The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Isolation and Purification of Antibacterial Components in Cortex Phellodendri

황백나무로부터 항균성분의 분리 및 정제

  • Kim, Jung-Bae (Dept. of Food Nutrition and Cookery, Sangji Youngseo College) ;
  • Shin, Woon-Seob (Dept. of Microbiology, Kwandong University College of Medicine) ;
  • Kwon, Young-In (Dept. of Food and Nutrition, Hannam University) ;
  • Bang, Byung-Ho (Dept. of Food and Nutrition Science, Eulji University)
  • 김중배 (상지영서대학교 식품영양조리학과) ;
  • 신운섭 (관동대학교 의과대학 미생물학교실) ;
  • 권영인 (한남대학교 식품영양학과) ;
  • 방병호 (을지대학교 식품영양학과)
  • Received : 2013.08.01
  • Accepted : 2013.09.10
  • Published : 2013.09.30
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Abstract

Cortex Phellodendri (CP) is derived from the dried bark of Phellodendron amurense. It has been widely used as a drug in traditional Korea medicine for treating diarrhea, jaundice, swelling pains in the knees and feet, urinary tract infections and infections of the body surface. In this study, preparative centrifugal partition chromatography (CPC) was successfully carried out to separate antibacterial compounds from a CP methanol extract. The optimum two-phase CPC solvent system was composed of n-butanol: acetic acid: water (4:1:5 v/v/v). The flow rate of the mobile phase was 3 $m{\ell}/min$ in ascending mode with rotation at 1,000 rpm. The CPC-separated fraction and purification procedures were carried out by preparatory HPLC. Palmatine weas identified by $^1H$, $^{13}C$-nuclear magnetic resonance and electrospray ionization-mass spectroscopy spectral data analysis.

황백 껍질은 황벽나무(Phellodendron amurense)의 건조된 수피로부터 얻어진다. 이 수피는 한국의 전통 한약제로서, 설사, 황달, 무릎과 발의 통증, 요도관 및 피부 감염증에 폭넓게 사용되어 왔다. 본 연구는 황벽나무의 메탄올 추출액으로부터 항균성 화합물 분리를 위해 CPC 방법으로 효과적으로 수행하였다. 두 용매의 CPC 최적조성은 n-butanol:acetic acid:water(4:1:5 v/v/v)이었다. 이동상의 유속은 1,000 rpm 회전력에서 상승법으로 분당 3 $m{\ell}$ 속도로 전개시켰다. CPC에서 분리된 분획분은 prep-HPLC로 정제하였다. 분리된 palmatine은 $^1H$, $^{13}C$-NMR, ESI-MS 데이터 분석으로 확인하였다.

Keywords

References

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