The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Antioxidant Activity and Protective Effects of Anthocyanins-Rich Fraction from Korean Purple Sweet Potato Variety, "Shinjami" against Oxidative Stress in HepG2 Cell

자색고구마 신자미로부터 분리한 안토시아닌 분획물의 항산화 활성과 산화스트레스에 대한 간세포 보호 효과

  • Jang, Hwan Hee (Functional Food & Nutrition Division, Department of Agro-Food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Nam, Song Yee (Functional Food & Nutrition Division, Department of Agro-Food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Mi Ju (Functional Food & Nutrition Division, Department of Agro-Food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jung Bong (Functional Food & Nutrition Division, Department of Agro-Food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Haeng Ran (Functional Food & Nutrition Division, Department of Agro-Food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Young Min (Functional Food & Nutrition Division, Department of Agro-Food Resources, National Academy of Agricultural Science, Rural Development Administration)
  • 장환희 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 남송이 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 김미주 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 김정봉 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 김행란 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 이영민 (농촌진흥청 국립농업과학원 기능성식품과)
  • Received : 2014.11.05
  • Accepted : 2014.11.14
  • Published : 2014.12.31
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Abstract

Anthocyanins, a class of flavonoids, are natural water-soluble pigments, which are mainly found in vegetables and fruits. Anthocyanins have attractive pharmacological activities, such as anti-oxidant, anti-inflammatory, anti-cancer, and anti-diabetic. The purpose of this study was to investigate the protective effects of anthocyanins-rich fraction (ANF) from Korean purple sweet potato variety, "Shinjami", against hydrogen peroxide ($H_2O_2$)-induced oxidative stress. In our results, pre-treatment of HepG2 cells with ANF ($10{\mu}g/mL$) significantly prevented cell death and maintained cell integrity, following exposure to 0.9 mM hydrogen peroxide. The $H_2O_2$-dependent production of intracellular ROS was also significantly decreased by pre-treatment with ANF (6 h, $10{\mu}g/mL$). In addition, ANF increased the mRNA levels of antioxidant enzymes, catalase and glutathione level in $H_2O_2$-treated HepG2 cells. These results indicated that ANF protected HepG2 cells against $H_2O_2$-induced oxidative stress by inducing protective system.

Keywords

References

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