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Antioxidant Contents and Antioxidant Activities of Hot-Water Extracts of Aronia (Aronia melancocarpa) with Different Drying Methods

건조방법에 따른 아로니아(Aronia melancocarpa) 열수 추출물의 항산화 성분 함량 및 항산화 활성

  • Hwang, Eun-Sun (Department of Nutrition and Culinary Science, Hankyong National University) ;
  • Thi, Nhuan Do (Department of Nutrition and Culinary Science, Hankyong National University)
  • Received : 2014.01.08
  • Accepted : 2014.04.04
  • Published : 2014.06.30

Abstract

This study determined the antioxidant levels and activities of hot water aronia extracts by different drying methods such as sun drying, sun drying after steam treatment, freeze-drying, and oven drying. The total polyphenol content, calculated as gallic acid equivalent, was the highest in the freeze-dried sample (910 mg), followed by sun-dried after steam treatment (779 mg), sun-dried (769 mg), and oven-dried (757 mg) samples. Similar patterns were observed for the total flavonoid and anthocyanin contents. Freeze-dried aronia samples contained the highest polyphenol, flavonoid, and anthocyanin contents as compared to the samples dried by other methods. All antioxidant activities were found to increase in a dose-dependent manner. For the hot water-extracted freeze-dried aronia powder (200 mg/mL), the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radicalscavenging activities were 65.5% and 61.7% and the hydroxyl and superoxide anion radical-scavenging activities were 50.5% and 52.1%, respectively. These results suggest that comparatively, freeze-drying is a better method for preserving the bioactive components and the antioxidant activities of aronia.

아로니아 열수추출물 분말을 제조하여 가공식품에 적용하기 위한 방법을 모색하기 위하여 다양한 건조방법이 아로니아의 항산화 성분 함량 및 항산화 활성에 미치는 영향을 알아보았다. 신선한 아로니아를 일광건조, 스팀 후 일광건조, 동결건조 및 오븐건조의 방법으로 완전히 건조시킨 후 분말로 제조하여 $100^{\circ}C$에서 6시간 동안 3회 반복 열수 추출하여 총 폴리페놀, 플라보노이드 및 안토시아닌 함량을 분석하였다. 아로니아의 총 폴리페놀 함량(mg gallic acid/g 기준)은 동결건조한 시료에서 가장 높았으며, 스팀 후 일광건조 > 일광건조 > 및 오븐건조의 순으로 높게 나타났다. 아로니아의 총 플라보노이드 함량은 quercetin을 기준으로 동결건조한 시료에서 가장 높았으며, 스팀 후 일광건조 > 일광건조 > 오븐건조의 순으로 나타났다. 아로니아에 함유된 총 안토시아닌 함량은 cyanidin-3-glucoside를 기준으로 동결건조 > 일광건조 > 스팀 후 일광건조 > 오븐건조의 순으로 나타났다. 아로니아 열수추출물의 항산화 활성은 DPPH 라디칼 소거능, ABTS 라디칼 소거능, hydroxyl 라디칼 소거능, superoxide anion 라디칼 소거능 및 환원력의 5가지 방법으로 측정하였다. 4가지 건조방법 중에서 동결건조 시료에서 항산화 활성이 유의적으로 높게 나타났으며, 오븐건조한 시료에서 가장 낮게 나타났다. 이상의 결과를 통하여 건조방법이 아로니아의 항산화 성분 함량과 항산화 활성에 영향을 주며 고온의 건조방법보다는 저온의 동결건조 방법이 아로니아의 유효성분 함량과 항산화 활성을 증대시키기 위한 최적의 방법으로 사료된다.

Keywords

References

  1. Bohr VA. Repair of oxidatve DNA damage in nuclear and mitochondrial DNA, and some changes with aging in mammalian cells. Free Radic. Biol. Med. 32: 804-812 (2002) https://doi.org/10.1016/S0891-5849(02)00787-6
  2. Seitz HK, Stickel F. Risk factors and mechanisms of hepatocarcinogenesis with special emphasis on alcohol and oxidative stress. Biol. Chem. 387: 349-360 (2006)
  3. Go VL, Wong DA, Wang Y, Butrum RR, Norman HA, Wilkerson L. Diet and cancer prevention: evidence-based medicine to genomic medicine. J. Nutr. 134: 3513S-3516S (2004)
  4. Hung HC, Joshipura KJ, Jiang R, Hu FB, Hunter D, Smith-Warner SA, Colditz GA, Rosner B, Spiegelman D, Willett WC. Fruit and vegetable intake and risk of major chronic disease. J. Natl. Cancer Inst. 96: 1577-1584 (2004) https://doi.org/10.1093/jnci/djh296
  5. Das DK, Sato M, Ray PS, Maulik G, Engleman RM, Bertelli AAE, Bertelli A. Cardioprotection of red wine: Role of polyphenolic antioxidants. Drug Exp. Clin. Res. 25: 115-120 (1999)
  6. Ahn J, Gammon MD, Santella RM, Gaudet MM, Britton JA, Teitelbaum SL, Terry MB, Nowell S, Davis W, Garza C, Neugut AI, Ambrosone CB. Associations between breast cancer risk and the catalase genotype, fruit and vegetable consumption, and supplement use. Am. J. Epidemiol. 162: 943-952 (2005) https://doi.org/10.1093/aje/kwi306
  7. Wu X, Gu L, Prior RL, McKay S. Characterization of anthocyanins and proanthocyanidins some cultivars of Ribes, Aronia, and Sambucus and their antioxidant capacity. J. Agr. Food Chem. 52: 7846-7856 (2004) https://doi.org/10.1021/jf0486850
  8. Sueiro L, Yousef GG, Seigler D, De Mejia EG, Grace MH, Lila MA. Chemopreventive potential of flavonoid extracts from plantation-bred and wild Aronia melancocarpa (black chokeberry) fruit. J. Food Sci. 71: C480-C488 (2006) https://doi.org/10.1111/j.1750-3841.2006.00152.x
  9. Oszmianski J, Wojdylo A. Aronia melancocarpa phenolics and their antioxidant activity. Eur. Food Res. Technol. 221: 809-813 (2005) https://doi.org/10.1007/s00217-005-0002-5
  10. Kim B, Ku CS, Pham TX, Park Y, Martin DA, Xie L, Taheri R, Lee J, Bolling BW, Aronia melancocarpa (chokeberry) polyphenol-rich extract improves antioxidant function and reduces total plasma cholesterol in apolipoprotein E knockout mice. Nutr. Res. 33: 406-413 (2013) https://doi.org/10.1016/j.nutres.2013.03.001
  11. Sharif T, Stambouli M, Burrus B, Emhemmed F, Dandache I, Auger C, Etienne-Selloum N, Schini-Kerth VB, Fuhrmann G. The polyphenolic-rich Aronia melancocarpa juice kills teratocarcinomal cancer stem-like cells, but not their differentiated counterparts. J. Funct. Food 5: 1244-1252 (2013) https://doi.org/10.1016/j.jff.2013.04.007
  12. Bridle P, Timberlake CF. Anthocyanins as natural food colorsselected aspects. Food Chem. 58: 103-109 (1997) https://doi.org/10.1016/S0308-8146(96)00222-1
  13. Kurozawa LE, Terng I, Hubinger MD, Park KJ. Ascorbic acid degradation of papaya during drying: Effect of process conditions and glass transition phenomenon. J. Food Eng. 123: 157-164 (2014) https://doi.org/10.1016/j.jfoodeng.2013.08.039
  14. Ezhilarasi PN, Indrani D, Jena BS, Anandharamakrishnan C. Freeze drying technique for microencapsulation of Garcinia fruit extract and its effect on bread quality. J. Food Eng. 117: 513-520 (2013) https://doi.org/10.1016/j.jfoodeng.2013.01.009
  15. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Method Enzymol. 299: 152-178 (1999) https://doi.org/10.1016/S0076-6879(99)99017-1
  16. Giusti MM, Wrolstad RE. Characterization and measurement of anthocyanins by UV-visible spectroscopy. pp. 19-31. In: Handbook of Food Analytical Chemistry. John Wiley & Sons, Hoboken, NJ, USA (2001)
  17. Cheung LM, Cheung Peter CK, Ooi Vincent EC. Antioxidant activity and total pehnolics of edible mushroom extracts. Food Chem. 81: 249-255 (2003) https://doi.org/10.1016/S0308-8146(02)00419-3
  18. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237 (1999) https://doi.org/10.1016/S0891-5849(98)00315-3
  19. Chung SK, Osawa T, Kawakishi S. Hydroxyl radical-scavenging effects of spices and scavengers from brown mustard (Brassica nigra). Biosci. Biotech. Biochem. 61: 118-123 (1997) https://doi.org/10.1271/bbb.61.118
  20. Wang J, Yuan X, Jin Z, Tian Y, Song H. Free radical and reactive oxygen species scavenigng activities of peanut skins extract. Food Chem. 104: 242-250 (2007) https://doi.org/10.1016/j.foodchem.2006.11.035
  21. Oyaizu M. Studies on product of browning reaction. Antioxidative activities of products of browning reaction prepared from glucoseamine. Japanese. J. Nutr. Diet. 44: 307-315 (1986) https://doi.org/10.5264/eiyogakuzashi.44.307
  22. Jeong JM. Antioxidative and antiallergic effects of aronia (Aronia melancocarpa) extract. J. Korean Soc. Food Sci. Nutr. 37: 1109-1113 (2008) https://doi.org/10.3746/jkfn.2008.37.9.1109
  23. Park YK, Cho SH, Kim SH, Jang YS, Han JY, Chun HG. Functional composition and antioxidant activity from the fruits Rubus coreanus according to cultivars. Mokchae Konghak 36: 102-109 (2008) https://doi.org/10.5658/WOOD.2008.36.1.102
  24. Kim JM, Shin M. Characteristics of Rubus coreanus Miq. fruits at different ripening stages. Korean J. Food Sci. Technol. 43: 341-347 (2011) https://doi.org/10.9721/KJFST.2011.43.3.341
  25. Hakkinen S, Heinonen M, Karenlampi S, Mykkanen H, Ruuskanen J, Torronen R. Screening of selected flavonoids and phenolic acids in 19 berries. Food Res. Int. 32: 345-353 (1999) https://doi.org/10.1016/S0963-9969(99)00095-2
  26. Kim MJ, Kim IJ, Nam SY, Lee CH, Un T, Song BH. Effects of drying methods on content of active components, antioxidant activity, and color values of Saururus chinensis Bail. Korean J. Med. Crop Sci. 14: 8-13 (2006)
  27. Kim MJ, Chu WM, Park EJ. Antioxidant and antigenotoxic effects of shiitake mushrooms affected by different drying methods. J. Korean Soc. Food Sci. Nutr. 41: 1041-1048 (2012) https://doi.org/10.3746/jkfn.2012.41.8.1041
  28. Peleg H, Naim M, Rouseff RL, Zehavi U. Distribution of bound and free phenolic acids in oranges (Citrus sinensis) and grapefruit (Citrus paradisi). J. Sci. Food Agr. 57: 417-426 (1991) https://doi.org/10.1002/jsfa.2740570312
  29. Yook HS, Kim KH, Jang SA. Quality characteristics of grape pomace with different drying methods. J. Korean Soc. Food Sci. Nutr. 39: 1353-1358 (2010) https://doi.org/10.3746/jkfn.2010.39.9.1353
  30. Thongchai W, Liawruangrath B, Liawruangrath S. Flow injection analysis of total curcuminoids in turmeric and antioxidant capacity using 2,2'-diphenyl-1-picrylhydrazyl assay. Food Chem. 112: 494-499 (2009) https://doi.org/10.1016/j.foodchem.2008.05.083
  31. Hochstein P, Atallah AS. The nature of oxidants and antioxidant systems in the inhibition of mutation and cancer. Mutat. Res. 202: 363-375 (1988) https://doi.org/10.1016/0027-5107(88)90198-4
  32. Klompong V, Benjakul S, Kantachote D, Shahidi F. Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type. Food Chem. 102: 1317-1327 (2007) https://doi.org/10.1016/j.foodchem.2006.07.016
  33. Jeong CH, Nam EK, Shim KH. Antioxidative activities and nitrate scavenging activity in different parts of Erigeron annuus. J. Agric. Life Sci. 40: 13-29 (2006)
  34. Oh HK. Nutritional composition and antioxidative activity of different parts of Taraxacum coreanum according to drying methods. J. Korean Diet. Assoc. 19: 389-399 (2013) https://doi.org/10.14373/JKDA.2013.19.4.389
  35. Choi YM, Yu KW, Han NS, Koh JH, Lee JS. Antioxidant activities and antioxidant compounds of commercial red wines. J. Korean Soc. Food Sci. Nutr. 35: 1286-1290 (2006) https://doi.org/10.3746/jkfn.2006.35.9.1286
  36. Park YK, Choi SH, Kim SH, Han JG, Chung HG. Changes in antioxidant activity, total phenolics and vitamin C content during fruit ripening in Rubus occidentalis. Korean J. Plant Res. 20: 461-465 (2007)
  37. Jeong CH, Choi SG, Heo HJ. Analysis of nutritional compositions and antioxidative activities of Korean commercial blueberry and raspberry. J. Korean Soc. Food Sci. Nutr. 37: 1375-1381 (2008) https://doi.org/10.3746/jkfn.2008.37.11.1375
  38. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. Screening of the antioxidant activity of some medicinal plants. Korean J. Food Sci. Technol. 36: 333-338 (2004)

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