Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Isolation and Antioxidant Activity of Methyl Aconitates from Arctic Red Alga Polysiphonia stricta

극지 홍조류 Polysiphonia stricta에서 분리된 methyl trans-aconitate 유도체들과 항산화 활성

  • Lee, Jung Im (Division of Marine Environment & Bioscience, College of Maritime Sciences Korea Maritime and Ocean University) ;
  • Kong, Chang-Suk (Department of Food and Nutrition, College of Medical and Life Sciences Silla University) ;
  • Baek, Seung Oh (Division of Marine Environment & Bioscience, College of Maritime Sciences Korea Maritime and Ocean University) ;
  • Seo, Youngwan (Division of Marine Environment & Bioscience, College of Maritime Sciences Korea Maritime and Ocean University)
  • 이정임 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 공창숙 (신라대학교 의생명과학대학 식품영양학과) ;
  • 백승오 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 서영완 (한국해양대학교 해양과학기술대학 해양환경.생명과학부)
  • Received : 2014.08.27
  • Accepted : 2014.09.17
  • Published : 2014.09.30
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Abstract

In our continuing study on the antioxidant activity of Polysiphonia stricta, its crude extract was fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions according to solvent polarity. The solvent fractions were evaluated for their potential to inhibit lipid peroxidation and reactive oxygen species (ROS) production in HT 1080 cells. The n-BuOH fraction most strongly inhibited both lipid peroxidation and ROS production in HT 1080 cells. The n-BuOH fraction was further separated by repeated silica gel column chromatography and RP-HPLC to give methyl aconitates (2 and 3). The chemical structure of isolated compounds was determinated by NMR spectral analysis.

Keywords

References

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