Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
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Effects of Herbal Medicine By-products on Rumen Fermentation Characteristics In vitro

한약추출 부산물이 in vitro 반추위 발효특성에 미치는 영향

  • Ahn, Seung Kyu (Sancheong oriental medicinal herb institute) ;
  • Goo, Young-Min (Sancheong oriental medicinal herb institute) ;
  • Ko, Keon Hee (Sancheong oriental medicinal herb institute) ;
  • Lee, Shin Ja (Division of Applied Life Science (BK21 program) & Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Moon, Yea Hwang (Department of animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Lee, Sang Suk (Department of animal Science & Technology, Sunchon National University) ;
  • Kim, Jin Wook (Division of Applied Life Science (BK21 program) & Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Lee, Sung Sill (Division of Applied Life Science (BK21 program) & Institute of Agriculture and Life Science (IALS), Gyeongsang National University)
  • 안승규 ((재)산청한방약초연구소) ;
  • 구영민 ((재)산청한방약초연구소) ;
  • 고건희 ((재)산청한방약초연구소) ;
  • 이신자 (경상대학교 응용생명과학부(BK21 program) 농업생명과학연구원) ;
  • 문여황 (경남과학기술대학교 동물생명과학과) ;
  • 이상석 (순천대학교 동물자원과학과) ;
  • 김진욱 (경상대학교 응용생명과학부(BK21 program) 농업생명과학연구원) ;
  • 이성실 (경상대학교 응용생명과학부(BK21 program) 농업생명과학연구원)
  • Received : 2014.01.10
  • Accepted : 2014.03.07
  • Published : 2014.04.30
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Abstract

An in vitro ruminal degradation trial was conducted to evaluate the nutritive values of herbal medicine by-products (HMB). Major ten materials for preparing oriental herbal medicine, Ginseng radix alba (Panax ginseng C. A. Meyer.), Hoelen (Poria cocos Wolf), Glycyrrhizae radix (Glycyrrhiza uralensis Fisch.), Angelicae gigantis radix (Angrlica gigas Nakai), Cnidii rhizoma (Cnidium officinale Makino), Paeoniae radix (Paeonia lactiflora Pall.), Astragali radix (Astragalus membranaceus Bunge), Zingiberis rhizoma crudus (Zingiberis officinale Roscoe), Ziziphi fructus (Ziziphus jujuba var. inermis Mill.), and Aurantii nobilis pericarpium (Citrus unshiu Marcow.) were used in the experiment. The 15 mL of mixture contained McDougall's buffer and rumen fluid in the ratio 2 to 1, was dispensed anaerobically into 50 mL serum bottles. The HMB was added at the level of 3% or 5% against 300 mg of timothy as a substrate (v/w). The serum bottles were incubated at $39^{\circ}C$, 120 rpm for 6, 12, 24, 48 and 72 h with triplicate. Total gas production (55 values) did not differ (P<0.05) between all HMB and control in both two additional levels during whole incubation time, whereas only 4 values including 172.96 (3% Zingiberis rhizoma crudus in 24 h), 232.31 (3% Aurantii nobilis pericarpium in 72 h), 209.87 (5% Aurantii nobilis pericarpium in 48 h) and 188.12 (5% Astragali radix in 72 h) were significantly lower than other 51 values. The microbial growth rate on all incubation except for 48 h incubation tended to be increased by addition of 5% HMB with the statistical differences (P<0.05). The VFA concentration tended to be increased gradually with the increasing incubation times, At the initial stage (6 h) of incubation, total VFA concentration of HMB was increased with the significant differences (P<0.05) except for few HMB treatment (Paeoniae radix, Astragali radix, Aurantii nobilis pericarpium). It is concluded that herbal medicine by-products (HMB) can increase microbial growth rate and improve ruminal fermentation. Therefore, the 5% substitution of HMB for roughage are highly recommended to be used in practice.

한약추출 부산물의 영양가치 및 사료 가치를 평가하기 위해 in vitro 반추위 분해 시험을 하였다. 시험에 사용한 10가지 한약추출 부산물은 인삼(Ginseng radix alba), 백복령(Hoelen), 감초(Glycyrrhizae radix), 당귀(Angelicae gigantis radix), 천궁(Cnidii rhizoma), 작약 (Paeoniae radix), 황기(Astragali radix), 생강(Zingiberis rhizoma crudus), 대추(Ziziphi fructus), 진피(Aurantii nobilis pericarpium)로 한약재 중 빈번하게 사용하는 약재이다. In vitro 시험을 위해 50 mL serum bottle에 혐기상태로 McDougall's buffer와 반추위액을 2:1비율로 15 mL을 넣었다. 한약추출 부산물은 timothy(300 mg)를 기질로 3%, 5% 수준으로 첨가하였다. Serum bottle은 배양 시간대별(6, 12, 24, 48, 및 72 h)로 $39^{\circ}C$에서 120 rpm으로 진탕 배양하였다. 총 가스 발생량은 한약추출 부산물 3% 첨가구에서는 발효 24시간대에서 대조구가 생강(Zingiberis rhizoma crudus)구에 비해 많았으며, 발효 72시간대에서도 대조구가 가장 많았고, 진피(Aurantii nobilis pericarpium)구에서 적었다(P<0.05). 한약추출부산물 5% 첨가구에서는 48시간대 가스 발생량이 진피(Aurantii nobilis pericarpium)구보다 대조구에서 많았으며, 72시간 발효구에서는 황기(Astragali radix)구에서 가장 적었다(P<0.05). 미생물 성장률은 한약추출 부산물 5% 첨가구의 48시간대를 제외한 모든 배양에서 유의적으로 증가하였다(P<0.05). VFA 농도는 배양시간의 따라 증가되는 경향을 보였으며, 배양 초기(6 h)에서 한약추출 부산물의 총 VFA 농도는 일부 한약추출 부산물 처리(작약, 황기, 진피)를 제외하고 유의적으로 증가하였다(P<0.05). 위의 결과로 한약추출 부산물은 미생물 성장률 및 발효성상 개선을 증진시킬 수 있으며, 결론적으로 조사료에 대한 한약추출 부산물 5% 대체가 가능할 것으로 예상된다.

Keywords

Acknowledgement

Supported by : 농촌진흥청

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