Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Identification of Toxin Gene and Antibiotic Resistance of Staphylococcus Aureus Isolated from Agricultural Product Cultivation Environments

농산물 생산 환경에서 분리된 Staphylococcus aureus의 항생제 내성 및 독소 유전자 확인

  • Park, Su-Hee (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Kim, Jeong-Sook (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Kyeong-Yeol (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Chung, Duck-Hwa (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Shim, Won-Bo (School of Physics and Chemistry, Gwangju Institute of Science and Technology)
  • 박수희 (경상대학교 응용생명과학부) ;
  • 김정숙 (경상대학교 농업생명과학연구원) ;
  • 김경열 (경상대학교 응용생명과학부) ;
  • 정덕화 (경상대학교 응용생명과학부) ;
  • 심원보 (광주과학기술원 물리화학부)
  • Received : 2013.08.19
  • Accepted : 2013.09.25
  • Published : 2013.10.31
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Abstract

Objectives: This study was undertaken to analyze Staphylococcus aureus from cultivation environments for agricultural products and to confirm antibiotic resistance and enterotoxin genes for the isolated S. aureus. Methods: A total of 648 samples were collected from apple, peach, ginseng and balloon flower farms. S. aureus was isolated from soil, agricultural water, personal hygiene elements (hands, gloves and clothes) and work utensils (boxes). Results: S. aureus was detected in a total of 25 samples and 72 strains were isolated. The resistance rate of the isolated S. aureus strains was confirmed at 33.3%, with 24 resistant strains among the total of 72. Fourteen different patterns types were found, and three pattern types (NV, OX, VA) were confirmed most frequently. As result of the detection of enterotoxin gene type, four gene types (sea: 1, sed: 4, seg: all isolated S. aureus, sei: all isolated S. aureus) were analyzed among a total of nine types. Conclusions: This study demonstrates that personal hygiene techniques should be properly managed, such as washing and sterilization before or after work, because agricultural contamination by S. aureus frequently developed through improper management.

Keywords

References

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