Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Chemical Compositions and Antioxidant Activities of Essential Oil Extracted from Neolitsea aciculata (Blume) Koidz Leaves

새덕이 잎 정유의 성분분석 및 항산화활성 평가

  • Jeong, Min-Ji (Division of Wood Chemistry & Microbiology, Department of Forest Resources Utilization, National Institute of Forest Science) ;
  • Yang, Jiyoon (Division of Wood Chemistry & Microbiology, Department of Forest Resources Utilization, National Institute of Forest Science) ;
  • Choi, Won-Sil (National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Kim, Jae-Woo (Division of Wood Chemistry & Microbiology, Department of Forest Resources Utilization, National Institute of Forest Science) ;
  • Kim, Seok Ju (Division of Wood Chemistry & Microbiology, Department of Forest Resources Utilization, National Institute of Forest Science) ;
  • Park, Mi-Jin (Division of Wood Chemistry & Microbiology, Department of Forest Resources Utilization, National Institute of Forest Science)
  • 정민지 (국립산림과학원 임산공학부 화학미생물과) ;
  • 양지윤 (국립산림과학원 임산공학부 화학미생물과) ;
  • 최원실 (서울대 NICEM) ;
  • 김재우 (국립산림과학원 임산공학부 화학미생물과) ;
  • 김석주 (국립산림과학원 임산공학부 화학미생물과) ;
  • 박미진 (국립산림과학원 임산공학부 화학미생물과)
  • Received : 2016.12.09
  • Accepted : 2016.12.30
  • Published : 2017.01.25
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Abstract

This study aims to identify the chemical compositions and antioxidant activities of essential oil, extracted from the leaves of Neolitsea aciculata (Blume) Koidz by the hydrodistillation method. To the end, the chemical composition of N. aciculata leaf oil was firstly analyzed through GC/MS. The major constituents of essential oil were found to be: cis-ocimene (11.00%), trans-ocimene (9.65%), elemol (9.15%), ${\beta}$-elemene (8.75%), germacrene-D (7.55%), trans-caryophyllene (5.90%), ${\gamma}$-elemene (5.40%) and ${\tau}$-muurolol (4.95%). Then, the antioxidant potential of the essential oil was evaluated by the methods of total polyphenolic content (TPC) assay, DPPH radical scavenging activity, ABTS radical scavenging activity and ferric reducing antioxidant power (FRAP). It was estimated that the total polyphenolic content of the oil was $136.7{\pm}0.13\;mg\;GAE/g$ and the efficient concentration of the oil required to scavenge 50% DPPH radicals ($EC_{50}$ value) was $639.33\;{\mu}g/m{\ell}$. Also, ABTS radical scavenging activity was identified to be concentration dependent, while the FRAP value was $31.21{\pm}0.12\;{\mu}M$ $FeSO_4{\cdot}7H_2O/g$. Such figures, as a result, suggest that the essential oil extracted from the leaves of N. aciculata has its antioxidant activity, which can serve as significant health functional benefits.

본 연구는 hydrodistillation 방법으로 추출한 새덕이 잎 정유의 화학적 조성과 항산화활성을 조사하였다. 새덕이 잎 정유의 화학적 조성은 GC/MS로 분석하였다. 새덕이 잎 정유의 주요 구성성분은 cis-ocimene (11.00%), trans-ocimene (9.65%), elemol (9.15%), ${\beta}$-elemene (8.75%), germacrene-D (7.55%), trans-caryophyllene (5.90%), ${\gamma}$-elemene (5.40%), ${\tau}$-muurolol (4.95%)인 것으로 나타났다. 새덕이 잎 정유의 항산화 활성은 총 페놀함량 분석, DPPH radical 소거활성, ABTS radical 소거활성, ferric reducing antioxidant power (FRAP) 분석을 통해 평가하였다. 정유의 총 페놀 함량은 $136.7{\pm}0.13\;mg\;GAE/g$으로 분석되었으며, 50% DPPH radical을 소거하는데 필요한 정유의 효과 농도($EC_{50}$ value)는 $639.33\;{\mu}g/m{\ell}$로 나타났다. 또한, ABTS radical 소거활성은 농도 의존적으로 나타났으며, FRAP value는 $31.21{\pm}0.12{\mu}M$ $FeSO_4{\cdot}7H_2O/g$이었다. 이러한 결과는 새덕이 잎 정유가 건강에 대해 중요한 이점과 기능을 가질 수 있는 항산화 활성을 가지고 있다는 것을 제안한다.

Keywords

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