BioChip Journal

The Korean BioChip Society (한국바이오칩학회)
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Optimization of Microwell-based Cell Docking in Microvalve Integrated Microfluidic Device

  • Song, Kang (Department of Energy Science and Technology, Graduate School of Energy Science and Technology, Chungnam National University) ;
  • Jeong, Heon-Ho (Department of Chemical Engineering, Chungnam National University) ;
  • Jin, Si Hyung (Department of Chemical Engineering, Chungnam National University) ;
  • Park, Jin-Sung (School of Mechanical, Aerospace and Systems Engineering, Division of Mechanical Engineering, Korea Advanced Institute of Science and Technology) ;
  • Lee, Chang-Soo (Department of Energy Science and Technology, Graduate School of Energy Science and Technology, Chungnam National University)
  • Received : 2014.07.04
  • Accepted : 2014.09.02
  • Published : 2014.09.20
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Abstract

This study presents a novel cell docking system based on microwells integratded with microvalves. Conventional cell docking device based on micro-well suffers from generation of dead volume and shear stress within micro-wells resulting in low efficiency of cell docking, limitation of nutrient, and low cell viability. Our approach to solve the problems adopts integration of microvalve controlled by pressure with microwells for provinding guided flow stream of cells and nutrients into microwell. We have optimized the efficiency of cell docking by varying several experimental parameters including flow rate, cell concentration, microvalve pressure, and size of microvalve. Under the optimized flow rate ($1{\mu}L/sec$) and valve pressure (0.2 MPa), we obtain high efficiency of cell docking as 14.1 cells/microwell. In this study, we confirm that the perfusion culture of cells in microfluidic chip provides suitable environmental condition for cell culture at small scale and demonstrate the feasibility of universal cell culture platform.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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