Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Garlic and Medicinal Plants Composites on Antioxidant Activity and Lipid Levels of Liver in Hypercholesterolemic Rats

마늘과 한약재 복합물의 항산화 활성 및 고콜레스테롤 급이 흰쥐의 간장 지질 함량에 미치는 영향

  • Lee, Soo-Jung (Dept. of Food Science and Nutrition, Gyeongsang National University) ;
  • Shin, Jung-Hye (Namhae Garlic Research Institute) ;
  • Kang, Min-Jung (Namhae Garlic Research Institute) ;
  • Yang, Seung-Mi (Dept. of Food Science and Nutrition, Gyeongsang National University) ;
  • Ju, Jong-Chan (Dept. of Hotel Curinary & Bakery,Changshin College) ;
  • Sung, Nak-Ju (Dept. of Food Science and Nutrition, Gyeongsang National University)
  • Published : 2009.12.30
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Abstract

The effects of combined garlic and medicinal plant extracts such as Gyeolmyeongja (Cassia obtusifolia Linne), Hasuo (Polygoni multiflori Radix), Youngji (Ganoderma lucium) and Sansayuk (Crataegus pinnatifida Bunge) on the antioxidant activity and lipid levels in the livers of rats fed a high cholesterol diet were analyzed. Total phenol and flavonoid contents were the highest in the Gyeolmyeongja by $151.02{\pm}5.20\;mg$/100 g and $43.69{\pm}5.58\;mg$/100 g. Electron donating ability, reducing power and hydroxyl radical scavenging activity were significantly increased when over 0.3% garlic extract was added. The antioxidant activity of linoleic acid in $\beta$- carotene increased in a dose dependant manner in response to the concentration of garlic extract. In livers of rats, the content of total lipids was significantly decreased by feeding garlic and medicinal plants composites; in particular, the group in which 0.7% garlic extract was added was the lowest. Total cholesterol was 14.95 mg/g in the control group; its level was lower in the groups fed garlic and medicinal plants composites, ranging from 11.47 to 11.86 mg/g. Triglyceride concentration was significantly decreased in the group fed 0.7% garlic extracts, with 46.42 mg/g compared to groups fed 0.3% and 0.5%. TBARS content showed a 15.8~17.6% decrease in groups fed 0.5~0.7% garlic extract and medicinal plants composites. Antioxidant activity was significantly increased in groups fed over 0.5% garlic extract compared to the control group. This study shows that garlic and medicinal plant composites intake is able to reduce the levels of liver lipids in hypercholesterolemic rats.

마늘과 한약재(결명자, 하수오, 영지 및 산사육) 복합물의 항산화능과 콜레스테롤을 급이한 흰쥐에 이를 급이하였을 때 간장 조직 중 지질 함량에 미치는 영향을 분석하였다. 한약재의 총 페놀 및 플라보노이드 함량은 151.02 mg/100 g 및 43.69 mg/g으로 결명자에서 가장 높았다. 마늘과 한약재 복합물의 전자공여능, 환원력 및 hydroxyl radical 소거능은 마늘 추출물 첨가량이 0.3% 이상일 때 첨가량에 따른 유의적인 차이를 보였다. $\beta$-carotene 존재 시 linoleic acid에 대한 항산화능은 마늘 추출물의 첨가량에 의존적으로 증가되었다. 콜레스테롤을 급이한 흰쥐에 마늘과 한약재 복합물을 급이하였을 때 총 지질의 함량은 대조군에 비해 유의적으로 감소되었으며, 마늘추출물의 0.7%첨가군(G-0.7%)에서 가장 낮았다. 총 콜레스테롤은 대조군에서는 14.95 mg/g이었는데, 마늘과 한약재 복합물 급이 후 11.47~11.86 mg/g으로 대조군에 비해 감소되었다. 중성지방은 마늘 추출물의 0.7% 첨가군에서 46.42 mg/g으로 0.3% 및 0.5%의 마늘 추출물 급이군에 비해 유의적으로 낮은 함량이었다. 마늘과 한약재 복합물 급이군에서 TBARS함량은 0.5~0.7%의 마늘 추출물 급이시 대조군에 비해 약 15.8~17.6% 감소되었다. 항산화능은 마늘 추출물을 0.5% 이상 첨가한 실험군에서 대조군에 비해 유의적으로 높았다. 본 실험결과로 볼 때 마늘과 한약재 복합물의 급이는 콜레스테롤급이 흰쥐의 간장조직의 지질 축적 저하에 효과적이라고 생각되어진다.

Keywords

References

  1. Basarkar, P. W. and N. Nath. 1981. Cholesterol lowering action of vitamin P-like compounds in rats. Indian J. Exp. BioI. 19, 787-789
  2. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 181, 1199-1200 https://doi.org/10.1038/1811199a0
  3. Chang, M. L. and M. A. Johnson. 1980. Effect of garlic on carbohydrate metabolism and lipid synthesis in rats. J. Nutr. 110, 931-936
  4. Choi, H. S., Y. H. Kim, J. H. Han, and S. H. Park. 2008. Effects of Eleutherococcus senticosus and several oriental medicinal herbs extracts on serum lipid concentrations. Korean J. Food & Nutr. 21, 210-217
  5. Cook, N. C. and S. Samman. 1996. Flavonoids-chemistry, metabolism, cardiovascular effects and dietary sources. Nutr. Biochem. 7, 66-76 https://doi.org/10.1016/0955-2863(95)00168-9
  6. Folch, J., M. Lees, and G. H. Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. BioI. Chem. 226, 497-502
  7. Fridovich, I. 1986. Biological effects of the superoxide radical. Adv. Enzymol. 58, 62-66
  8. Gebhardt, R. 1991. Inhibition of cholesterol biosynthesis by a water-soluble garlic extract in primary cultures of rat hepatocytes. Arzneimittelforschung 41, 800-804
  9. Gutfinger, T. 1958. Polyphenols in olive oil. J. Am. Oil. Chem. Soc. 58, 966-968
  10. Gutteridge, J. M. 1984. Reactivity of hydroxyl and hydroxyllike radicals discriminated by release of thiobarbituric acid reactive material from deoxy sugars, nucleosides and benzoate. Biochem. J. 224, 761-767 https://doi.org/10.3746/jkfn.2008.37.5.605
  11. Ha, T. Y., I. J. Cho, K S. Seong, and S. H. Lee. 2001. Effect of Cassia tora ethanol extract on the lipid levels of serum and liver in rats fed high cholesterol diet. J. Korean Soc. Food Sci. Nutr. 30, 1171-1176
  12. Haenen, G. R, J. B. Paquay, R E. Korthouwer, and A. Bast. 1997. Peroxynitrite scavenging by flavonoids. Biochem. Bioph. Res. Co. 236, 591-593 https://doi.org/10.1006/bbrc.1997.7016
  13. Ju, J. c., J. H. Shin, S. J. Lee, H. S. Cho, and N. J. Sung. 2006. Antioxidative activity of hot water extracts from medicinal plants. J. Korean Soc. Food Sci. Nutr. 35, 7-14 https://doi.org/10.3746/jkfn.2006.35.1.007
  14. Kang, J. A. and J. S. Kang. 1997. Effect of garlic and onion on plasma and liver cholesterol and triglyceride and platelet aggregation in rats fed basal or cholesterol supplemented diets. Korean J. Nutr. 30, 132-138
  15. Kang, M. J., S. J. Lee, J. H. Shin, S. K Kang, J. G. Kim, and N. J. Sung. 2008. Effect of garlic with different processing on lipid metabolism in 1 % cholesterol fed rats. J. Korean Soc. Food Sci. Nutr. 37, 162-169 https://doi.org/10.3746/jkfn.2008.37.2.162
  16. Kim, E. Y., I. H. Baik, J. H. Kim, S. R. Kim, and M. R Rhyu. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J. Food Sci. Technol. 36, 333-338
  17. Kim, H. K, K M. Na, S. H. Ye, and H. S. Han. 2004 . Extraction characteristics and antioxidative activity of Lycium chinense extracts. Korean J. Food Preserv. 11, 352-357
  18. Kim, S. Y., J. H. Kim, S. K Ki, M. J. Oh, and M. Y. Jung. 1994. Antioxidant activities of selected oriental herb extracts. J. Am. Oil Chem. Soc. 71, 633-640 https://doi.org/10.1007/BF02540592
  19. Koh, Y. J., D. S. Cha, H. D. Choi, Y. K Park, and I. W. Choi. 2008. Hot water extraction optimization of dandelion leaves to increase antioxidant activity. Korean J. Food Sci. Technol. 40, 283-289
  20. Lee, J. M., S. H Lee, and H M. Kim. 2000. Use of oriental herbs as medical food. Food Industry and Nutrition 5, 50-56
  21. Lee, S. E., N. S. Seong, J. K. Bang, C. G. Park, J. S. Seong, and J. Song. 2003. Antioxidative activity of Korean medicinal plant. Korean J. Med. Crop. Sci. 11, 127-134
  22. Lee, S. O., M. J. Kim, D. G. Kim, and H J. Choi. 2005. Antioxidative activities of temperature-stepwise water extracts from Inonotus obliquus. J. Korean Soc. Food Sci. Nutr. 34, 139-147 https://doi.org/10.3746/jkfn.2005.34.2.139
  23. Lee, Y. J. and Y. J. Son. 1999. The effects of Polygoni multiflori Radix and Cynanchi wilforgii Radix on the blood lipids and enzymes of hypercholesterolemic rats. Kor. J. Herbology 14, 69-77
  24. Lim, B. O., T. W. Seo, H. M. Shin, D. K. Park, S. U. Kim, K. H Cho, and H C. Kim. 2000. Effect of Betulae Platyphyllae Cortex on free radical in diabetic rats induced by streptozotocin. Kor. J. Herbology 15, 69-77
  25. Manian, R, N. Anusuya, P. Siddhyraju, and S. Manian. 2008. The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficus bengalensis L. and Ficus racemosa L. Food Chem. 107, 1000-1007 https://doi.org/10.1016/j.foodchem.2007.09.008
  26. Matsumoto, N., K. Okushio, and Y. Hara. 1998. Effect of black tea polyphenols on plasma lipids in cholesterol fed rats. J. Nutr. Sci. Vitaminol. 44, 337-342 https://doi.org/10.3177/jnsv.44.337
  27. Moreno, M. I. N., M. I. N. Isla, A. R Sampietro, and M. A. Vattuone. 2000. Comparison of the free radical scavenging activity of propolis from several region of Argentina. J. Enthropharmacol. 71, 109-114 https://doi.org/10.1016/S0378-8741(99)00189-0
  28. Na, G. M., H. S. Han, S. H Ye, and H K. Kim. 2004. Extraction characteristics and anti oxidative activity of Cassia ora L. extracts. Korean J. Food Culture 19, 499-505
  29. Nuttakaan, L., R Viboon, V. Nantaya, and M. G. Janusz. 2006. Quantitative evaluation of the antioxidant properties of garlic and shallot preparations. Nutrition 22, 266-274 https://doi.org/10.1016/j.nut.2005.05.010
  30. Oyaizu, M. 1986. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J. Nutr. 44, 307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  31. Saifer, A. and N. I. Feldman. 1971. The photometric determination of gangliosides with the sulfophospho-vanillin reaction. J. Lipid Res. 12, 112-115
  32. Shin, J. H, D. J. Choi, S. J. Lee, J. Y. Cha and N. J. Sung. 2008. Antioxidant activity of black garlic (Allium sativum L.). J. Korean Soc. Food Sci. Nutr. 37, 965-971 https://doi.org/10.3746/jkfn.2008.37.8.965
  33. Shin, J. H., S. J. Lee, J. K. Seo, I. S. Kim, S. K. Moon, and N. J. Sung. 2008. Effect of garlic hot-water extracts on the lipid metabolism in rats fed hyperlipidemic diet. The 49th International Symposium of Korean Society of Life Science. October 10-11. Daejeon, Korea
  34. Shin, S. Hand M. K. Kim. 2004. Effect of dried powders or ethanol extracts of garlic flesh and peel on antioxidative capacity in 16-month-old rats. Korean J. Nutr. 37, 633-644
  35. Srivastava, K. C., A. Bordia, and S. K. Verma. 1995. Garlic (Allium sativum) for disease prevention. South African J. Sci. 91, 68-77 https://doi.org/10.1016/S0952-3278(98)90034-5
  36. Summary of the second report of the national cholesterol education program (NCEP). 1993. Expert panel on detection, evaluation and treatment of high blood cholesterol in adults (Adult treatment panel II). JAMA 269, 3015-3023 https://doi.org/10.1001/jama.269.23.3015
  37. Taga, M. S., E. E. Miller, and D. E. Pratt. 1984. Chia seeds as a source of natural lipid antioxidant. J. Am. Oil Chem. Soc. 61, 928-931 https://doi.org/10.1007/BF02542169
  38. Uchiyama, M. and M. Mihara. 1978. Determination of malondialdehyde precursor in tissues by TBA test. Anal. Biochem. 86, 271-278 https://doi.org/10.1016/0003-2697(78)90342-1
  39. Yeh, Y. Y. and S. M. Yeh. 1994. Garlic reduces plasma lipids by inhibiting hepatic cholesterol and triacylglycerol synthesis. Lipids 29, 189-193 https://doi.org/10.1007/BF02536728
  40. Yugarani, T., B. K. H Tan, M. The, and N. P. Das. 1992. Effects of polyphenolic natural products on the lipid profiles of rats fed high fat diets. Lipids 27, 181-186 https://doi.org/10.1007/BF02536175

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