Journal of the Korean Society of International Agriculture (한국국제농업개발학회지)

The Korean Society of International Agriculture (한국국제농업개발학회)
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Changes in Antioxidant and Antimicrobial Activities of Schizandra chinensis Baillon under Different Solvent Extraction

추출용매별 오미자의 항산화 및 항균 활성 변화

  • Nam, Song Yee (Functional Food & Nutrition Division, Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Jin Young (Functional Food & Nutrition Division, Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Ko, Jeong Sook (Functional Food & Nutrition Division, Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jung Bong (Functional Food & Nutrition Division, Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Jang, Hwan Hee (Functional Food & Nutrition Division, Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Haeng Ran (Functional Food & Nutrition Division, Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Young Min (Functional Food & Nutrition Division, Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration)
  • 남송이 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 이진영 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 고정숙 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 김정봉 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 장환희 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 김행란 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 이영민 (농촌진흥청 국립농업과학원 기능성식품과)
  • Received : 2013.11.11
  • Accepted : 2014.10.24
  • Published : 2014.12.30
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Abstract

The present study was performed to investigate antioxidant and antimicrobial activities of Schizandra chinensis Baillon under different extraction conditions. Extraction yield of water extract, 70% ethanol extract, and methanol extract from S. chinensis was 17.7, 32.9, and 36.6% respectively. Total polyphenol content was 9.2-16.3 mg/g in S. chinensis extracts and was increased significantly by ethanol and methanol extraction (p < 0.05). Radical scavenging activity against DPPH and $ABTS^{{\cdot}+}$ was significantly higher in ethanol and methanol extracts than in water extract (p < 0.05). Ethanol and methanol extracts from S. chinensis also showed effective superoxide dismutase-like activity compared with water extract (p < 0.05). S. chinensis water extract exhibited strong antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium, which was increased significantly by ethanol and methanol extraction (p < 0.05). On the other hand, inhibitory effect of S. chinensis water extract on nitric oxide production was more effective than ethanol and methanol extract.

본 연구에서는 오미자 추출물이 보유하고 있는 것으로 알려진 항산화, 항균, 항염증 활성의 용매별 추출에 따른 변화를 구명하고 관련 기초자료를 제공하고자 하였다. 오미자를 물과 70% 에탄올, 99.9% 메탄올로 추출한 후, 폴리페놀 함량, 라디칼 소거능, SOD 유사활성, 항균활성, NO 생성 저해능을 평가하였다. 그 결과, 오미자 물 추출물의 폴리페놀 함량은 9.2 mg/g이었고 에탄올과 메탄올 추출에 의해서 각각 70.7%, 77.2% 증가하였다. DPPH 라디칼 소거능은 에탄올 추출물에서 $IC_{50}$이 $399.7{\mu}g/mL$으로 항산화능이 가장 높았고, 메탄올 추출물($400.8{\mu}g/mL$), 물 추출물($992.4{\mu}g/mL$)의 순으로 나타났다. 이를 통해, 물 추출물보다 유기용매 추출물의 폴리페놀함량이 높음에 따라 소거활성이 증가하여, 폴리페놀 함량과 라디칼 소거활성은 연관성이 있음을 확인하였다. 병원균 3종 E. coli, S. typhimurium, St. aureus에 대한 항균 활성은 물 추출물의 경우 38.9~64.5%인 반면 유기용매 추출물에서는 70.0 ~ 85.6%로 증가하였다. 반면에 NO 생성에 대해서는 물 추출물이 대조군에 비해 35.7% 저해시켰으나 에탄올과 메탄올 추출에 의해서 각각 22.1%, 25.7% 감소하였다. 결론적으로 오미자의 유기용매(에탄올, 메탄올) 추출물은 물 추출물에 비하여 라디칼 소거능과 SOD 유사 활성, 항균 활성이 우수하였다. 반면에 항염증능은 유기용매 추출물에 비해 물 추출물이 우수한 활성을 보였다. 본 실험결과는 오미자 추출물을 건강기능식품 개발 소재나 가공식품으로 활용할 때 주요 대상으로 하는 건강기능성을 증진시키는데 기초자료로 활용할 수 있을 것으로 생각된다.

Keywords

Acknowledgement

Supported by : 농촌진흥청 국립농업과학원

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