Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Proteomics Analysis of Early Salt-Responsive Proteins in Ginseng (Panax ginseng C. A. Meyer) Leaves

초기 염류 스트레스 반응 인삼 잎 단백질체 분석

  • Kim, So Wun (Department of Plant Bioscience, Pusan National University) ;
  • Min, Chul Woo (Department of Plant Bioscience, Pusan National University) ;
  • Gupta, Ravi (Department of Plant Bioscience, Pusan National University) ;
  • Jo, Ick Hyun (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA) ;
  • Bang, Kyong Hwan (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Young-Chang (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Kee-Hong (Ginseng Research Division, Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Sun Tae (Department of Plant Bioscience, Pusan National University)
  • 김소운 (부산대학교 식물생명과학과) ;
  • 민철우 (부산대학교 식물생명과학과) ;
  • ;
  • 조익현 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 방경환 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영창 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김기홍 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김선태 (부산대학교 식물생명과학과)
  • Received : 2014.09.17
  • Accepted : 2014.09.24
  • Published : 2014.10.30
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Abstract

Salt stress is one of the major abiotic stresses affecting the yield of ginseng (Panax ginseng C. A. Meyer). The objective of this study was to identify bio-marker, which is early responsive in salt stress in ginseng, using proteomics approach. Ginseng plants were exposed to 5 ds/m salt concentration and samples were harvested at 0, 6, 12 and 18 hours after exposure. Total proteins were extracted from ginseng leaves treated with salt stress using Mg/NP-40 buffer and were separated on high resolution 2-DE. Approximately $1003{\pm}240$ (0 h), $992{\pm}166$ (6 h), $1051{\pm}51$ (12 h) and $990{\pm}160$ (18 h) spots were detected in colloidal CBB stained 2D maps. Among these, 8 spots were differentially expressed and were identified by using MALDI-TOF/TOF MS or/and LC-MS/MS. Ethylene response sensor-1 (spot GL 1), nucleotide binding protein (spot GL 2), carbonic anhydrase-1 (spot GL 3), thylakoid lumenal 17.9 kDa protein (spot GL 4) and Chlorophyll a/b binding protein (spot GL 5, GL 6) were up-regulated at the 12 and 18 hour, while RuBisCO activase B (spot GL 7) and DNA helicase (spot GL 8) were down-regulated. Thus, we suggest that these proteins might participate in the early response to salt stress in ginseng leaves.

Keywords

References

  1. Aghaei K and Komatsu S. (2013). Crop and medicinal plants proteomics in response to salt stress. Frontiers in Plant Science. 4:8. doi: 10.3389/fpls.2013.00008.
  2. Attele AS, Wu JA and Yuan CS. (1999). Ginseng pharmacology-Multiple constituents and multiple actions. Biochemical Pharmacology. 58:1685-1693. https://doi.org/10.1016/S0006-2952(99)00212-9
  3. Bang KH, Seo AY, Chung JW, Kim YC, Jo IH, Kim JU, Kim DH, Cha SW, Cho YG and Kim HS. (2012). Analysis of genetic polymorphism and relationship of korean ginseng cultivars and breeding lines using EST-SSR marker. Korean Journal of Medicinal Crop Science. 20:277-285. https://doi.org/10.7783/KJMCS.2012.20.4.277
  4. Cao WH, Liu J, He XJ, Mu RL, Zhou HL, Chen SY and Zhang JS. (2007). Modulation of ethylene responses affects plant salt-stress responses. Plant Physiology. 143:707-719.
  5. Fisher M, Gokhman I, Pick U and Zamir A. (1996). A salt-resistant plasma membrane carbonic anhydrase is induced by salt in Dunaliella salina. The Journal of Biological Chemistry. 271:17718-17723. https://doi.org/10.1074/jbc.271.30.17718
  6. Guo H and Ecker JT. (2004). The ethylene signaling pathway: new insights. Current Opinion in Plant Biology. 7:40-49. https://doi.org/10.1016/j.pbi.2003.11.011
  7. Jung HW, Lim CW, Lee SC, Choi HW, Hwang CH and Hwang BK. (2008). Distinct roles of the popper hypersensitive induced reaction protein gene CaHIR1 in disease and osmotic stress, as determined by comparative transcriptome and proteome analyses. Planta. 227:409-425.
  8. Jung JD, Park HW, Hahn Y, Hur CG, In DS, Chung HJ, Liu JR and Choi DW. (2003). Discovery of genes for ginsenoside biosynthesis by analysis of ginseng expressed sequence tag. Plant Cell Reports. 22:224-230. https://doi.org/10.1007/s00299-003-0678-6
  9. Kim GH, Seong BJ, Kim SI, Han SH, Kim HH and Lee KS. (2011). Yield and quality characteristics of ginseng's first byproducts. Korean Journal of Medicinal Crop Science. 19:313-318. https://doi.org/10.7783/KJMCS.2011.19.5.313
  10. Kim SI, Kim JY, Kim EA, Kwon KH, Kim KW, Cho K, Lee JH, Nam MH, Yang DC, Yoo JS and Park YM. (2003). Proteome analysis of hairy root from Panax ginseng C. A. Meyer using peptide fingerprinting, internal sequencing and expressed sequence tag data. Proteomics. 3:2379-2392. https://doi.org/10.1002/pmic.200300619
  11. Kim SI, Kweon SM, Kim EA, Kim JY, Kim S, Yoo JS and Park YM. (2004a). Characterization of RNase-like major storage protein from the ginseng root by proteomic approach. Journal of Plant Physiology. 161:837-845. https://doi.org/10.1016/j.jplph.2004.01.001
  12. Kim ST, Bae DW, Lee KH, Hwang JE, Bang KH, Kim YC, Kim OT, Yoo NH, Kang KY, Hyun DY and Lim CO. (2008). Proteomic analysis of Korean ginseng(Panax ginseng C. A. Meyer) following exposure to salt stress. Journal of Plant Biotechnology. 35:185-193. https://doi.org/10.5010/JPB.2008.35.3.185
  13. Kim ST, Kim SG, Agrawal GK, Kikuchi S and Rakwal R. (2014). Rice proteomics: A model system for crop improvement and food security. Proteomics. 14:4-5. https://doi.org/10.1002/pmic.201200563
  14. Kim ST, Kim SG, Hwang DH, Kang SY, Kim HJ, Lee BH, Lee JJ and Kang KY. (2004b). Proteomic analysis of pathogenresponsive proteins from rice leaves induced by rice blast fungus, Magnaporthe grisea. Proteomics. 4:3569-3578. https://doi.org/10.1002/pmic.200400999
  15. Kim UG, Jung HJ, Lee SJ and Kim ST. (2012). Crop proteomics: Practical method for high resolution of two-dimensional electrophoresis. Journal of Plant Biotechnology. 39:81-92. https://doi.org/10.5010/JPB.2012.39.1.081
  16. Kim YC, Kim DH, Bang KH, Kim JU, Hyun DY, Lee SW, Kang SW, Cha SW, Kim KH, Choi JK, Han SH, An YN and Jeong HN. (2013). A high yielding and salt resistance ginseng variety 'Cheonryang'. The Korean Journal of Breeding Science. 45:434-439. https://doi.org/10.9787/KJBS.2013.45.4.434
  17. Ma R, Sun LW, Chen XN, Jiang R, Sun H and Zhao DQ. (2013). Proteomic changes in different growth periods of ginseng roots. Plant Physiology and Biochemistry. 67:20-32. https://doi.org/10.1016/j.plaphy.2013.02.023
  18. Nagappan A, Karunanithi N, Sentrayaperumal S, Park KI, Park HS, Lee DH, Kang SR, Kim JA, Senthil K, Natesan S, Muthurajam R and Kim GS. (2012). Comparative root protein profiles of Korean ginseng(Panax ginseng) and indian ginseng(Withania sonifera). The American Journal of Chinese Medicine. 40:203-218. https://doi.org/10.1142/S0192415X12500164
  19. Nam MH, Kim SI, Liu JR, Yang DC, Lim YP, Kwon KH, Yoo JS and Park YM. (2005). Proteomic analysis of korean ginseng(Panax ginseng C. A. Meyer). Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Science. 815:147-155. https://doi.org/10.1016/j.jchromb.2004.10.063
  20. Pandey A and Mann M. (2000). Proteomics to study genes and genomes. Nature. 405:837-846. https://doi.org/10.1038/35015709
  21. Rabilloud T. (2002). Two-dimensional gel electrophoresis on proteomics: old, old fashioned, but it still climbs up the mountains. Proteomics. 2:3-10. https://doi.org/10.1002/1615-9861(200201)2:1<3::AID-PROT3>3.0.CO;2-R
  22. Sahoo RK, Gill SS and Tuteja N. (2012). Pea DNA helicase 45 promotes salinity stress tolerance in IR64 rice with improved yield. Plant Signaling & Behavior. 7:1042-1046. https://doi.org/10.4161/psb.20915
  23. Sun LW, Ma PT, Li DN, Lei XJ, Ma R and Qi C. (2011). Protein extraction from the stem of Panax ginseng C. A. Meyer: A tissue of lower protein extraction efficiency for proteomic analysis. African Journal of Biotechnology. 10:4328-4333.
  24. Takahashi D, Li B, Nakayama T, Kawamura Y and Uemura M. (2014). Shotgun proteomics of plant plasma membrane and microdomain proteins using nano-LC-MS/MS. Methods in Molecular Biology. 1072:481-498. https://doi.org/10.1007/978-1-62703-631-3_33
  25. We JS, Park HS and Kwon KR. (2007). Proteome analysis of various types of Panax ginseng using 2-dimensional electrophoresis. Journal of Pharmacopuncture. 10:5-18. https://doi.org/10.3831/KPI.2007.10.2.005

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