Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effects of White Habiscus syriacus L. Flower Extracts on Antioxidant Activity and Bone Resorption Inhibition

흰 무궁화 꽃 추출물의 항산화 활성과 골 흡수의 억제 효과

  • Lee, Hee Jung (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Sang Won (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Park, Chun Geun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Ahn, Young Sup (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Jin Seong (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Bang, Man Seok (Clinical Trial Institute, Biosafety & Validation Center, Dankook University) ;
  • Oh, Chung Hun (Clinical Trial Institute, Biosafety & Validation Center, Dankook University) ;
  • Kim, Chul Tae (Department of Emergency Medical Service, College Medical Science, Konyang University)
  • 이희정 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이상원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박춘근 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 안영섭 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김진성 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 방만석 (단국대학교 종합임상시험원, 바이오안전성 & 유효성 센터) ;
  • 오충훈 (단국대학교 종합임상시험원, 바이오안전성 & 유효성 센터) ;
  • 김철태 (건양대학교 의과학대학 응급구조학과)
  • Received : 2015.04.06
  • Accepted : 2015.05.30
  • Published : 2015.06.30
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Abstract

In this study, we tried to offer the possibility of White Hibiscus syriacus L. (WHS) flower extracts as a preventive and improving agent of osteoporosis that bone mass reduction is induced by an decrease of osteoblast involved in bone formation and increase of bone resorption by osteoclast activity. As a results, it was found to have antioxidant activity and contain a flavonoid contents ($47.74 mg/g) of the WHS flower. There was cytotoxicity at more than $250{\mu}g/m{\ell}$ concentration of WHS flower extract of RANKL-induced osteoclast in RAW264.7. There were no significant inhibited TRAP activity in the WHS leaf and stem. However, it was confirmed that it is significantly inhibited the differentiation activity of osteoclasts in 50 and $100{\mu}g/m{\ell}$ concentration of cells of stability levels of only WHS flower extracts (p < 0.01). The WHS flower prominently inhibited RANKL-induced osteoclast differentiation activity by decreased calcitonin receptor and TRAP mRNA (p < 0.01). These results indicate that of osteoclasts differentiation activity is inhibited by protection of oxidative stress due to the antioxidant activity of the WHS flower. Therefore, suggesting the WHS flower may be a presents the possibility as a preventive and therapeutic agents for osteoporosis.

Keywords

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