Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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Effects of Nelumbo nucifera Root Extract on Proliferation and Apoptosis in HT-29 Human Colon Cancer Cells

연근(Nelumbo nucifera Root) 추출물의 HT-29 인체 대장암세포 증식 억제 및 사멸 효과

  • Guon, Tae-Eun (Dept. of Food and Nutrition, Duksung Women's University) ;
  • Chung, Ha Sook (Dept. of Food and Nutrition, Duksung Women's University)
  • 권태은 (덕성여자대학교 식품영양학과) ;
  • 정하숙 (덕성여자대학교 식품영양학과)
  • Received : 2014.01.16
  • Accepted : 2014.02.15
  • Published : 2014.02.28
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Abstract

Our study is investigated the effects of Nelumbo nucifera root extract on HT-29 colon cancer cells. The anti-proliferative effect of 70% ethanol extract from Nelumbo nucifera root on HT-29 colon cancer cells was identified based on cell viability, Hoechst 33342 nuclear staining, apoptosis analysis, Western blotting and RT-PCR analyses. In our study, Nelumbo nucifera root extract inhibited the growth of HT-29 colon cancer cells in a dose-dependent manner. Concomitant activation of the mitochondria-dependent apoptotic pathway of HT-29 colon cancer cells by Nelumbo nucifera root extract occurred via modulation of Bax and Bcl-2 expressions, which activated cleavage of caspases-3 and -9. The findings of this study indicate that Nelumbo nucifera root extract induces apoptosis in HT-29 colon cancer cells, and this phenomenon is occurs via the death receptor-mediated and mitochondria-mediated apoptotic pathways.

Keywords

References

  1. Adams JM, Cory S (1998) The Bcl-2 protein family: arbiters of cell survival. Sci 281: 1322-1326. https://doi.org/10.1126/science.281.5381.1322
  2. Chae YH, Shin DY, Park C, Lee YT, Moon SG, Choi YH (2011) Induction of apoptosis in human colon carcinoma HCT116 cells using a water extract of Lepidium virginicum L. J Korean Soc Food Sci Nutr 40: 649-659. https://doi.org/10.3746/jkfn.2011.40.5.649
  3. Cho SI, Kim HW (2003) Beneficial effect of Nodus neoumbinis rhizomatis extracts on cisplatin-induced kidney toxicity in rats. J Korean Herbology 18: 127-134.
  4. Chung HS (2013) Identification of functionalities of HANSIK with cytoprotective activities on human colon cancer cell and standardization of recipies. Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. pp 28-34.
  5. Cory S, Huang DC, Adams JM (2003) The Bcl-2 family: Roles in cell survival and oncogenesis. Oncogene 22: 8590-8607. https://doi.org/10.1038/sj.onc.1207102
  6. Debatin KM (2004) Apoptosis pathways in cancer and cancer therapy. Cancer Immunol Immunother 53: 153-159. https://doi.org/10.1007/s00262-003-0474-8
  7. Han W, Hu WC, Lee YM (2011) Anticancer activity of human colon cancer(HT-29) cell line from different fraction of Zanthoxylum schnifolium fruits. Korean J Pharmacog 42: 282-287.
  8. Hwang YJ, Nam HK, Chang MJ, Noh GW, Kim SH (2003) Effect of Lentinus edodes and Pleurotus eryngii extracts on proliferation and apoptosis in human colon cancer cell lines. J Korean Soc Food Sci Nutr 32: 217-222. https://doi.org/10.3746/jkfn.2003.32.2.217
  9. Jung HA, Kim JE, Chung HY, Choi JS (2003) Antioxidant principles of Nelumbo nucifera stamens. Arch Pharm Res 26: 279-285. https://doi.org/10.1007/BF02976956
  10. Kasumi M, Sakuma F (1998) Flowering, pollination, fertilization, and seed formation in lotus rhizome plant. Japan J Soc Horticul Sci 67: 595-599. https://doi.org/10.2503/jjshs.67.595
  11. Kim EJ, Park HS, Shin MJ, Shin HK, Yoon Park-JH (2009a) Induction of apoptosis in HT-29 human colon cancer cells by the pepper component piperine. J Korean Soc Food Sci Nutr 38: 442-450. https://doi.org/10.3746/jkfn.2009.38.4.442
  12. Kim EJ, Park SY, Hong JU, Shin MJ, Lim SS, Shin HK, Park HY (2007) Inhibitory effect of the methanol extract of Symphyocladia latiuscula on the growth of HT-29 human colon cancer cells. J Korean Soc Food Sci Nutr 36: 431-438. https://doi.org/10.3746/jkfn.2007.36.4.431
  13. Kim MS, Kim IA, Ko YJ, Jeong JA, Kim JE, Song BJ, Won WB, Lim HS, Park JI, Kim SY, Choi HR, Kim OJ (2009b) Methanol extract of leaves from Cudrania tricuspidata effects in HT-29 colorectal adenocarcinoma. Korean J Oral Maxillofacial Pathology 3: 9-26.
  14. Kim YS, Jeon SS, Jung ST (2002) Effect of lotus root powder on the baking quality of white bread. J Korean Soc Food Cookery Sci 18: 413-425.
  15. Ko, BS, Jun DW, Jang JS, Kim JH, Park S (2006) Effect of Sasa borealis and white lotus roots and leaves on insulin action and secretion in vivo. J Korean Food Sci Technol 38: 114-120.
  16. Koesmeyer SJ, Shutter JR, Veis DJ, Merry DE, Oltvai ZN (1993) Bcl-2/Bax: a rheostat that regulates an anti-oxidant patyway and cell death. Semin Cancer Biol 4: 327-332.
  17. Korsmeyer SJ (1995). Regulators of cell death. Trends in Genetics 11: 101-105. https://doi.org/10.1016/S0168-9525(00)89010-1
  18. Krishnaswamy K, Raghuramulu N (1998) Bioactive phytochemicals with emphasis on dietary practices. Indian J Med Res 108: 167-181.
  19. Lee, JJ, Park SE, Lee MY (2006) Effects of lotus root(Nelumbo nucifera G.) on lipid metabolism in rats with diet-induced hypercholesterolemia. J Korean Food Preservation 13: 634-642.
  20. Lee YJ, Hwang IG, Joung EM, Kim-HY, Park ES, Woo KS, Jeong HS (2009) Physiological activity and antiproliferation effects of citron seed extracts on cancer cells. J Korean Soc Food Sci Nutr 38: 1672-1678. https://doi.org/10.3746/jkfn.2009.38.12.1672
  21. Park, SH, Hahm TS, Han JH (2005) Effects of ethanol-extract of lotus root on the renal function and blood pressure of fructose-induced hypertensive rats. J East Asian Soc Dietary Life 15: 165-170.
  22. Rajamanickam S, Agarwal R (2008) Natural products and colon cancer: Current status and future prospects. Drug Dev Res. 69: 460-471. https://doi.org/10.1002/ddr.20276
  23. Rajendra K, Rhyu DY, Kim DW (2008) Effect of Nelumbo nucifera on proliferation, migration and expression of MMP- 2 and MMP-9 of rSMC, A431 and MDA-MB-231. J Korean Plant Res 21: 96-102.
  24. Reed JC (1998a) Dysregulation of apoptosis in cancer. Cancer J Sci Am Suppl 1: S8-14.
  25. Reed JC (1998b) Bcl-2 family proteins. Oncogene 17: 3225-3236.
  26. Ryu MJ, Chung HS (2011) Effects on hot water extract of Schizandra chinensis on colon cancer cell line. Food Eng Prog 15: 64-69.
  27. Ryu MJ, Kim AD, Kang KA, Chung HS, Suh IS, Chang WY, Hyun JW (2013) The green algae Ulva fasciata Delile extract induces apoptotic cell death in human colon cancer cells. In Vitro Cell Dev Biol Anim 49: 74-81. https://doi.org/10.1007/s11626-012-9547-3
  28. Ryu MJ, Kang KA, Piao MJ, Kim KC, Zheng J, Yao CW, Cha JW, Chung HS, Kim SC, Jung ES, Park DH, Chae SW, Hyun JW (2014) 7,8-Dihydroxyflavone protects human keratinocytes against oxidative stress-induced cell damage via the ERK and PI3K/Akt-mediated Nrf2/HO-1 signaling pathways. Inter J Mol Med 33: 964-970.
  29. Sandler RS, Drossman DA (1987) Bowel habits in young adults not seeking health care. Dig Dis Sci 32: 841-845. https://doi.org/10.1007/BF01296706
  30. Shin HR, Jung KW, Won YJ, Park JG (2004) 2002 Annual report of the Korea Central Cancer Registry: Based on registered data from 139 hospitals. Cancer Res Treatment 36: 103-114. https://doi.org/10.4143/crt.2004.36.2.103
  31. Shin JH, Jung KM, Lee SJ, Yang SM, Rue GH, Sung NJ (2009) Biological activities of dried garlic, red ginseng and their mixture. J Korean Soc Food Sci Nutr 38: 1633-1639. https://doi.org/10.3746/jkfn.2009.38.12.1633
  32. Villa P, Kaufmann SH, Earnshaw WC (1997) Caspases and caspase inhibitors. Trends in Biochem Sci 22: 388-393. https://doi.org/10.1016/S0968-0004(97)01107-9