Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Antioxidant activity and hepatic lipids improvement effects of Rubus coreanus in high-fat diet-fed rats

복분자(Rubus coreanus) 추출물의 라디칼 소거활성 및 고지방 식이에 대한 흰쥐의 간 지질 개선 효과

  • Lee, Soo-Jung (Department of Food Science and Nutrition, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Song, Yuno (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Chung, Min-Sup (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Jang, Sun-Hee (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Won, Chung-kil (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Song, Young-Min (Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Cho, Jae-Hyeon (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
  • 이수정 (경상대학교 식품영양학과, 농업생명과학연구원) ;
  • 송윤오 (경상대학교 수의학과, 동물의학연구원) ;
  • 정민섭 (경상대학교 수의학과, 동물의학연구원) ;
  • 장선희 (경상대학교 수의학과, 동물의학연구원) ;
  • 원청길 (경상대학교 수의학과, 동물의학연구원) ;
  • 송영민 (경남과학기술대학교 동물소재공학과) ;
  • 조재현 (경상대학교 수의학과, 동물의학연구원)
  • Received : 2014.05.19
  • Accepted : 2014.06.10
  • Published : 2015.06.30
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Abstract

This study was investigated the effects on the radical scavenging activities and hepatic lipid levels in rats fed a high-fat diet (HFD) by the 70% ethanolic extract from Rubus coreanus. The Rubus coreanus extract (RCE) was administered through the gastrointestinal tract at 150 mg/kg BW/day for 5 weeks (HFD+RCE). Total phenol content was 157.93 mg gallic acid/g in the RCE. Scavenging activities to DPPH, ABTS, an d hydroxyl radicals by RCE significantly increased in a does-dependent manner of $50{\sim}500{\mu}g/mL$ concentration. DPPH and ABTS radical scavenging activities were over the 70% in the $500{\mu}g/mL$ concentration. During the five weeks study, the food efficiency ratio was no significant differences between the HFD and HFD+RCE. Liver weight of HFD+RCE (3.75 g/100 g BW) was significantly decreased compared to those of the HFD. Hepatic triglyceride and total cholesterol levels of HFD+RCE were significantly lower compared to those of the HFD. The hepatic TBARS contents in the HFD+RCE was significantly decreased to the HFD, radical scavenging activity was markedly increased in HFD+RCE as normal group. In histological observation of liver tissues, the hepatocytes of HFD+RCE appeared the reduced aspects on the size and numbers of lipid droplets in cytoplasm compared to the HFD. Our results suggest that RCE could have antioxidant activities and hepatic hypolipidemic effects by its phenolic compounds.

Keywords

References

  1. Ali KA, Abdelhalk M, George B, Panagiotis K. 2005. Tea and herbal infusions: Their antioxidant activity and phenolic propolis. Food Chem 89: 27-36. https://doi.org/10.1016/j.foodchem.2004.01.075
  2. Apel K, Hirt H. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology 55: 373-399. https://doi.org/10.1146/annurev.arplant.55.031903.141701
  3. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  4. Cho WG, Han SK, Sin JH, Lee JW. 2008. Antioxidant of heating pork and activities of Rubus coreanus Miq. extracts. J Korean Soc Food Sci Nutr 37: 820-825. https://doi.org/10.3746/jkfn.2008.37.7.820
  5. Cho YJ, Chun SS, Kwon HJ, Kim JH, Yoon SJ, Lee KH. 2005. Comparison of physiological activities between hot-water and ethanol extracts of Bokbunja (Rubus coreanum F.). J Korean Soc Food Sci Nutr 34: 790-796. https://doi.org/10.3746/jkfn.2005.34.6.790
  6. Choi HR, Lee JH, Lee SJ, Lee MJ, Jeong JT, Lee TB. 2014. Effects of unripe black raspberry water extract on lipid metabolism and oxidative stress in mice. Korean J Food Sci Technol 46: 489-497. https://doi.org/10.9721/KJFST.2014.46.4.489
  7. Choi HR, Lee SJ, Lee JH, Kwon JW, Lee HK, Jeong JT, Lee TB. 2013. Cholesterol-lowering effects of unripe black raspberry water extract. J Korean Soc Food Sci Nutr 42: 1899-1907. https://doi.org/10.3746/jkfn.2013.42.12.1899
  8. Gutfinger T. 1981. Polyphenols in olive oil. J Am Oil Chem Soc 58: 966-968. https://doi.org/10.1007/BF02659771
  9. Gutteridge JM. 1984. Reactivity of hydroxy and hydroxylike radicals discriminated by release of thiobarbituric acid reactive material from deoxy sugars, nucleosides and benzoate. J Biochem 224: 716-767.
  10. Hakkinen SH, Karenlampi SO, Heinonen IM, Mykkanen HM, Torronen AR. 1999. Content of the flavonols quercetin, myricetin and kaempferol in 25 edible berries. J Agric Food Chem 47: 2274-2279. https://doi.org/10.1021/jf9811065
  11. Hong SM, Kang MJ, Lee JH, Jeong JH, Kwon SH, Seo KI. 2012. Production of vinegar using Rubus coreanus and its antioxidant activities. Korean J Food Preserv 19: 594-603. https://doi.org/10.11002/kjfp.2012.19.4.594
  12. Hosseinian FS, Li W, Beta T. 2008. Measurement of anthocyanins and other phytochemicals in purple wheat. Food Chem 109: 916-924. https://doi.org/10.1016/j.foodchem.2007.12.083
  13. Hwang EK. 2009. Effect of quercetin supplement on major biochemical parameters in sera of rats fed high fat and high cholesterol diet. J Vet Clin 26: 413-418.
  14. Jang TS, Yang JC, Lim SY, Kim BA. 2014. Antioxidant and antihemolytic activity of ethanol extracts of Rubus coreanus Miquel. J Korean Oil Chemists' Soc 31: 130-135. https://doi.org/10.12925/jkocs.2014.31.1.130
  15. Jeong CH, Choi SG, Heo HJ. 2008. Analysis of nutritional compositions and antioxidative activities of Korean commercial blueberry and raspberry. J Korean Soc Food Sci Nutr 37: 1375-1381. https://doi.org/10.3746/jkfn.2008.37.11.1375
  16. Jung MA, Cho SH, Lee SY, Kim JH, Oh KN, Kim YS, Yoo GS, Lee DW, Kim SO. 2014. Effects of unripe Rubus coreanus Miquel extract on improvement of lipid metabolism in C57BL/6 mice fed a high-cholesterol diet. J Korean Soc Food Sci Nutr 43: 650-655. https://doi.org/10.3746/jkfn.2014.43.5.650
  17. Kammerer D, Claus A, Carle R, Schieber A. 2004. Polyphenol screening of pomace from red and white grape varieties (Vitis vinifera L.) by HPLC-DAD-MS/MS. J Agric Food Chem 52: 4360-4367. https://doi.org/10.1021/jf049613b
  18. Kang HH. 2009. Determination of biological activities of Korean berries and their anthocyanin identification. Ph.D. Thesis, Gyeongsang National University, Jinju, Korea.
  19. Kang SM, Shim JY, Hwang SJ, Hong SG, Jang HE, Park MH. 2003. Effects of Saengshik supplementation on health improvement in diet-induced hypercholesterolemic rats. J Korean Soc Food Sci Nutr 32: 906-912. https://doi.org/10.3746/jkfn.2003.32.6.906
  20. Kim HK, Kwon YA, Rho SK, Ham YT. 1999. The role of fatty acid binding protein in the fatty liver induced by alcohol or high cholesterol diet in rats. Kor J Nutr 32: 55-58.
  21. Kim ID, Kang SK, Kwon RH, Jeong OY, Lee JS, Ha BJ. 2007. The effect of Rubus coreanum Miquel against lipopolysaccharide-induced oxidative stress and lipid metabolism. J Fd Hyg Safety 22: 213-217.
  22. Kwon JW, Lee HK, Park HJ, Kwon TO, Choi HR, Song JY. 2011. Screening of biological activities to different ethanol extracts of Rubus coreanus Miq. Korean J Med Sci 19: 325-333.
  23. Lee BK, Shin HH. Jung JH. Hwang KT. Lee YS, Kim TY. 2009. Anthocyanins polyphenols and antioxidant activities of black raspberry exudates. J Korean Soc Food Sci Nutr 38: 125-130. https://doi.org/10.3746/jkfn.2009.38.2.125
  24. Lee JH, Lee SJ, Park S, Kim HK, Jeong WY, Choi JY, Sung NJ, Lee WS, Lim CS, Kim GS, Shin SC. 2011. Characterisation of flavonoids in Orostachys japonicus A. Berger using HPLC-MS/MS: contribution to the overall antioxidant effect. Food Chem 124: 1627-1633. https://doi.org/10.1016/j.foodchem.2010.08.031
  25. Lee SJ, Lee MJ, Ko YJ, Choi HR, Jeong JT, Choi KM, Cha JD, Hwang SM, Jung HK, Park JH, Lee TB. 2013. Effects of extracts of unripe black raspberry and red ginseng on cholesterol synthesis. Korean J Food Sci Technol 45: 628-635. https://doi.org/10.9721/KJFST.2013.45.5.628
  26. Lee SO, Kim MJ, Kim DG, Choi HJ. 2005. Antioxidative activities of temperature-stepwise water extracts from Inonotus obliquus. J Korean Soc Food Sci Nutr 34: 139-147. https://doi.org/10.3746/jkfn.2005.34.2.139
  27. Lim BO, Seo TW, Shin HM, Park DK, Kim SU, Cho KH, Kim HC. 2000. Effect of Betulae platyphyllae Cortex on free radical in diabetic rats induced by streptozotocin. Kor J Herbology 15: 69-77.
  28. Markakis P. 1974. Anthocyanins and their stability in foods. Crit Rev Food Technol 4: 437-456. https://doi.org/10.1080/10408397409527165
  29. Moreno MIN, Isla MI, Sanpietro AR, Vattuone MA. 2000. Comparison of the free radical scavenging activity of propolis from several region of Argentina. J Enthnopharmacol 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  30. Park JO, Lee IS, Choi JW. 2004. Effect of Terminalia chebula extract on liver in rat. J Life Sci 14: 141-147. https://doi.org/10.5352/JLS.2004.14.1.141
  31. Park SH, Jeon WK, Kim SH, Kim HJ, Park DI, Cho YK, Sung IK, Sohn CI, Keum DK, Kim BI. 2006. Prevalence and risk factors of non-alcoholic fatty liver disease among Korean adults. J Gastroenterol Hepatol 21: 138-143. https://doi.org/10.1111/j.1440-1746.2005.04086.x
  32. Park YG, Choi SH, Kim SH, Jang YS, Han JG, Chung HG. 2008. Functional composition and antioxidant activity from the fruits of Rubus coreanus according to cultivars. Mokche Konghak 36: 102-109.
  33. Qi Y, Zhao X, Lim YI, Park KY. 2013. Antioxidant and anticancer effects of edible and medicinal mushrooms. J Korean Soc Food Sci Nutr 42: 655-662. https://doi.org/10.3746/jkfn.2013.42.5.655
  34. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  35. Rhee SJ, Park HK. 1984. Change of lipid content and histochemical observation in liver of rats fed high fat diet. Korean J Nutr 17: 113-125.
  36. Song YO, Lee SJ, Park HJ, Jang SH, Chung BY, Song YM, Kim GS, Cho HJ. 2014. Hepatoprotective effect of Schisandra chinensis on high-fat diet- induced fatty liver in rats. Korean J Vet Serv 36: 45-52.
  37. Uchiyama M, Mihara M. 1978. Determination of malondialdehyde precursor in tissues by TBA test. Anal Biochem 86: 271-278. https://doi.org/10.1016/0003-2697(78)90342-1
  38. Wiseman H. 1996. Dietary influences on membrane function; important in protection against oxidative damage and disease. J Nutr Biochem 7: 2-6. https://doi.org/10.1016/0955-2863(95)00152-2
  39. Yoon HJ, Park YS. 2010. Effects of Scutellaria baicalensis water extract on lipid metabolism and antioxidant defense system in rats fed high fat diet. J Korean Soc Food Sci Nutr 39: 219-226. https://doi.org/10.3746/jkfn.2010.39.2.219
  40. Yoon I, Wee JH, Moon JH, Ahn TH, Park KH. 2003. Isolation and Identification of antioxidative activity from the fruits of Rubus coreanum Miquel. Korean J Food Sci Technol 35: 499-502.
  41. Zhang XH, Choi SK, Seo JS. 2010. Effect of dietary grape pomace on lipid metabolism and hepatic morphology in rats fed a high fat diet. J Korean Soc Food Sci Nutr 39: 1595-1603. https://doi.org/10.3746/jkfn.2010.39.11.1595

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