56.4 mg per 100 g of fresh leaves, averaging 25.2 mg of indigo content and showing a 47.7% coefficient of variation.

Keywords

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