Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Dual effect of curcumin on viability and motility of bovine sperm exposed to oxidative stress

산화스트레스에 노출된 정자의 생존성 및 운동성에 있어서 커큐민의 이중효과

  • Hwa, Jeong Seok (Department of Urology, College of Medicine, Gyeongsang National University) ;
  • Kim, Eun-Jin (Department of Physiology, College of Medicine, Gyeongsang National University) ;
  • Ryu, Ji Hyeon (Department of Physiology, College of Medicine, Gyeongsang National University) ;
  • Siregar, Adrian S. (Department of Physiology, College of Medicine, Gyeongsang National University) ;
  • Park, Chang Yoon (Department of Premedicine, College of Medicine, Gyeongsang National University) ;
  • Choe, Changyong (National Institute of Animal Sciences, Rural Development Administration) ;
  • Kang, Dawon (Department of Physiology, College of Medicine, Gyeongsang National University)
  • 화정석 (경상대학교 의과대학 비뇨기과학교실) ;
  • 김은진 (경상대학교 의과대학 생리학교실) ;
  • 류지현 (경상대학교 의과대학 생리학교실) ;
  • ;
  • 박창윤 (경상대학교 의과대학 의예과) ;
  • 최창용 (농촌진흥청 국립축산과학원) ;
  • 강다원 (경상대학교 의과대학 생리학교실)
  • Received : 2016.09.14
  • Accepted : 2016.09.29
  • Published : 2016.09.30
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Abstract

Although cryopreservation of sperm is routinely used for clinical requirement, it has some problems, such as high generation of reactive oxygen species (ROS) and cold-shock. To reduce the detrimental damage in sperm, anti-oxidants were added to cryoprotectant for sperm. Curcumin is one of anti-oxidants, which are added in cryoprotectants. However, recent studies have demonstrated that curcumin decreases sperm viability and motility. This study was performed to identify the effect of curcumin on hydrogen peroxide ($H_2O_2$)-exposed bovine sperm, which were cryopreserved-thawed. In $H_2O_2$-exposed bovine sperm, reactive oxygen species (ROS) were significantly reduced by treatment with curcumin in a dose-dependent manner (p < 0.05). Among tested concentrations of curcumin (1 to $50{\mu}M$), 30 and $50{\mu}M$ curcumin showed anti-oxidant effect on $H_2O_2$-induced ROS generation. On the other hand, combination of 30 or $50{\mu}M$ curcumin with anti-oxidant $H_2O_2$ increased the percentage of apoptotic sperm compared to only $H_2O_2$ treatment. Sperm viability was also decreased in the combination of 30 or $50{\mu}M$ curcumin with $H_2O_2$ as judged by FDA/PI staining. $H_2O_2$-induced decrease in sperm progressive motility was recovered by treatment with $1{\mu}M$ curcumin. These results show that high concentration of curcumin has anti-oxidant effect, but it has also cytotoxic effect on bovine sperm. Sperm viability and motility might be more affected by cytotoxic signals of curcumin compared to antioxidant signals.

Keywords

References

  1. Amidi F, Pazhohan A, Shabani Nashtaei M, Khodarahmian M, Nekoonam S. 2016. The role of antioxidants in sperm freezing: a review. Cell and Tissue Banking.
  2. Bielak-Zmijewska A, Sikora-Polaczek M, Nieznanski K, Mosieniak G, Kolano A, Maleszewski M, Styrna J, Sikora E. 2010. Curcumin disrupts meiotic and mitotic divisions via spindle impairment and inhibition of CDK1 activity. Cell Prolif. 43:354-364. https://doi.org/10.1111/j.1365-2184.2010.00684.x
  3. Bucak MN, Baspar N, Tuncer PB, Coyan K, Sarozkan S, Akaln PP, Buyukleblebici S, Kucukgunay S. 2012. Effects of curcumin and dithioerythritol on frozen-thawed bovine semen. Andrologia 44:102-109.
  4. Chen CC, Chan WH. 2012. Injurious effects of curcumin on maturation of mouse oocytes, fertilization and fetal development via apoptosis. Int J Mol Sci. 3:4655-4672.
  5. Gadea J, Selles E, Marco MA, Coy P, Matas C, Romar R, Ruiz S. 2004. Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology 62:690-701. https://doi.org/10.1016/j.theriogenology.2003.11.013
  6. Gough DR, Cotter TG. 2011. Hydrogen peroxide: a Jekyll and Hyde signalling molecule. Cell Death and Disease 2:e213. https://doi.org/10.1038/cddis.2011.96
  7. Duvoix, A., Blasius, R., Delhalle, S., Schnekenburger, M., Morceau, F., Henry, E., Dicato, M., and Diederich, M. 2005. Chemopreventive and therapeutic effects of curcumin. Cancer Lett. 223:181-190. https://doi.org/10.1016/j.canlet.2004.09.041
  8. Joe B., Vijaykumar, M., and Lokesh, B.R. 2004. Biological properties of curcumin-cellular and molecular mechanisms of action. Crit. Rev. Food Sci. Nutr. 44:97-111. https://doi.org/10.1080/10408690490424702
  9. Kumar A, Singh A. 2015. A review on mitochondrial restorative mechanism of antioxidants in Alzheimer's disease and other neurological conditions. Front Pharmacol. 6:206
  10. Kumar G, Mittal S, Sak K, Tuli HS. 2016. Molecular mechanisms underlying chemopreventive potential of curcumin: Current challenges and future perspectives. Life Sci. 148:313-328. https://doi.org/10.1016/j.lfs.2016.02.022
  11. Lim W, Jeong M, Bazer FW, Song G. 2016. Curcumin Suppresses Proliferation and Migration and Induces Apoptosis on Human Placental Choriocarcinoma Cells via ERK1/2 and SAPK/JNK MAPK Signaling Pathways. Biol Reprod.
  12. Mazzilli F1, Rossi T, Sabatini L, Pulcinelli FM, Rapone S, Dondero F, Gazzaniga PP. 1995. Human sperm cryopreservation and reactive oxygen species (ROS) production. Acta Eur Fertil. 26:145-148.
  13. Menon VP1, Sudheer AR. 2007. Antioxidant and anti-inflammatory properties of curcumin. Adv Exp Med Biol. 595:105-125.
  14. Naz RK. 2014. The Effect of Curcumin on Intracellular pH (pHi), Membrane Hyperpolarization and Sperm Motility. J Reprod Infertil. 15:62-70.
  15. Park BH, Lim JE, Jeon HG, Seo SI, Lee HM, Choi HY, Jeon SS, Jeong BC. 2016. Curcumin potentiates antitumor activity of cisplatin in bladder cancer cell lines via ROS-mediated activation of ERK1/2. Oncotarget.
  16. Sapanidou V, Taitzoglou I, Tsakmakidis Ι, Kourtzelis I, Fletouris D, Theodoridis A, Zervos I, Tsantarliotou M. 2015. Antioxidant effect of crocin on bovine sperm quality and in vitro fertilization. Theriogenology 84:1273-1282. https://doi.org/10.1016/j.theriogenology.2015.07.005
  17. Soleimanzadeh A, Saberivand A. 2013. Effect of curcumin on rat sperm morphology after the freeze-thawing process. Vet Res Forum. 4:185-189.
  18. Tejada S, Manayi A, Daglia M, Nabavi SF, Sureda A, Hajheydari Z, Gortzi O. Pazoki-Toroudi H, Nabavi SM. 2016. Wound Healing Effect of Curcumin: A Review. Curr Pharm Biotechnol.
  19. Tvrda E, Tusimova E, Kovacik A, Paal D, Greifova H, Abdramanov A, Lukac N. 2016. Curcumin has protective and antioxidant properties on bull spermatozoa subjected to induced oxidative stress. Anim Reprod Sci. 172:10-20. https://doi.org/10.1016/j.anireprosci.2016.06.008
  20. Wilken R, Veena MS, Wang MB, Srivatsan ES. 2011. Curcumin: A review of anti-cancer properties and therapeutic activity in head and neck squamous cell carcinoma. Mol Cancer. 10:12. https://doi.org/10.1186/1476-4598-10-12
  21. 전예진.김대영. 2013. 미니돼지 정자의 동결보존에 미치는 Curcumin의 항산화 효과. J. Chitin Chitosan. 18:190-197