Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Root of Taraxacum coreanum Nakai on the Inhibition of Inflammation and Oxidative Stress Induced by Lipopolysaccharide in ICR Mice

흰 민들레 뿌리의 항염증 및 산화 스트레스 개선 효과

  • Cho, Byung-Je (Chedam Hospital of Korean Medicine) ;
  • Kim, Mijeong (Department of Food and Nutrition, Silla University) ;
  • Song, Yeong Ok (Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University)
  • 조병제 (체담한방병원) ;
  • 김미정 (신라대학교 식품영양학과) ;
  • 송영옥 (부산대학교 식품영양학과 및 김치연구소)
  • Received : 2015.09.24
  • Accepted : 2015.11.18
  • Published : 2015.12.31
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Abstract

The effects of root of Taraxacum coreanum Nakai (TC), on the suppression of inflammation and oxidative stress induced by lipopolysaccharide (LPS) in ICR mice were studied. LPS (10 mg/kg body weight) was injected into ICR mice in between two consecutive oral administrations. Hot water extract of fresh TC (HWETC) was administered to mice immediately before and 24 h after LPS injection. The animal groups used in this study were as follows: NOR group (PBS injection, DW administration), CON group (LPS injection, DW administration), and TC group (LPS injection, 1.4 g/kg bw of HWETC administration). Mice in the CON group lost weight due to inflammation induced by LPS, while the body weight of the TC group mice increased significantly, indicating that inflammation was inhibited by HWETC administration. Compare with the CON group, plasma and hepatic triglyceride, reactive oxygen species, peroxynitrite, and hepatic thiobarbituric acid reactive substances concentrations of the TC group decreased significantly (P<0.05). The protein expression of a pro-inflammatory transcription factor, nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) and its target enzyme, cyclooxygenase 2, increased in response to LPS injection, but was suppressed by HWETC administration (P<0.05). In conclusion, HWETC appears to ameliorate the oxidative stress and inflammatory responses induced by LPS via inhibition of $NF-{\kappa}B$ activation.

한방에서 염증 치료 목적으로 사용하는 흰 민들레 뿌리의 열수 추출물을 제조하여 항염증 효과 및 산화 스트레스 개선효과를 확인하고자 하였다. ICR mice에 lipopolysaccharide(LPS)를 1회 복강 투여하여(10 mg/kg bw) 염증 및 산화스트레스를 유발하고, 한국에만 자생하는 흰 민들레 뿌리 열수 추출물을 1.4 g/kg bw 농도로 2회 경구 투여하였다. LPS에 의한 염증 유발은 체중 감소 현상으로 확인하였고, 민들레 뿌리 열수 추출물 투여군은 유의적으로 체중이 증가하여 염증 억제 효과가 관찰되었다. LPS 처리는 혈액의 중성지방질, 혈액 및 간의 reactive oxygen species(ROS), peroxynitrite 농도 그리고 간의 지질과산화 수준을 증가시켰다(P<0.05). LPS를 처리한 대조군에 비해 흰 민들레 뿌리 열수 추출물 투여군은 혈중 및 간의 중성지방질, ROS, peroxynitrite 농도 그리고 간의 지질과산화물 수준이 유의적으로 감소하였다(P<0.05). 이는 흰 민들레 뿌리 열수 추출물이 간의 $NF-{\kappa}B$ 전사인자 및 이의 조절을 받는 COX-2의 단백질 발현을 억제함으로써 염증 관련 매개체 및 유리기 생성을 유의적으로 억제하였기 때문으로 생각된다. LPS 처리군에서 간의 중성지방질 농도가 감소한 것은 염증에 의해 간 조직이 손상되어 중성지방질의 합성이 억제되었기 때문으로 생각된다. 본 연구는 한방에서 사용하여 온 한국 토종 흰 민들레 뿌리가 $NF-{\kappa}B$ 전사인자의 발현을 억제함으로써 염증 및 산화 스트레스를 개선하는 효과가 있음을 확인하였다.

Keywords

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