Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Screening of Antifungal Bacillus spp. against Alternaria Blight Pathogen (Alternaria panax) and Anthracnose Pathogen (Colletotrichum gloeosporioides) of Ginseng

인삼 점무늬병균(Alternaria panax)과 탄저병균(Colletotrichum gloeosporioides)에 대한 길항미생물 Bacillus spp. 선발

  • 이혜진 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박기춘 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이승호 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 방경환 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박홍우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 현동윤 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 강승원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 정일민 (건국대학교 생명환경과학대학 응용생물과학과)
  • Received : 2012.08.09
  • Accepted : 2012.09.27
  • Published : 2012.10.30
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Abstract

This study was carried out to research microorganisms having the antifungal activity against ginseng Alternaria blight pathogen Alternaria panax and ginseng anthracnose pathogen Colletotrichum gloeosporioides. Eleven Bacillus strains. were isolated from Korean traditional soybean paste and Kimchi. Among the 11 isolates, DJ5, KC1, KC2 and KC4 showing antagonistic activity on the mycelial growth of A. panax and C. gloeosporioides in pairing culture were finally selected as the antagonistic microorganisms. Based on 16s rRNA sequence and phylogenetic tree analysis, they were identified as Bacillus spp.. The selected microorganisms were investigated antagonistic activity by measured leaf-segment colonization in pot test. When Bacillus sp. were injected after A. panax treatment, KC1, KC2 and KC4 showed similar effect to chemical pesticides treated control. To measure preventive effect of Bacillus sp, antagonistic microorganisms were injected and C. gloeosporioides was treated in pot. When measuring the effectiveness for the prevention of Anthracnose, All Bacillus spp. showed approximately 83~90 % degree of superior preventive effect. In general, The four Bacillus spp. isolated from Korean traditional fermented foods showed therapeutic effect of Alternaria blight and preventive effect of Anthracnose.

Keywords

References

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