Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Identification of Endophytic Bacteria in Panax ginseng Seeds and Their Potential for Plant Growth Promotion

인삼종자로부터 분리된 내생균의 동정과 식물생장 촉진 관련 활성의 평가

  • Um, Yurry (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Bo Ra (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Jeong, Jin Ju (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Chung, Chan Moon (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Lee, Yi (Department of Industrial Plant Science and Technology, Chungbuk National University)
  • 엄유리 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김보라 (충북대학교 특용식물학과) ;
  • 정진주 (충북대학교 특용식물학과) ;
  • 정찬문 (충북대학교 특용식물학과) ;
  • 이이 (충북대학교 특용식물학과)
  • Received : 2014.07.01
  • Accepted : 2014.08.06
  • Published : 2014.08.30
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Abstract

Endophytes are microorganisms that live in the internal tissues of plants without harming the host plants. In this symbiotic relationship, the host plants provide nutrients and shelter to the endophytes, in turn, endophytes can promote the growth of host plants and act as a biological control agents against plant pathogens. Plant-microbe interactions like this are noted for natural methods for sustainable agriculture and environmental conservation. However, in spite of the infinite potential, there are only a few reports on the endophytes present in ginseng. In this study, we isolated and identified the endophytes from Panax ginseng seeds and evaluated the biological activities (IAA production ability, nitrogen fixation ability, phosphate solubilization capacity, siderophore production ability, and antifungal activities) of the endophyte isolates. Eight different endophytes were identified by 16S rRNA sequencing. Most of the endophytes have antibiotic and plant growth promoting (PGP) activities. Particularly, PgSEB5-37E have the highest antibiotic activity, both PgSEB5-37B and PgSEB5-37H have high PGP traits such as an abilities to produce IAA, solubilize phosphate and fix nitrogen. These results indicated that the endophytes from P. ginseng seeds may have applicable value to many industries. In order to use the isolated endophytes, quantitative analysis and field tests are needed to be performed.

Keywords

References

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