Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Sparassis crispa Extracts on Immune Cell Activation and Tumor Growth Inhibition

꽃송이버섯(Sparassis crispa) 추출물의 면역세포 활성화 및 항암 효과

  • Kim, In-Kyu (Department of Microbiology/Research Institute of Life Science, College of Natural Sciences, Gyeongsang National University) ;
  • Yun, Young Chul (Amicogen Inc.) ;
  • Shin, Yong Chul (Amicogen Inc.) ;
  • Yoo, Jiyun (Department of Microbiology/Research Institute of Life Science, College of Natural Sciences, Gyeongsang National University)
  • 김인규 (경상대학교 자연과학대학 미생물학과/생명과학연구원) ;
  • 윤영철 (아미코젠(주)) ;
  • 신용철 (아미코젠(주)) ;
  • 유지윤 (경상대학교 자연과학대학 미생물학과/생명과학연구원)
  • Received : 2013.05.20
  • Accepted : 2013.08.08
  • Published : 2013.08.30
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Abstract

Sparassis crispa is an edible mushroom with medicinal properties that contains more than 40% ${\beta}$-glucan. The role of S. crispa in regulating the functional activation of macrophages has yet to be fully elucidated. The objective of this study was to investigate the molecular mechanism underlying the immune-stimulatory function of S. crispa soluble ${\beta}$-glucan and extracts on macrophages. In this study, we showed that S. crispa soluble ${\beta}$-glucan was able to stimulate TNF-${\alpha}$ and IL-$1{\beta}$ production through NF-${\kappa}B$ activation in Raw 264.7 cells. We also showed that S. crispa extracts could not only enhance TNF-${\alpha}$ production in Raw 264.7 cells, but also suppress tumor growth in vivo. All of our results suggest that S. crispa could be developed as a promising immunostimulatory principle, applicable to cancer patients.

꽃송이버섯(Sparassis crispa)은 항암 및 면역증강 효과가 뛰어나다고 알려져 있는 베타-글루칸의 함량이 건조중량의 40% 이상인 것으로 보고되고 있는 산림버섯이다. 일반적으로 버섯에서 추출한 베타-글루칸의 면역활성 및 항암 효과는 널리 알려져 있는 반면 꽃송이버섯 유래의 베타-글루칸에 의한 대식세포의 활성화 기작 및 자실체 추출물에 의한 항암 효과는 아직 보고되지 않았다. 본 연구에서는 꽃송이버섯 유래 베타-글루칸의 면역활성 효과를 확인하고자 마우스의 대식세포인 Raw 264.7 cell을 이용하였으며, 베타-글루칸 처리시 TNF-${\alpha}$와 interleukin-$1{\beta}$(IL-$1{\beta}$)와 같은 사이토카인의 발현 및 분비가 증가됨을 확인하였고, 이러한 과정에 필수적인 역할을 담당하는 전사 인자인 NF-${\kappa}B$가 활성화됨도 확인하였다. 뿐만 아니라 꽃송이버섯 추출물 처리 시 TNF-${\alpha}$의 분비가 증가됨과 함께 in vivo에서 암의 성장을 억제하는 기능이 있음을 확인하였다. 이러한 결과들은 꽃송이버섯이 면역활성을 증가시켜 암의 성장을 억제하는 건강 기능성 식품으로서의 개발 가능성이 높음을 의미하는 것이다.

Keywords

References

  1. Bano, Z. and Rajarathnam, S. 1988. Pleurotus mushrooms. Part II. Chemical composition, nutritional value, post-harvest physiology, preservation, and role as human food. Crit Rev Food Sci Nutr 27, 87-158. https://doi.org/10.1080/10408398809527480
  2. Choi, S. J., Lee, Y. S., Kim, J. K., Kim, J. K. and Lim, S. D. 2010. Physiological activation of extract from edible mushrooms. J Korean Soc Food Sci Nutr 39, 1087-1096. https://doi.org/10.3746/jkfn.2010.39.8.1087
  3. Choi, W. S., Shin, P. G., Yoo, Y. B., Noh, H. J. and Kim, G. D. 2013. Anti-inflammatory effects of Sparassis crispa extracts. J Mushroom Sci Production 11, 46-51.
  4. Food Code. 2003. Conduct Laboratory Testing According to Specifications and Test Methods of the Food Code. Pp. 894-918, Korea Food & Drug Administration, Moon Yong Press, Seoul.
  5. Fukuda, K., Uematsu, T., Hamada, A. and Akiya, S. 1975. The polysaccharide from Lampteromyces Japonicus. Chem Pharm Bull 23, 1955-1961. https://doi.org/10.1248/cpb.23.1955
  6. Harada, T., Miura, N. N., Adachi, Y., Nakajima, M., Yadomae, T. and Ohno, N. 2002. IFN-$\gamma$ induction by SCG, 1,3-$\beta$-D-glucan from Sparassis crispa, in DBA/2 mice in vitro. J Interferon Cytokine Res 22, 1227-1239. https://doi.org/10.1089/10799900260475759
  7. Hong, J. S., Kim, Y. H., Kim, M. K., Kim, Y. S. and Sohn, H. S. 1989. Contents of free amino acids and total amino acids in Agaricus bisporus, Pleurotus ostreatus and Lentinus edodes. Korean J Food Sci Technol 21, 58-62.
  8. Kim, M. Y., Seguin, P., Ahn, J. K., Kim, J. J., Chun, S. C., Kim, E. H., Seo, S. H., Kang, E. Y., Kim, S. L. and Park, Y. J. 2008. Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. J Agr Food Chem 56, 7265-7270. https://doi.org/10.1021/jf8008553
  9. Kirk, P. M., Cannon, P. F., David, J. C. and Stalpers, J. A. 2001. Ainsworth & Bisby's Dictionary of the Fungi. Ninth edition. CABI Bioscience.
  10. Lee, S. K., Yoo, Y. J. and Kim, C. S. 1994. Studies on the chemical components in Ganoderma lucidum. Korean J Food Sci Technol 21, 890-894.
  11. Lee, S. Y., Lee, Y. G., Byeon, S. E., Han, S., Choi, S. S., Kim, A. R., Lee, J., Lee, S. J., Hong, S. and Cho, J. Y. 2010. Mitogen activated protein kinases are prime signalling enzymes in nitric oxide production induced by soluble β-glucan from Sparassis crispa. Arch Pharm Res 33, 1753-1760. https://doi.org/10.1007/s12272-010-1107-3
  12. Lee, Y. S., Kim, J. B., Shin, S. R. and Kim, N. W. 2006. Analysis of nutritional components of Lepista nuda. Korean J Food Preserv 13, 375-381.
  13. Oh, D. S., Park, J. M., Park, H., Ka, K. H. and Chun, W. J. 2009. Site characteristics and vegetation structure of the habitat of cauliflower mushroom (Sparassis crispa). Korean J Mycol 37, 33-40. https://doi.org/10.4489/KJM.2009.37.1.033
  14. Ohno, N., Miura, N. N., Nakajima, M. and Yadomae, T. 2000. Antitumor 1,3-beta-glucan from cultured fruit body of Sparassis crispa. Biol Pharm Bull 23, 866-872. https://doi.org/10.1248/bpb.23.866
  15. Park, H. G., Shim, Y. Y., Choi, S. O. and Park, W. M. 2009. New method development for nanoparticle extraction of water-soluble beta-(1-3)-D-glucan from edible mushrooms, Sparassis crispa and Phellinus linteus. J Agric Food Chem 57, 2147-2154. https://doi.org/10.1021/jf802940x
  16. Raja, B. 2011. Veratric acid, a phenolic acid attenuates blood pressure and oxidative stress in L-NAME induced hypertensive rats. Eur J Pharmacol 671, 87-94. https://doi.org/10.1016/j.ejphar.2011.08.052
  17. Shim, S. M., Im, K. H., Lee, W. Y., Kim, J. W., Shim, M. J., Lee, M. W. and Lee, T. S. 2003. Studies on immune-modulatory and antitumor effects of crude polysaccharides extracted from Paecilomyces sincluirii. Korean J Mycol 31, 155-160. https://doi.org/10.4489/KJM.2003.31.3.155
  18. Shin, H. J., Oh, D. S., Lee, H. D., Kang, H. B., Lee, C. W. and Cha, W. S. 2007. Analysis of mineral, amino acid and vitamin contents of fruiting body of Sparassis crispa. J Life Sci 17, 1290-1293. https://doi.org/10.5352/JLS.2007.17.9.1290
  19. Shon, M. Y., Seo, K. I., Choi, S. Y., Sung, N. J., Lee, S. W. and Park, S. K. 2006. Chemical compounds and biological activity of Phellinus baumii. J Korean Soc Food Sci Nutr 35, 524-529. https://doi.org/10.3746/jkfn.2006.35.5.524
  20. Soda, K., Tanaka, H. and Esaki, N. 1983. Amino Acids, Biotechnology. In Rehm, H. J. and Reed, G. (eds.), Vol. 3, pp. 479, Verlag Chemie, D-6940 Weinheim, Germany.
  21. Suzuki, M., Arika, T., Ameriya, T. and Fujiwara, M. 1982. Cooperative role of T lymphocytes and macrophages in antitumor activity of mice pretreated with schizophyllan. Jpn J Exp Med 50, 59-65.
  22. Yamamoto, K., Kimura, T., Sugitachi, A. and Matsuura, N. 2009. Anti-angiogenic and anti-metastatic effect of $\beta$-1,3- D-glucan purified from hanabiratake, Sparassis crispa. Biol Pharm Bull 32, 259-263. https://doi.org/10.1248/bpb.32.259

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