Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Cordycepin on the Expression of the Inflammatory Cytokines TNF-alpha, IL-6, and IL-17A in C57BL/6 Mice

  • Seo, Min Jeong (Department of Biotechnology, Dong-A University) ;
  • Kim, Min Jeong (Department of Biotechnology, Dong-A University) ;
  • Lee, Hye Hyeon (Department of Biotechnology, Dong-A University) ;
  • Park, Jeong Uck (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Kang, Byoung Won (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Kim, Gi-Young (Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University) ;
  • Rhu, En Ju (Department of Cosmetology, Hanseo University) ;
  • Kim, Jung-In (School of Food & Life Sciences, Inje University) ;
  • Kim, Kwang Hyuk (Department of Microbiology, Kosin University College of Medicine) ;
  • Jeong, Yong Kee (Department of Biotechnology, Dong-A University)
  • Received : 2012.11.14
  • Accepted : 2012.12.01
  • Published : 2013.02.28
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Abstract

Culture supernatants of splenocytes from C57BL/6 mice were exposed to 0.3, 1.0, and 3.0 ${\mu}g/ml$ cordycepin plus 3.0 ${\mu}g/ml$ lipopolysaccharide (LPS) to investigate the effects of cordycepin (3'-deoxyadenosine) on the production of inflammatory cytokines. Coadministration of 3.0 ${\mu}g/ml$ cordycepin with LPS in cultured murine spleen cells significantly diminished expression of the inflammatory cytokines tumor necrosis factor-${\alpha}$ and interleukin-6 (IL-6) in a time-dependent manner. Expression of the inflammatory cytokine IL-17A was substantially downregulated in a timeand concentration-dependent manner at all cordycepin concentrations. These findings suggest that cordycepin downregulates the immediate hypersensitivity reaction stimulated by LPS.

Keywords

References

  1. Arican, O., M. Aral, S. Sasmaz, and P. Ciragil. 2005. Serum levels of TNF-$\alpha$, IFN-gamma, IL-6, IL-8, IL-12, IL-17, and IL-18 in patients with active psoriasis and correlation with disease severity. Mediators Inflamm. 24: 273.
  2. Bhagat, K. and P. Vallance. 1997. Inflammatory cytokines impair endothelium-dependent dilatation in human veins in vivo. Circulation 96: 3042-3047. https://doi.org/10.1161/01.CIR.96.9.3042
  3. Bok, J. W., L. Lermer, J. Chilton, H. G. Klingeman, and G. H. Towers. 1999. Antitumor sterols from the mycelia of Cordyceps sinensis. Phytochemistry 51: 891-898. https://doi.org/10.1016/S0031-9422(99)00128-4
  4. Boguniewicz, M., R. J. Martin, D. Martin, U. Gibson, A. Celniker, M. Williams, and Y. M. Donald. 1995. The effects of nebulized recombinant interferon-gamma in asthmatic airways. J. Allergy Clin. Immunol. 95: 133-135. https://doi.org/10.1016/S0091-6749(95)70162-1
  5. Borish, L. C. and J. W. Steinke. 2003. Cytokines and chemokines. J. Allergy Clin. Immunol. 111: S460-S475. https://doi.org/10.1067/mai.2003.108
  6. Cho, M. A., D. S. Lee, M. J. Kim, J. M. Sung, and S. S. Ham. 2003. Antimutagenicity and cytotoxicity of cordycepin isolated from Cordyceps militaris. Food Sci. Biotechnol. 12: 472-475.
  7. Choi, S. B., C. H. Park, M. K. Choi, D. W. Jun, and S. M. Park. 2004. Improvement of insulin resistance and insulin secretion by water extracts of Cordyceps militaris, Phellinus linteus, and Paecilomyces tenuipes in 90% pancreatectomized rats. Biosci. Biotechnol. Biochem. 68: 2257-2264. https://doi.org/10.1271/bbb.68.2257
  8. Cunningham, K. G., W. Manson, F. S. Spring, and S. A. Hutchinson. 1950. Cordycepin, a metabolic product isolated from cultures of Cordyceps militaris (Linn.). Nature 166: 949.
  9. Edrees, A. F., S. N. Misra, and N. I. Abdou. 2005. Anti-tumor necrosis factor (TNF) therapy in rheumatoid arthritis: Correlation of TNF-alpha serum level with clinical response and benefit from changing dose or frequency of infliximab infusions. Clin. Exp. Rheumatol. 23: 469.
  10. Ito, H. 2003. IL-6 and Crohn's disease. Curr. Drug Targets Inflamm. Allergy 2: 125.
  11. Jeong, M. H., M. J. Seo, J. U. Park, B. W. Kang, K. S. Kim, J. Y. Lee, et al. 2012. Effect of cordycepin purified from Cordyceps militaris on Th1 and Th2 cytokines in mouse splenocytes. J. Microbiol. Biotechnol. 22: 1161-1164. https://doi.org/10.4014/jmb.1203.03039
  12. Kodama, E. N., R. P. McCaffrey, K. Yusa, and H. Mitsuya. 2000. Antileukemic activity and mechanism of action of cordycepin against terminal deoxynucleotidyl transferase-positive (TdT+) leukemic cells. Biochem. Pharmacol. 59: 273-281. https://doi.org/10.1016/S0006-2952(99)00325-1
  13. Lack, G., H. S. Nelson, D. Amran, A. Oshiba, T. Jung, K. L. Bradley, et al. 1997. Rush immunotherapy results in allergenspecific alterations in lymphocyte function and interferon-gamma production in CD4+ T cells. J. Allergy Clin. Immunol. 99: 530-538. https://doi.org/10.1016/S0091-6749(97)70081-2
  14. Li, S. P., P. Li, C. M. Lai, Y. X. Gong, K. W. Kan, T. X. Dong, et al. 2004. Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography. J. Chromatgr. A 1036: 239-243. https://doi.org/10.1016/j.chroma.2004.02.080
  15. Lundberg, I., A. K. Ulfgren, P. Nyberg, U. Andersson, and L. Klareskog. 1997. Cytokine production in muscle tissue of patients with idiopathic inflammatory myopathies. Arthritis Rheum. 40: 865-874. https://doi.org/10.1002/art.1780400514
  16. Müller, W. E., B. E. Weiler, R. Charubala, W. Pfleiderer, L. Leserman, R. W. Sobol, et al. 1991. Cordycepin analogues of 2', 5'-oligoadenylate inhibit human immunodeficiency virus infection via inhibition of reverse transcriptase. Biochemistry 30: 2027- 2033. https://doi.org/10.1021/bi00222a004
  17. Naka, T., N. Nishimoto, and T. Kishimoto. 2002. The paradigm of IL-6: From basic science to medicine. Arthritis Res. 4: S233-S242. https://doi.org/10.1186/ar565
  18. Nakamura, K., K. Konoha, N. Yoshikawa, Y. Yamaguch, S. Kagota, K. Shinozuka, and M. Kunitomo. 2005. Effect of cordycepin (3'-deoxyadenosine) on hematogenic lung metastatic model mice. In Vivo 19: 137-141.
  19. Nakamura, K., N. Yoshikawa, Y. Yamaguchi, S. Kagota, K. Shinozuka, and M. Kunitomo. 2006. Antitumor effect of cordycepin (3'-deoxyadenosine) on mouse melanoma and lung carcinoma cells involves adenosine A3 receptor stimulation. Anticancer Res. 26: 43-48.
  20. Old, L. J. 1985. Tumor necrosis factor (TNF). Science 230: 630-632. https://doi.org/10.1126/science.2413547
  21. Osuchowski, M. F., K. Welch, J. Siddiqui, and D. G. Remick. 2006. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol. 177: 1967-1974. https://doi.org/10.4049/jimmunol.177.3.1967
  22. Rook, A. H., H. Masur, H. C. Lane, W. Frederick, T. Kasahara, A. M. Macher, et al. 1983. Interleukin-2 enhances the depressed natural killer and CMV specific cytotoxic activation of lymphocytes from patients with the acquired immunodeficiency syndrome. J. Clin. Invest. 72: 398-403. https://doi.org/10.1172/JCI110981
  23. Ruffolo, C., M. Scarpa, D. Faggian, A. Pozza, F. Navaglia, R. D'Inca, et al. 2008. Cytokine network in rectal mucosa in perianal Crohn's disease: Relations with inflammatory parameters and need for surgery. Inflamm. Bowel Dis. 14: 1406. https://doi.org/10.1002/ibd.20486
  24. Schoenborn, J. R. and C. B. Wilson. 2007. Regulation of interferon-gamma during innate and adaptive immune responses. Adv. Immunol. 96: 41-101. https://doi.org/10.1016/S0065-2776(07)96002-2
  25. Smith, K. A., L. B. Lachman, J. J. Oppenheim, and M. F. Favata. 1980. The functional relationship of the interleukins. J. Exp. Med. 151: 1551-1556. https://doi.org/10.1084/jem.151.6.1551
  26. Smolen, J. S. and R. N. Maini. 2006. Interleukin-6: A new therapeutic target. Arthritis Res. Ther. 8 (Suppl 2): S5. https://doi.org/10.1186/ar1969
  27. Sugar, A. M. and R. P. McCaffrey. 1998. Antifungal activity of 3-deoxyadenosine (cordycepin). Antimicrob. Agents Chemother. 42: 1424-1427.
  28. Thomadaki, H., A. Scorilas, C. M. Tsiapalis, and M. Havredaki. 2008. The role of cordycepin in cancer treatment via induction or inhibition of apoptosis: Implication of polyadenylation in a cell type specific manner. Cancer Chemother. Pharmacol. 61: 251-265.
  29. Yu, R., W. Yang, L. Song, C. Yan, Z. Zhang, and Y. Zhao. 2007. Structural characterization and antioxidant activity of a polysaccharide from the fruiting bodies of cultured Cordyceps militaris. Carbohydr. Polym. 70: 430-436. https://doi.org/10.1016/j.carbpol.2007.05.005
  30. Yun, Y. H., S. H. Han, S. J. Lee, S. K. Ko, C. K. Lee, N. J. Ha, and K. J. Kim. 2003. Anti-diabetic effects of CCCA, cmESS, and cordycepin from Cordyceps militaris and the immune responses in streptozotocin-induced diabetic mice. Nat. Prod. Sci. 9: 291-298.
  31. Yoo, H. S., J. W. Shin, J. H. Cho, C. G. Son, Y. W. Lee, S. Y. Park, and C. K. Cho. 2004. Effect of Cordyceps militaris extract on angiogenesis and tumor growth. Acta Pharmacol. Sin. 25: 657-665.
  32. Zhou, X., C. U. Meyer, P. Schmidtke, and F. Zepp. 2002. Effect of cordycepin on interleukin-10 production of human peripheral blood mononuclear cells. Eur. J. Pharmacol. 453: 309-317. https://doi.org/10.1016/S0014-2999(02)02359-2

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