The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
권호 표지
DOI QR코드

DOI QR Code

Residual Characteristics of Bistrifluron and Fluopicolide in Korean Cabbage for Establishing Pre-Harvest Residue Limit

생산단계 잔류허용기준 설정을 위한 엇갈이배추 중 bistrifluron과 fluopicolide의 잔류 특성 연구

  • Lee, Dong Yeol (Divison of Applied Life Science(BK21 plus Program), Gyeongsang National University) ;
  • Jeong, Dong Kyu (Divison of Applied Life Science(BK21 plus Program), Gyeongsang National University) ;
  • Choi, Geun-Hyoung (Chemical Safety Division, National Institute of Agricultural Science, RDA) ;
  • Lee, Deuk-Yeong (Chemical Safety Division, National Institute of Agricultural Science, RDA) ;
  • Kang, Kyu Young (Divison of Applied Life Science(BK21 plus Program), Gyeongsang National University) ;
  • Kim, Jin Hyo (Institute of Agriculture and Life Science, Gyeongsang National University)
  • 이동열 (경상대학교 응용생명과학부(BK21플러스 농생명산업 차세대 인재육성사업단)) ;
  • 정동규 (경상대학교 응용생명과학부(BK21플러스 농생명산업 차세대 인재육성사업단)) ;
  • 최근형 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 이득영 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 강규영 (경상대학교 응용생명과학부(BK21플러스 농생명산업 차세대 인재육성사업단)) ;
  • 김진효 (경상대학교 농업생명과학연구원)
  • Received : 2015.09.23
  • Accepted : 2015.11.06
  • Published : 2015.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study investigated residual characteristics of bistrifluron and fluopicolide in Korean cabbage, and suggested the pre-harvest residue limits (PHRLs) based on their dissipation patterns and biological half-lives. The pesticides were sprayed on Korean cabbage in two different region under greenhouse conditions at the recommended dose, respectively. The samples for residue analysis were harvested at 0 (2 hr), 1, 2, 4, 6, 8 and 10 days after treatment, and analyzed by HPLC after clean-up with Florisil SPE. The limit of quantification (LOQ) was $0.03mg\;kg^{-1}$ for bistrifluron and fluopicolide, and the recoveries ranged from 87.2-110.6% with below 5% of RSD. The biological half-lives of field I and field II were 3.9 and 4.2 days for bistrifluron and 4.9 and 4.2 days for fluopicolide, respectively. The PHRL of bistrifluron and fluopicolide were recommended as 3.83 and $3.23mg\;kg^{-1}$ for 10 days before harvest, respectively.

본 연구는 엇갈이배추 중 bistrifluron과 fluopicolide의 잔류특성을 조사하여 약제별 잔류량 감소추이와 생물학적 반감기를 바탕으로 생산단계 잔류허용기준(pre-harvest residue limit, PHRL) 설정을 위한 기초자료로 활용하고자 수행되었다. 온실 재배 중인 엇갈이배추에 안전사용기준 농도로 희석한 bistrifluron과 fluopicolide를 다른 두 지역의 포장에 각각 살포 후 0 (2시간), 1, 2, 4, 6, 8, 10일에 시료를 채취하여 분석하였다. 엇갈이배추 중 두 성분의 잔류농약은 acetonitrile을 이용하여 추출하고 Florisil SPE cartridge로 정제한 후 HPLC/DAD를 이용하여 분석하였다. 두 시험농약의 정량한계는 $0.03mg\;kg^{-1}$이었고, 시험분석법의 회수율은 0.1 및 $0.5mg\;kg^{-1}$ 두 수준에서 87.2%~110.6%이었으며 상대표준편차는 모두 5% 미만이었다. 두 지역의 시험포장에서 엇갈이배추 중 각 시험농약의 생물학적 반감기는 bistrifluron의 경우 3.9일과 4.2일이었으며, fluopicolide의 경우 4.9일과 4.2일로 나타났다. 잔류회귀감소식을 이용하여 엇갈이배추 중 bistrifluron과 fluopicolide의 생산단계 잔류허용기준은 수확 10일 전 $3.83mg\;kg^{-1}$과 $3.23mg\;kg^{-1}$으로 제안하였다.

Keywords

References

  1. Anatassiades, M., S. J. Lehotay, D. Stajnbaher and F. J. Scheck (2003) Fast and easy multiresidue method employing acetonitrile extraction/partitioning and "dispersive solidphase extraction" for the determination of pesticide residues in produce, J. AOAC Int. 86(1):412-431.
  2. Bae, B. J., H. K. Lee, K. A. Son, G. J. Im, J. B. Kim, T. H Kim, S. Chae and J. W. Park (2012) The residue property of fungicide Boscalid and Fluidioxonil at the same time harvest leafy-vegetables. Korean J. Pestic. Sci. 16(2):98-108. https://doi.org/10.7585/kjps.2012.16.2.098
  3. Chang, H. S., H. R. Bae, Y. B. Son, I. H. Song, C. H. Lee, N. G. Choi, K. K. Cho and Y. G. Lee (2011) Developing a webbased system for computing pre-harvest residue limits (PHRLs). Agribusiness and Information Management. 3(1):11-37. https://doi.org/10.14771/AIM.3.1.2
  4. Fenik, J., M. Tankiewicz and M. Biziuk (2011) Properties and determination of pesticides in fruits and vegetables, Trac-Trends Anal. Chem. 30(6):814-826. https://doi.org/10.1016/j.trac.2011.02.008
  5. Jeong, Y. H., J. U. Kim, J. H. Kim, Y. D. Lee, C. H. Lim and J. H. Hur (2004) Modern pesticide. first edition, Sigma-press, Republic of Korea. pp.5.
  6. Jeon, S. O., J. I. Hwang, S. H. Lee and J. E. Kim (2014) Uptake of boscalid and chlorfenapyr residues in soil into Korean cabbage. Korean J. Pestic. Sci. 18(4):314-320. https://doi.org/10.7585/kjps.2014.18.4.314
  7. Kim, D. K., J. K. Kim, E. Y. Lee, I. Y. Park, H. H. Noh, Y. S. Park, T. H. Kim, C. W. Jin, K. I. Kim, S. S. Yun, S. K. Oh and K. S. Kyung (2007) Residual Characteristics of Some Pyrethroid Insecticides in Korean Cabbage. Korean J. Pestic. Sci. 11(3):154-163.
  8. Kim, Y. S., J. H. Park, J. W. Park, Y. D. Lee, K. S. Lee and J. E. Kim (2002) Persistence and dislodgeable residues of chlorpyrifos and procymidone in lettuce leaves under greenhouse condition. Korean J. Environ. Agri. 21(2):149-155. https://doi.org/10.5338/KJEA.2002.21.2.149
  9. Ko, G. Y., K. H. Kim and K. S. Lee (2004) Residual pattern of procymidone and chlorothalonil in grape during the period of cultivation and storage. Korean J. Environ. Agri. 23(1): 47-51. https://doi.org/10.5338/KJEA.2004.23.1.047
  10. Ko, G. Y., S. H. Kim, Y. H. Jang and K. S. Lee (2008) Residual pattern of chlorothalonil, indoxacarb, lufenuron, metalaxyl and methomyl during the cultivation periods in chinese cabbage, Korean J. Pestic. Sci. 12(1):34-42.
  11. Korea Crop Protection Association (2012) 2012 Guideline of Pesticide Use. Republic of Korea. pp.399, 514.
  12. Lee, D. Y., Y. J. Kim, M. H. Park, S. H. Lee, S. G. Kim, N. J. Kang and K. Y. Kang (2013) Establishment of pre-harvest residue limit (PHRL) of fungicides azoxystrobin and difenoconazole on prunus mume fruits, Korean J. Pestic. Sci. 17(4):307-313. https://doi.org/10.7585/kjps.2013.17.4.307
  13. Lee, D. Y., Y. J. Kim, S. J. Lee, K. S. Cho, S. G. Kim, M. H. Park and K. Y. Kang (2012) Establishment of pre-harvest residue limit of fungicide pyrimethanil and trifloxystrobin during cultivation of persimmon. Korean J. Environ. Agri. 31(1):45-51. https://doi.org/10.5338/KJEA.2012.31.1.45
  14. Lee, J. H., H. W. Park, Y. S. Keum, C. H. Kwon, Y. D. Lee and J. H. Kim (2008) Dissipation pattern of boscalid in cucumber under greenhouse condition, Korean J. Pestic. Sci. 12(1):67-73.
  15. Lee, Y. D (2011) Handbook for the pesticide residue analytical methods of Food Code Index. third edition, National Institute of Food and Drug Safety Evaluation, Republic of Korea. pp.80-82.
  16. Lehotay, S. J (2007) Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate: collaborative study, J. AOAC Int. 90(2):485-520.
  17. Marin, A., J. Oliva, C. Garcia, S. Navarro and A. Barba (2003) Dissipation rates of cyprodinil and fludioxonil in lettuce and table grape in the field and under cold storage conditions. J. Agric. Food Chem. 51(16):4708-4711. https://doi.org/10.1021/jf021222e
  18. Park, J. H., J. S. Park, A. M. Abd El-Aty, M. Rahman, T. W. Na and J. H. Shim (2012) Analysis of imidacloprid and pyrimethanil in shallot (Allium ascalonicum) grown under greenhouse conditions using tandem mass spectrometry: establishment of preharvest residue limits, Biomed. Chromatogr. 27(4):451-457. https://doi.org/10.1002/bmc.2812
  19. Tomlin, C. D. S. (2009) The Pesticide Manual, fifteenth edition, British Crop Production Council, UK, pp. 115-116, 533-534.

Cited by

  1. Establishment of Pre-Harvest Residue Limits (PHRL) of Flubendiamide and Pyriofenone on Strawberry (Fragaria ananassa Duch.) vol.21, pp.1, 2017, https://doi.org/10.7585/kjps.2017.21.1.62
  2. Pre-Harvest Residual Characteristics of Cyenopyrafen and Fluopicolide on Field-Grown Red Pepper vol.21, pp.2, 2017, https://doi.org/10.7585/kjps.2017.21.2.123
  3. Residual Characteristics of Pesticides Used for Powdery Mildew Control on Greenhouse Strawberry vol.52, pp.2, 2018, https://doi.org/10.14397/jals.2018.52.2.99
  4. 수박 중 및 Pyridalyl 및 Fluopicolide의 잔류 특성 및 생물학적 반감기 산출 vol.36, pp.1, 2015, https://doi.org/10.5338/kjea.2017.36.1.08
  5. Application of Kinetic Models for Residual Patterns of Chlorantraniliprole and Tetraconazole in Perilla leaves vol.23, pp.4, 2015, https://doi.org/10.7585/kjps.2019.23.4.297
  6. Residual Safety of Boscalid, Fluxapyroxad, Hexaconazole, Pencycuron, Pyraclostrobin, and Thifluzamide as Fungicides for the Prevention of Sclerotinia Rot on Carrot vol.25, pp.1, 2015, https://doi.org/10.7585/kjps.2021.25.1.11
  7. Residual Dissipation Pattern of Dichlorvos and Etofenprox in Squash under Greenhouse Condition vol.25, pp.1, 2015, https://doi.org/10.7585/kjps.2021.25.1.31