Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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A comparison of antioxidant activity of Korean White and Red Ginsengs on H2O2-induced oxidative stress in HepG2 hepatoma cells

  • Sohn, Sang-Hyun (Department of Medicinal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Si-Kwan (Department of Biomedical Chemistry, College of Biomedical & Health Science, Konkuk University) ;
  • Kim, Young-Ock (Department of Medicinal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Hyung-Don (Department of Medicinal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Shin, Yu-Su (Department of Medicinal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Yang, Seung-Ok (Department of Medicinal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Seung-Yu (Department of Medicinal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Sang-Won (Department of Medicinal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • Received : 2013.01.08
  • Accepted : 2013.07.18
  • Published : 2013.10.15
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Abstract

The aim of this study was to determine and compare the preventive effect of Korean White Ginseng and Red Ginseng on oxidative stress in $H_2O_2$-treated HepG2 cells. The roots of ginseng were extracted with 70% methanol and partitioned with butanol to obtain saponin fractions, which have been known as bioactive constituents of ginseng. 2',7'-Dichlorofluorescein diacetate (DCF-DA) assay and malondialdehyde (MDA) content were measured for evaluating intracellular reactive oxygen species (ROS) generation. Also, mRNA expressions and activities of antioxidant enzymes were analyzed to determine the antioxidant activity of saponin or non-saponin fractions of ginsengs. According to DCF-DA assay, $H_2O_2$-induced MDA release and ROS generation were significantly reduced by treatment with saponin fractions of white and red ginseng roots. Also, saponin fractions increased effectively intracellular antioxidant enzyme activities including catalase, glutathione peroxidase and superoxide dismutase in $H_2O_2$-treated HepG2 hepatoma cells. In general, red ginseng was more effective than white ginseng for reducing oxidative stress. These results indicate that administration of red ginseng may certainly contribute relatively stronger than white ginseng to prevent from damaging liver function by oxidative stress.

Keywords

References

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