Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Antioxidant and Tyrosinase Inhibitory Effects of the Extract Mixtures of Perilla frutescens, Houttuynia cordata and Camellia sinensis

어성초, 자소엽, 녹차 식물 추출 혼합물의 항산화 및 Tyrosinase 저해 효과에 관한 연구

  • Lee, Kyung Eun (Department of Biotechnology, Yeungnam University) ;
  • Lee, Eun Sun (Department of Biotechnology, Yeungnam University) ;
  • Kang, Sang Gu (Department of Biotechnology, Yeungnam University)
  • 이경은 (영남대학교 생명공학부 생명공학전공) ;
  • 이은선 (영남대학교 생명공학부 생명공학전공) ;
  • 강상구 (영남대학교 생명공학부 생명공학전공)
  • Received : 2015.06.18
  • Accepted : 2015.06.22
  • Published : 2015.06.30
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Abstract

In the present study, antioxidant activities and tyrosinase inhibition of Perilla frutescens, Houttuynia cordata and Camellia sinensis extracts and the extract mixtures (PHC) were investigated. PHC showed 80.2% and 98.0% of free radical scavenging activity in DPPH and ABTS analysis, respectively, and 50% tyrosinase inhibition in $1000{\mu}g/mL$ concentration. HaCaT cells did not show cell toxicity in $100{\mu}g/mL$ of the PHC. Furthermore, HaCaT cell viability by co-culture with extract H. cordata was increased more than 10% compared with untreated cells. However, the cell viability was decreased in $500{\mu}g/mL$ of the extract C. sinensis and the PHC. These results suggested that about $100{\mu}g/mL$ concentration of the PHC showed proper tyrosinase inhibitory effect and antioxidant activities. The PHC could be used as multifunctional cosmeceutical agents.

본 연구에서는 항산화 기능을 가진 자소엽, 어성초 그리고 녹차 추출물들과 이들 추출물의 혼합물을 사용하여 항산화, 미백, 세포독성실험을 진행하였다. 자소엽, 어성초와 녹차의 추출물을 동일 양으로 혼합하였을 때 처리농도가 증가함에 따라 농도 의존적으로 DPPH와 ABTS 라디칼 소거활성이 높아졌으며, 추출물을 혼합한 경우 낮은 농도인 $10{\mu}g/mL$에서도 80.2%, 98.0%의 DPPH와 ABTS 라디칼을 소거시키는 우수한 항산화 효과를 나타내었다. 미백효과의 정도를 조사하기 위하여 tyrosinase 저해효과를 조사한 결과 자소엽과 녹차추출물 그리고 각 추출물의 혼합물이 농도 의존적으로 tyrosinase 저해효과가 증가되었다. HaCaT 세포를 사용한 세포독성실험의 경우 자소엽, 어성초 추출물의 경우 세포생존율이 무처리 대조군과 같거나 높게 나타났으며 특히 어성초추출물의 경우 $100{\mu}g/mL$에서 무처리 대조군 보다 10% 이상의 높은 생존율을 보였다. 녹차와 각 식물 추출물 혼합물의 경우 $500{\mu}g/mL$ 이상에서는 무처리 대조군에 비하여 세포생존율이 감소하였다. 그러므로 세 가지 식물의 추출물 혼합물이 $100{\mu}g/mL$ 농도일 때 세포에 안전하며 높은 항산화 활성과 함께 tyrosinase 저해 효과가 있었다.

Keywords

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