Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Comparison of the antioxidant and physiological activities of grape seed extracts prepared with different drying methods

건조방법에 따른 포도씨의 항산화 활성의 변화

  • Jeong, Da-Som (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Youn, Kwang-Sup (Department of Food Science and Technology, Catholic University of Daegu)
  • 정다솜 (대구가톨릭대학교 식품공학전공) ;
  • 윤광섭 (대구가톨릭대학교 식품공학전공)
  • Received : 2015.11.05
  • Accepted : 2015.11.19
  • Published : 2016.02.28
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Abstract

The physiological activities of 70% ethanol extracts of grape seed (GS) prepared by freeze-drying (GSFD), infrared drying (GSIR), hot-air drying (GSHD), or sun-drying (GSSD) were investigated. The moisture contents of GSFD, GSIR, GSHD and GSSD powders were 4.53, 6.71, 6.91 and 5.55% respectively. Hunter's color value analysis revealed that the $L^*$ value of GSIR was lower, and the $a^*$ and $b^*$ values of GSIR were higher, than those of GSFD, GSHD, and GSSD. The total polyphenol and proanthocyanidin contents of GSFD were significantly higher than those of the other extracts. The flavonoid related substance contents were in the order of GSFD (7.68 g/100g) = GSSD (7.59 g/100g) = GSHD (7.33 g/100g) > GSIR (6.45 g/100g). The electron donating abilities of $500{\mu}g/mL$ solutions of GSFD, GSIR, GSHD and GSSD were 88.71, 52.62, 65.20, and 65.22%, respectively, while their reducing powers ($OD_{700}$) were 1.633, 1.097, 1.217 and 1.054 absorbance units, respectively. Additionally, the same trend was observed for the ABTS radical-scavenging abilities of the extracts as that observed for their electron-donating abilities and reducing powers. These results suggest that GSFD is the best method for preparing GS extracts with enhanced antioxidant activities, and that GS extracts may be used as a natural antioxidant material for use in health foods.

가공부산물로 버려지는 포도 부산물의 활용 및 생리활성 소재으로서의 개발 가능성을 알아보고자 건조 방법에 따른 포도씨 추출물에 대한 생리활성을 측정하였다. 열풍건조(6.91%)와 적외선건조(6.71%)가 가장 높았고, 색도는 적외선건조가 다른 건조방법에 비해 $L^*$ 값은 낮고 $a^*$ 값 및 $b^*$ 높은 경향을 나타내었다. 폴리페놀과 프로안토시아니딘 함량은 동결건조가 가장 높게 측정되었으며, 플라보노이드 함량은 건조방법 따른 유의적인 차이는 없었으나 동결건조가 가장 높은 함량을 나타내었다. $500{\mu}g/mL$의 농도에서 GSFD, GSIR, GSHD 및 GSSD 의 전자공여 활성은 각각 88.71%, 52.62%, 65.20% 및 65.22%로 동결건조가 다른 건조방법에 비하여 유의적으로 높은 활성을 나타내었고, 환원력과 ABTS 라디칼 소거활성에서도 전자공여 활성과 유사한 결과를 나타내었다. 이러한 결과를 종합해 볼 때 동결건조 포도씨 추출물이 적외선건조, 열풍건조 및 천일건조에 비해 소재 활용가치가 높을 것으로 사료되며, 천연항산화제 및 기능성 증진을 위한 소재로 이용 가능할 것으로 판단된다.

Keywords

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