Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Development of PCR Diagnosis System for Plant Quarantine Seed-borne Wheat Streak Mosaic Virus

식물검역 종자전염 Wheat Streak Mosaic Virus의 PCR 검사시스템 개발

  • Lee, Siwon (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency) ;
  • Kang, Eun-Ha (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency) ;
  • Chu, Yeon-Mee (Experiment & Analysis Division, Yeongnam Regional Office, Animal and Plant Quarantine Agency) ;
  • Shin, Yong-Gil (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency) ;
  • Ahn, Tae-Young (Department of Microbiology, Dankook University)
  • 이시원 (농림축산검역본부 식물검역기술개발센터) ;
  • 강은하 (농림축산검역본부 식물검역기술개발센터) ;
  • 추연미 (농림축산검역본부 영남지역본부 시험분석과) ;
  • 신용길 (농림축산검역본부 식물검역기술개발센터) ;
  • 안태영 (단국대학교 미생물학과)
  • Received : 2013.02.15
  • Accepted : 2013.04.29
  • Published : 2013.06.30
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Abstract

Wheat streak mosaic virus (WSMV), a member of the genus Tritimovirus in Potyviridae, severely impacts wheat and corn seed worldwide, but has yet to be detected in Korea, and hence, every effort should be made to prevent its introduction. To prevent WSMV from entering the country, it is necessary to prepare a specific, sensitive, simple, and fast detection method for routine application to plant quarantine procedures. For this reason, a two-step diagnosis system consisting of RT-PCR and nested PCR is being used for WSMV detection. In addition, a novel positive control was developed for use with the system. WSMV has been detected in seed sweet corn from Japan and seed wheat from USA by a two-step diagnosis system, the details of which are described in this study. After sequence analysis, similarities of 80.6 and 100.0% with other isolates were determined by BLAST. They showed the same topology, which was classified as 4 genotypes by various phylogenetic trees, using a poly protein encoding sequence amplification. In this analysis, WSMV-JSweet-corn2868 (JX845574) is classified as clade B, while WSMV-Uwheat1944-1 (KC754959) and WSMV-Uwheat1944-2 (KC754960) belong to clade D.

Wheat streak mosaic virus (WSMV)는 Potyviridae 과 Tritimovirus 속으로 분류되는 식물병원성 바이러스로 밀과 옥수수 종자 등에 많은 피해를 주고 있다. 본 연구에서는 관리급 식물검역 종자전염바이러스인 WSMV를 신속하고 정확하게 진단하기 위한 2단계 PCR 시스템을 개발하였으며, 실험실 오염의 여부를 확인 할 수 있는 새로운 양성 대조구를 제작하였다. 본 연구에서 개발된 PCR 시스템으로 일본에서 수입한 스위트콘 종자와 미국에서 수입한 밀 종자의 검역과정에서 WSMV-JSweet-corn2868, WSMVUwheat1944-1 및 WSMV-Uwheat1944-2를 검출하였다. 검출된 증폭 산물은 다른 WSMV의 isolates와 80.60-100.00%의 유사성을 보였다. WSMV는 계통수에서 4가지 genotype이 확인되며, 이번 연구에서 검출한 isolate WSMV-JSweet-corn2868 (JX845574)은 Clade B로 분류되었고, isolate WSMV-Uwheat1944-1 (KC754959)과 WSMV-Uwheat1944-2 (KC754960)는 Clade D로 분류되었다.

Keywords

References

  1. Animal, Plant and Fisheries Quarantine and Inspection Agency. 2013. List of Plant Quarantine Viruses in Korea in Newly Revised in 2013. Res. Plant Dis. 19, Appendix.
  2. Bandelt, H.J., Forster, P., and Rohl, A. 1999. Median-joining networks for inferring intraspecific phylogenies. Mol. Biol. Evol. 16, 37-48. https://doi.org/10.1093/oxfordjournals.molbev.a026036
  3. Caruso, P., Bertolini, E., Cambra, M., and López, M.M. 2003. A new and sensitive co-operational polymerase chain reaction for rapid detection of Ralstonia solanacearum in water. J. Microbiol. Methods 55, 257-272. https://doi.org/10.1016/S0167-7012(03)00161-1
  4. Ellis, M.H., Rebertzke, G.J., Kelman, W.M., Moore, C.S., and Hyles, J.E. 2004. Detection of Wheat streak mosaic virus in four pasture grass species in Australia. Plant Pathol. 53, 239. https://doi.org/10.1111/j.0032-0862.2004.00960.x
  5. Ellis, M.H., Rebertzke, G.J., Mago, R., and Chu, P. 2003. First report of Wheat streak mosaic virus in Australia. Australas. Plant Pathol. 32, 551-553. https://doi.org/10.1071/AP03054
  6. French, R. and Robertson, N.L. 1994. Simplified sample preparation for detection of wheat streak mosaic virus and barley yellow dwarf virus by PCR. J. Virol. Methods 49, 93-99. https://doi.org/10.1016/0166-0934(94)90059-0
  7. French, R. and Stenger, D.C. 2003. Evolution of Wheat streak mosaic virus: Dynamics of population growth within plants may explain limited variation. Annu. Rev. Phytopathol. 41, 199-214. https://doi.org/10.1146/annurev.phyto.41.052002.095559
  8. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41, 95-98.
  9. Holder, M. and Lewis, P.O. 2003. Phylogeny estimation: Traditional Raditional and Bayesian approaches. Nature Reviews 4, 275-284. https://doi.org/10.1038/nrg1044
  10. Kim, D., Hyun, J., Hwang, H., and Lee, S. 2000. RT-PCR detection of Citrus tristeza virus from early Satsuma mandarin and Yuzu Cheju island. Plant Pathol. J. 16, 48-51.
  11. Kim, Y.J., Park, S., Yie, S.W., and Kim, K.H. 2005. RT-PCR detection of dsRNA Mycoviruses infecting Pleurotus ostreatus and Agaricus blazei Murrill. Plant Pathol. J. 21, 343-348. https://doi.org/10.5423/PPJ.2005.21.4.343
  12. Lee, J.S., Cho, W.K., Lee, S.H., Choi, H.S., and Kim, K.H. 2011a. Development of RT-PCR based method for detecting five non-reported quarantine plant viruses infecting the family Cucurbitaceae or Solanaceae. Plant Pathol. J. 27, 93-97. https://doi.org/10.5423/PPJ.2011.27.1.093
  13. Lee, J.S., Cho, W.K., Choi, H.S., and Kim, K.H. 2011b. RT-PCR detection of five quarantine plant RNA viruses belonging to Potyand Tospoviruses. Plant Pathol. J. 27, 291-296. https://doi.org/10.5423/PPJ.2011.27.3.291
  14. Lee, S., Kang, E.H., Heo, N.Y., Kim, S.M., Kim, Y.J., and Shin, Y.G. 2013. Detection of Carnation necrotic fleck vrus and Carnation ringspot virus using RT-PCR. Res. Plant Dis. 19, 1-9. https://doi.org/10.5423/RPD.2013.19.1.001
  15. McKinney, H. 1937. Mosaic diseases of wheat and related cereals. US Department of Agriculture Circular. No 442.
  16. Min, J.S. 2009. Import and export for seed quarantine. J. Korea Soc. Seed Sci. Ind. 6, 8-13.
  17. Nelson, M. and McClelland, M. 1992. Use of DNA methyltransferase/endonuclease enzyme combinations for megabase mapping of chromosomes. Methods Enzymol. 216, 279-303. https://doi.org/10.1016/0076-6879(92)16027-H
  18. Pan, Y.B., Burner, D.M., and Legendre, B.L. 2000. An assessment of the phylogenetic relationship among sugarcane and related taxa based on the nucleotide sequence of 5S rRNA intergenic spacers. Genetica 108, 285-295. https://doi.org/10.1023/A:1004191625603
  19. Park, M.R. and Kim, K.H. 2004. RT-PCR detection of three non-reported fruit tree viruses useful for quarantine purpose in Korea. Plant Pathol. J. 20, 147-154. https://doi.org/10.5423/PPJ.2004.20.2.147
  20. Parker, L., Kendall, A., Berger, P.H., Shiel, P.J., and Stubbs, G. 2005. Wheat streak mosaic virus-structural parameters for a Potyvirus. Virology 340, 64-69. https://doi.org/10.1016/j.virol.2005.06.022
  21. Price, J.A., Simmons, A., Fellers, J., and Rush, C.M. 2010. Multiplex real-time RT-PCR for detection of Wheat streak mosaic virus and Triticum mosaic virus. J. Virol. Methods 165, 198-201. https://doi.org/10.1016/j.jviromet.2010.01.019
  22. Priou, S., Gutarra, L., and Aley, P. 2006. An improved enrichment broth for the sensitive detection of Ralstonia solanacearum (biovars 1 and 2A) in soil using DAS-ELISA. Plant Pathol. J. 55, 36-45. https://doi.org/10.1111/j.1365-3059.2005.01293.x
  23. Rabenstein, F., Seifers, D.L., Schubert, J., French, R., and Stenger, D.C. 2002. Phylogenetic relationships, strain diversity and biogeography of Tritimoviruses. J. Gen. Virol. 83, 895-906. https://doi.org/10.1099/0022-1317-83-4-895
  24. Rural Development Administration. 2012. Present condition and henceforward prospect of seed industry in the world. J. Korea Soc. Seed Sci. Ind. 9, 32-38.
  25. Shin, Y.G. 2009. An advanced quarantine system for the inspection of seed-borne viruses in Korea. Ph. D. thesis. Kyungpook National University, Daegu, Gyeongsangbuk-do, Korea.
  26. Stein, A., Loebenstein, G., and Koening, R. 1979. Detection of cucumber mosaic virus and bean yellow mosaic virus in gladiolus by enzyme-linked immunosobent assay (ELISA). Plant Dis. Rep. 63, 185-188.
  27. Stenger, D.C. and French, R. 2009. Wheat streak mosaic virus genotypes introduced to Argentina are closely related to isolates from the American Pacific Northwest and Australia. Arch. Virol. 154, 331-336. https://doi.org/10.1007/s00705-008-0297-1
  28. Stenger, D.C., Hall, J.S., Choi, I.R., and French, R.C. 1998. Phylogenetic relationships within the Family Potyviridae: Wheat streak mosaic virus and Brome streak mosaic virus are not members of the genus Rymovirus. Phytopathology 88, 782-787. https://doi.org/10.1094/PHYTO.1998.88.8.782
  29. Stenger, D.C., Hein, G.L., and French, R. 2006. Nested deletion analysis of Wheat streak mosaic virus HC-Pro: Mapping of domains affecting polyprotein processing and eriophyid mite transmission. Virology 350, 465-474. https://doi.org/10.1016/j.virol.2006.02.015
  30. Stenger, D.C., Seifers, D.L., and French, R. 2002. Patterns of polymorphism in Wheat streak mosaic virus: Sequence space explored by a clade of closely related viral genotypes rivals that between the most divergent strains. Virology 302, 58-70. https://doi.org/10.1006/viro.2001.1569
  31. Tatineni, S.A., Winkle, D.H.V., and French, R. 2011. The N-Terminal region of Wheat streak mosaic virus coat protein is a host- and strain-specific long-distance transport factor. J. Virol. 85, 1718-1731. https://doi.org/10.1128/JVI.02044-10
  32. Tatineni, S., Ziems, A.D., Wegulo, S.N., and French, R. 2009. Triticum mosaic virus: a distinct member of the family Potyviridae with an unusually long leader sequence. Phytopathology 99, 943-950. https://doi.org/10.1094/PHYTO-99-8-0943

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