The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Four Species of Endophytic Fungi Isolated from Leaves of Woody Plants in Mt. Hambaek

강원도 함백산에 서식하는 목본식물의 잎에서 분리한 4종의 내생균

  • Eo, Ju-Kyeong (Department of Biology Education, Korea National University of Education) ;
  • Lee, Bong-Hyung (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 어주경 (한국교원대학교 생물교육과) ;
  • 이봉형 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2014.09.18
  • Accepted : 2014.09.23
  • Published : 2014.09.30
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Abstract

In this study, endophytic fungi were isolated from leaves of four species of woody plants, Acer tagmentosum, Larix kaempferi, Abies holophylla, and Pinus koraiensis, on Mt. Hambaek, Gangwondo, Korea. The endophytic fungi were identified using morphological and sequences analysis of ITS regions. The fungal endophytes were identified as Talaromyces radicus, Myceliophthora verrucosa, Cryptosporiopsis diversispora, and Sphaerulina berberidis, which are the first record in Korea. The morphological and molecular phylogenetic characteristics of each strain were described.

본 연구에서는 강원도 함백산에 서식하는 잣나무, 전나무, 일본잎갈나무, 산겨릅나무 등 4종의 목본식물의 잎에서 내생균을 분리하여 균의 형태적 특징과 ITS 부위의 염기서열을 이용하여 분자계통 분석을 실시하였다, 그 결과, 국내 미기록종인 Talaromyces radicus, Myceliophthora verrucosa, Cryptosporiopsis diversispora, Sphaerulina berberidis로 동정되었으며, 각 균주의 형태학적, 분자계통학적 특징을 기술하였다.

Keywords

References

  1. Carroll G. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9. https://doi.org/10.2307/1943154
  2. Eo JK, Choi MS, Eom AH. Diversity of endophytic fungi isolated from Korean ginseng leaves. Mycobiology 2014;42:147-51. https://doi.org/10.5941/MYCO.2014.42.2.147
  3. Kim CK, Eo JK, Eom AH. Diversity and seasonal variation of endophytic fungi isolated from three conifers in Mt. Taehwa, Korea. Mycobiology 2013;41:82-5. https://doi.org/10.5941/MYCO.2013.41.2.82
  4. Gardes M, Bruns T. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  5. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9. https://doi.org/10.1093/molbev/msr121
  6. Kim CK, Eo JK, Eom AH. Diversity of endophytic fuingi isolated from leaves of coniferous trees in Mt. Minjuji, Korea. Kor J Mycol 2014;42:174-7. https://doi.org/10.4489/KJM.2014.42.2.174
  7. Hoffman MT, Arnold AE. Geographic locality and host identity shape fungal endophyte communities in cupressaceous trees. Mycol Res 2008;112:331-44. https://doi.org/10.1016/j.mycres.2007.10.014
  8. Arnold AE. Understanding the diversity of foliar endophytic fungi: progress, challenges, and frontiers. Fungal Biol Rev 2007;21:51-66. https://doi.org/10.1016/j.fbr.2007.05.003
  9. Ailsa DH, Melanie W, Terence JH. Penicillium radicum sp. nov. from the rhizosphere of Australian wheat. Mycol Res 1998;102:801-6. https://doi.org/10.1017/S0953756297005698
  10. Stephen W. P, Zeljko J. Talaromyces columbinus sp. nov., and genealogical concordance analysis in Talaromyces Clade 2a. PLoS ONE 2013;8(10):e78084. doi:10.1371/journal.pone.0078084

Cited by

  1. Species List of Aspergillus, Penicillium and Talaromyces in Korea, Based on ‘One Fungus One Name’ System vol.44, pp.4, 2016, https://doi.org/10.4489/KJM.2016.44.4.207