The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effect of Glycyrrhizae Radix Water Extract on the depression induced by LPS in Rats

LPS로 유발된 우울증에 대한 감초(甘草)의 효과

  • Ko, Sung-Youl (Department of Formulae Pharmacology, School of Oriental Medicine, Gachon University) ;
  • Kim, Do-Hoon (Department of Oriental Medical Classics & History, Gachon University) ;
  • Lee, Tae-Hee (Department of Formulae Pharmacology, School of Oriental Medicine, Gachon University)
  • 고성렬 (가천대학교 한의과대학 방제학교실) ;
  • 김도훈 (가천대학교 한의과대학 원전의사학교실) ;
  • 이태희 (가천대학교 한의과대학 방제학교실)
  • Received : 2014.05.09
  • Accepted : 2014.09.11
  • Published : 2014.09.30
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Abstract

Objectives : Investigation of the antidepressant effect of Glycyrrhizae Radix (GR) through the anti-inflammatory effect. Methods : Depression in rats was induced by LPS (i,p.3days). The rats were treated with GR100 mg/kg (GR 100) or GR400 mg/kg (GR 400). The depressive immobility was examined with Tail Suspension Test(TST) and Forced Swimming Test(FST). The expression of nuclear factor-${\kappa}B$(NF-${\kappa}B)$, $I{\kappa}B$ was measured with western blotting. The concentration of corticosterone, cytokine in plasma was measured with ELISA. The expression of c-Fos in the paraventricular nucleus(PVN) and tyrosine hydroxylase(TH) in the locus coeluleus(LC) were measured with immunostaining method. Results : In the TST, GR400 group significantly decreased immobility time compared with the LPS group. In the FST, GR100, GR400 group significantly decreased immobility time comparing with the LPS group. c-Fos expression in GR100 and GR400 group was decreased comparing with the lipoplysaccharide(LPS) group. The $I{\kappa}B$ expression of GR100 and GR400 group was increased comparing with the LPS group. The level of corticosterone of GR100 group was decreased comparing with the LPS group. The concentration of cytokine of GR100 and GR400 group was decreased comparing with the LPS group. TH expression in the LC was increased in LPS group, but in GR100 and GR400 group was not shown significant decrease. Conclusion : According to this results obtained, GR has antidepressant effects by the anti inflammatory action through the suppression of HPA axis activity, not through the action against the catecholaminergic system.

Keywords

References

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