Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Characterization of Biogenic Amine-reducing Pediococcus pentosaceus Isolated from Traditionally Fermented Soybean Products

전통 장류에서 분리한 Biogenic Amines 저감 유산균 Pediococcus pentosaceus의 분리 및 특성

  • Oh, HyeonHwa (Department of Biological Sciences, Chonbuk National University) ;
  • Ryu, MyeongSeon (Department of Biological Sciences, Chonbuk National University) ;
  • Heo, Jun (Department of Biological Sciences, Chonbuk National University) ;
  • Jeon, SaeBom (Department of Biological Sciences, Chonbuk National University) ;
  • Kim, Young Sang (Ministry of Food and Drug Safety (MFDS)) ;
  • Jeong, DoYoun (Microbial Institute for Fermentation Industry (MIFI)) ;
  • Uhm, Tai-Boong (Department of Biological Sciences, Chonbuk National University)
  • 오현화 (전북대학교 자연과학대학 생물학과) ;
  • 류명선 (전북대학교 자연과학대학 생물학과) ;
  • 허준 (전북대학교 자연과학대학 생물학과) ;
  • 전새봄 (전북대학교 자연과학대학 생물학과) ;
  • 김용상 (식품의약품안전처) ;
  • 정도연 (발효미생물산업진흥원) ;
  • 엄태붕 (전북대학교 자연과학대학 생물학과)
  • Received : 2014.08.29
  • Accepted : 2014.09.24
  • Published : 2014.12.31
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Abstract

Two bacterial strains, named as LE17 and LE22, were isolated from traditionally fermented soybean products in order to select lactic acid bacteria for the reduction of biogenic amines and harmful bacteria. Both strains were identified as Pediococcus pentosaceus by 16S rRNA sequence analysis and additional biochemical tests. The strain LE17 reduced the amines by 13.7% for histamine and by 25.9% for tyramine, when it grew in minimal synthetic media containing 0.1% (w/v) histamine and 0.1% tyramine at $30^{\circ}C$ for 48 h, while the strain LE22 reduced the amines by 23.7% for histamine and by 15.7% for tyramine. Both strains also had broad inhibition spectra against pathogens. Considering their properties, they could be used as starters for industrial soybean fermentation.

장류 식품에서 biogenic amines 저감 기능과 유해균 저해 능력을 동시에 지닌 유산균을 선발하기 위해, 전통방식으로 제조한 장류 시료로부터 2종의 균주를 분리하였다. 생화학적 동정 및 16S rRNA 유전자 염기 서열을 분석 결과 이들 균은 유산균인 Pediococcus pentosaceus로 동정되었다. 질소원으로 0.1% (w/v) histamine과 0.1% tyramine이 첨가된 최소 합성 배지에서 $30^{\circ}C$, 48시간 배양 후 잔류 amine을 분석한 결과, LE22 균주의 경우 histamine은 23.7%, tyramine은 15.7%가 감소한 반면, LE17의 경우 histamine은 13.7%, tyramine은 25.9%가 감소하였다. 장류에서 발견되는 주요 유해균에 대한 항균 효과를 조사한 결과, 두 균주 모두 유해균들에 대해 항균 작용을 보였다. 두 균주의 발효 특성을 고려했을 때 이들은 유산균 종균으로써 산업적 장류 생산에 적용할 수 있을 것으로 보인다.

Keywords

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