The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Identification of Wild Yeasts from Freshwaters and Soils of Nakdong and Yeongsan River, Korea, with Characterization of Two Unrecorded Yeasts

낙동강과 영산강 담수와 주변 토양으로부터 야생효모의 분리 및 동정

  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Kim, Ha-Kun (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Hyang-Burm (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 한상민 (배재대학교 바이오.의생명공학과) ;
  • 김하근 (배재대학교 바이오.의생명공학과) ;
  • 이향범 (전남대학교 응용생명공학부) ;
  • 이종수 (배재대학교 바이오.의생명공학과)
  • Received : 2016.09.19
  • Accepted : 2016.11.21
  • Published : 2016.12.30
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Abstract

Diverse wild yeast were isolated from freshwaters and soils of Nakdong and Yeongsan rivers in Korea and identified by the comparison of polymerase chain reaction-amplified nucleotide sequences of the internal transcribed spacer region (including the 5.8S rRNA) and D1/D2 regions of 26S rDNA, using BLAST. In total, 15 strains belonging to 9 species were isolated from 25 samples, out of which Aureobasidium pullulans and Cryptococcus bestiolae were dominant. Candida ghanaensis JSF0127 and Meira geulakonigii JSF0130 were identified as unrecorded yeasts, for which their mycological characteristics were investigated. These unrecorded yeasts formed ascospores and grew in yeast extract peptone dextrose medium containing 5% NaCl.

우리나라 주요 강 담수와 주변 토양 중의 야생효모 분포특성을 조사하고자 먼저 낙동강과 영산강 일부지역의 담수와 주변 토양 25점을 수집하여 15균주 9종의 야생효모들을 PCR을 이용한 ITS 부위와 26S rDNA의 D1/D2 부위의 염기서열 상동성 비교법을 이용하여 동정하였다. 이들 가운데 Aureobasidium pullulans와 Cryptococcus besiolae가 각각 3균주로 가장 많이 분리되었고, Lachancea thermotolerans와 Wickerhamomyces anomalus도 각각 2주씩 분리되었다. 특히 Candida ghanaensis와 Meira geulakonigii는 국내 처음 보고되는 미기록 균주들로서 포자를 형성하였고, YPD 배지와 potatoes dextrose 배지 등에서 잘 생육하는 특성을 갖고 있었다. 또한 두 미기록 효모들은 5% NaCl을 함유한 YPD 배지에서 생육하는 내염성 효모들이었다.

Keywords

References

  1. Lee JS, Yi SH, Kwon SJ, Ahn C, Yoo JY. Enzyme activities and physiological functionality of yeasts from traditional Meju. Kor J Appl Microbiol Biotechnol 1997;25:448-53.
  2. Kim JH, Lee DH, Jeong SC, Chung KS, Lee JS. Characterization of antihypertensive angiotensin I-converting enzyme inhibitor from Saccharomyces cerevisiae. J Microbiol Biotechnol 2004;14:1318-23.
  3. Jeong SC, Lee DH, Lee JS. Production and characterization of an anti-angiogenic agent from Saccharomyces cerevisiae K-7. J Microbiol Biotechnol 2006;16:1904-11.
  4. Min JH, Kim YH, Kim JH, Choi SY, Lee JS, Kim HK. Comparison of microbial diversity of Korean commercial Makgeolli showing high ${\beta}$-glucan content and high antihypertensive activity, respectively. Mycobiology 2012;40:138-41. https://doi.org/10.5941/MYCO.2012.40.2.138
  5. Jang IT, Kim YH, Yi SH, Lim SI, Lee JS. Screening of a new fibrinolytic substances-producing yeast. Kor J Mycol 2011;39:227-8. https://doi.org/10.4489/KJM.2010.39.3.227
  6. Lee DH, Lee DH, Lee JS. Characterization of a new antidementia ${\beta}$-secretase inhibitory peptide from Saccharomyces cerevisiae. Enzyme Microb Technol 2007;42:83-8. https://doi.org/10.1016/j.enzmictec.2007.08.003
  7. Lee DH, Lee JS, Yi SH, Lee JS. Production of the acetycholinesterase inhibitor from Yarrowia lipolytica S-3. Mycobiology 2008;36:102-5. https://doi.org/10.4489/MYCO.2008.36.2.102
  8. Jang IT, Kim YH, Kang MG, Yi SH, Lim SI, Lee JS. Production of tyrosinase inhibitor from Saccharomyces cerevisiae. Kor J Mycol 2012;40:60-4. https://doi.org/10.4489/KJM.2012.40.1.060
  9. Lee JS, Hyun KW, Jeong SC, Kim JH, Choi YJ, Miguez CB. Production of ribonucleotides by autolysis of Pichia anomala mutant and physiological activites. Can J Microbiol 2004;50:489-92. https://doi.org/10.1139/w04-032
  10. Min JH, Ryu JJ, Kim HK, Lee JS. Isolataion and identification of yeasts from wild flowers in Gyejoksan, Oseosan and Baekamsan of Korea. Kor J Mycol 2013;41:47-51. https://doi.org/10.4489/KJM.2013.41.1.47
  11. Hyun SH, Mun HY, Lee HB, Kim HK, Lee JS. Isolation of yeasts from wild flowers in Gyonggi-do province and Jeju island in Korea and the production of anti-gout xanthine oxidase inhibitor. Kor J Microbiol Biotechnol 2013;41:383-90. https://doi.org/10.4014/kjmb.1309.09002
  12. Hyun SH, Min JH, Lee HB, Kim HK, Lee JS. Isolation and diversity of yeasts from wild flowers in Ulleungdo and Yokjido, Korea. Kor J Mycol 2014;42:28-33. https://doi.org/10.4489/KJM.2014.42.1.28
  13. Hyun SH, Han SM, Lee JS. Isolation and physiological functionality of yeasts from wild flowers in Seonyudo of Gogunsanyeoldo, Jeollabuk-do, Korea. Kor J Mycol 2014;42:201-6. https://doi.org/10.4489/KJM.2014.42.3.201
  14. Han SM, Hyun SH, Lee JS. Isolation and identification of yeasts from wild flowers in Deogyu mountain and their physiological functionalities. Kor J Mycol 2015;43:47-52. https://doi.org/10.4489/KJM.2015.43.1.47
  15. Han SM, Hyun SH, Kim NM, Lee JS. Antioxidant activity and inhibitory activities of xanthine oxidase and tyrosinase of yeasts from wild flowers in Korea. Kor J Mycol 2015;43:99-103.
  16. Kim MC, Kang CK, Park HY, Lee DS, Kim YS, Lee WJ. Isotopic evidence of marine yeast to artificial cultural of Moina macrocopa. Kor J Microbiol 2006;42:111-5.
  17. Kim ML, Jeong JS, Lee GD. Change in growth of alcohol fermentation yeast with addition of deep seawater. Kor J Food Preserv 2003;10:417-20.
  18. Han SM, Hyun SH, Lee HB, Lee HW, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers collected around Jangseong lake in Jeollanam-do, republic of Korea, and characterization of the unrecorded yeast Bullera coprosmaensis. Mycobiology 2015;43:266-71. https://doi.org/10.5941/MYCO.2015.43.3.266
  19. Kaplow M. Commercial development of intermediate moisture foods. Food Technol 1970;24:889-93.
  20. Kurtzman CP. Four new Candida species from geographically diverse locations. Antonie Van Leeuwenhoek 2001;79:353-61. https://doi.org/10.1023/A:1012097023978
  21. Yasuda F, Yamagishi D, Izawa H, Kodama M, Otani H. Fruit strain of Japanese pear caused by basidiomycetous, yeastlike fungi Meira geulakonigii and Pseudozyma aphidis. Jpn J Phytopathol 2007;73:166-71. https://doi.org/10.3186/jjphytopath.73.166