Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Ameliorating Effects of Lactic Acid-fermented Garlic Extracts on Oleic Acid-induced Hepatic Steatosis

유산균 발효 마늘 추출물의 oleic acid로 유도된 비알코올성 지방간에 대한 개선 효과

  • 이희섭 (고려대학교 식품생명공학과) ;
  • 임원철 (고려대학교 식품생명공학과) ;
  • 최지휘 ((주)바이오랜드 생명공학연구소) ;
  • 유희종 ((주)바이오랜드 생명공학연구소) ;
  • 김기호 ((주)바이오랜드 생명공학연구소) ;
  • 이승현 ((주)바이오랜드 생명공학연구소) ;
  • 조홍연 (고려대학교 식품생명공학과)
  • Received : 2014.09.25
  • Accepted : 2014.10.30
  • Published : 2014.12.31
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Abstract

In this study, the ameliorating effects of lactic acid-fermented garlic extract (LAFGE) on non-alcoholic fatty liver were investigated using oleic acid-induced steatotic HepG2 cells. The ameliorating mechanism was analyzed by RT-PCR and Western blot. Treatment with 1 mg/mL LAFGE decreased intracellular lipid accumulation approximately 1.5-fold, compared to that achieved with non-fermented garlic extract. LAFGE reduced fatty acid influx into hepatocytes through down-regulation of FAT/CD36 mRNA expression in the steatotic HepG2 cells. $PPAR{\alpha}$ and CPT-1 mRNA expression was significantly up-regulated by LAFGE treatment of HepG2 cells as a consequence of activation of beta oxidation. Additionally, the treatment with 1 mg/mL LAFGE highly down-regulated mRNA expression of SREBP-1c and FAS to 51% and 35%, respectively. LAFGE showed concentration-dependent down-regulation patterns in protein expression of SREBP-1c and FAS, as determined by Western blot. These results suggest that LAFGE treatment improves hepatic steatosis triggered by the imbalance of hepatic lipid metabolism owing to oleic acid treatment.

본 연구에서는 oleic acid로 유도시킨 비알코올성 지방간 HepG2 세포 모델을 활용하여 LAFGE의 비알코올성 지방간 개선 효과를 검토하고 RT-PCR과 Western blot으로 메카니즘을 해석하였다. LAFGE는 1 mg/mL 농도에서 NFGE보다 세포 내 지방축적을 약 1.5배 저해하였다. Fig. 5에 나타낸 바와 같이 LAFGE는 FAT/CD36 mRNA 발현을 감소시켜 세포 내로 유입되는 지방산의 양을 감소시켰으며, $PPAR{\alpha}$와 CPT-1의 mRNA 발현을 증강시킴으로써 지방산의 베타-산화를 촉진시켰다. 또한 LAFGE는 지방합성을 촉진하는 전사인자인 SREBP-1c와 그의 조절을 받는 FAS의 mRNA 발현 수준을 oleic acid 처리시보다 각각 51%와 35%까지 크게 감소시켰다. 뿐만 아니라 LAFGE는 농도의존적으로 SREBP-1c와 FAS의 단백질 발현도 감소시켰다. 이 결과들은 LAFGE가 oleic acid처리에 의해 유도된 HepG2 세포 내의 지방축적을 개선할 수 있음을 시사하였다.

Keywords

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