Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Saccharina japonica Extract Protects against Carbon Tetrachloride-induced Hepatotoxicity in Rats

사염화탄소로 유도된 흰쥐의 간손상으로부터 다시마(Saccharina japonica) 추출물의 보호효과

  • Kim, Mi-Kyung (Department of Food Science and Nutrition, Pukyong National University) ;
  • Kim, In-Hye (Department of Food Science and Nutrition, Pukyong National University) ;
  • Nam, Taek-Jeong (Department of Food Science and Nutrition, Pukyong National University)
  • 김미경 (부경대학교 식품영양학과) ;
  • 김인혜 (부경대학교 식품영양학과) ;
  • 남택정 (부경대학교 식품영양학과)
  • Received : 2014.04.16
  • Accepted : 2014.04.30
  • Published : 2014.06.30
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Abstract

The brown alga Saccharina japonica is consumed as a foodstuff in many countries. Carbon tetrachloride ($CCl_4$) is a potent hepatotoxin that is used to assess hepatotoxicity in animal models. This study assessed the protective effect of S. japonica extract (SJE) on $CCl_4$-induced acute liver injury in rats. Experimental rats were divided into the following three groups: control, $CCl_4$, and $CCl_4$+SJE; the latter two groups were given 150 or 300 mg SJE/kg orally for 10 days. Three hours after the final treatment, all rats-except for those in the control group-were administered intraperitoneal injections of $CCl_4$. One day later, blood and liver samples were collected for evaluation biomarker of levels. Aspartate transaminase (APT; GPT) and alanine aminotransferase (ALT; GOT) levels were markedly lower in the $CCl_4$+SJE group than the $CCl_4$ group. The hepatic superoxide dismutase (SOD) activity of the $CCl_4$+SJE group was significantly lower than that of the $CCl_4$ group. In comparison, glutathione S-transferase (GSH) and catalase (CAT) levels were significantly higher in the $CCl_4$+SJE group. Western blotting revealed that SJE attenuated the $CCl_4$-induced EGFR and MAPK activity in the liver. Thus, we conclude that SJE protects against $CCl_4$-induced hepatotoxicity.

Keywords

References

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