Weed & Turfgrass Science

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Screening of Antifungal Activities of Medicinal Plants for the Control of Turfgrass Fungal Disease

잔디 병해 방제를 위한 약용식물의 항균작용 탐색

  • Kang, Jae Young (Turfgrass & Science Research Institute, Technogreen Co., Ltd.) ;
  • Kim, Dae Ho (Turfgrass & Science Research Institute, Technogreen Co., Ltd.) ;
  • Lee, Dong Gu (Department of Integrative Plant Science, Chung-Ang University) ;
  • Kim, In Seob (Turfgrass & Science Research Institute, Technogreen Co., Ltd.) ;
  • Jeon, Min Goo (Turfgrass & Science Research Institute, Technogreen Co., Ltd.) ;
  • Lee, Jae Deuk (Turfgrass & Science Research Institute, Technogreen Co., Ltd.) ;
  • Kim, Ik Hwi (Turfgrass & Science Research Institute, Technogreen Co., Ltd.) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
  • 강재영 ((주)테크노그린 잔디과학연구소) ;
  • 김대호 ((주)테크노그린 잔디과학연구소) ;
  • 이동구 (중앙대학교 식물시스템과학과) ;
  • 김인섭 ((주)테크노그린 잔디과학연구소) ;
  • 전민구 ((주)테크노그린 잔디과학연구소) ;
  • 이재득 ((주)테크노그린 잔디과학연구소) ;
  • 김익휘 ((주)테크노그린 잔디과학연구소) ;
  • 이상현 (중앙대학교 식물시스템과학과)
  • Received : 2013.02.12
  • Accepted : 2013.03.13
  • Published : 2013.03.31
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Abstract

Seven medicinal plant extracts were tested for antifungal activities against six species of the major turfgrass pathogenic fungi (Colletotrichum graminicola, Pythium spp., Rhizoctonia cerealis, Rhizoctonia solani AG1-1, Rhizoctonia solani AG2-2, and Sclerotinia homoeocarpa) using paper disk diffusion method. Three medicinal plant extracts, including Pinus densiflora showed antifungal activities. In suppression of mycelium growth test, on medium adding P. densiflora extract showed that inhibition rate of mycelium growth were above 80% in 10 mg/10 ml concentration of the extract. The inhibition rate of Pythium spp. was 100% and C. graminicola was 84.3% in 10 mg/10 ml concentrations of P. densiflora extract, respectively. In particularly, the inhibition rate of Pythium spp. was 89.5% in 2 mg/10 ml concentrations of P. densiflora extract. As a result, P. densiflora extract showed high antifungal activity to Pythium spp. and C. graminicola of the turfgrass pathogen in in vitro test.

본 연구에서는 7종의 약용식물(송절, 지구목, 측백, 대풍자, 유백피, 은행잎, 참오동나무)을 사용하여 잔디의 주요 병원균 6종(R. solani AG 2-2(IV), R. solani AG-1 IB, Pythium sp., S. homoeocarpa, R. cerealis, C. graminicola)을 대상으로 항진균 활성을 실험하였다. 디스크 확산법으로 항진균 활성이 있는 약용식물 추출물을 선발하였고, 선발된 추출물을 사용하여 균사생육 억제 효과를 검정하였다. 디스크 확산법으로 실험 한 결과 7종의 약용식물 중 송절, 측백, 유백피 등 3종의 추출물에서 항진균 활성이 있는 것으로 나타났다. 송절 추출물 1,000배 처리에서 Pythium sp.에 대하여 100%, C. graminicola에 대하여 84.3%의 균사생육 억제율을 나타내었으며, 특히 Pythium sp.에 대해서는 5,000배 처리에서도 89.5%의 균사생육 억제율을 나타내었다.

Keywords

References

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