The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Caspase-dependent and Akt-pathway Regulated Rhus Verniciflua Stokes Extract-induced Apoptosis in Human Breast Carcinoma MDA-MB-231 Cells

인체 유방암세포 MDA-MB-231에서 건칠(乾漆) 추출물이 PI3K/AKT 신호경로를 통한 caspase 의존적 apoptosis 유발에 미치는 영향

  • Hong, Sang-hoon (Dept. of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University) ;
  • Park, Sang-eun (Dept. of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University)
  • 홍상훈 (동의대학교 한의과대학 한방내과) ;
  • 박상은 (동의대학교 한의과대학 한방내과)
  • Received : 2017.09.02
  • Accepted : 2017.09.25
  • Published : 2017.09.30
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Abstract

In Korea, Rhus verniciflua Stokes is used to purge hardness, alleviate blood stasis, and treat cancer. However, the mechanisms of related anti-cancer activity are not fully understood in human cancer cells. This study investigated the anti-cancer effects and mechanisms of Rhus verniciflua Stokes on MDA-MB-231 human breast cancer cells and found that treatment with a Rhus verniciflua Stokes extract resulted in time- and concentration-responses that indicated growth inhibition of breast cancer cells by induced apoptosis. This was followed by a decrease in mitochondrial membrane potential; the activation of caspase-3, -8, and -9; and the up-regulation of tBid. Caspase-dependent apoptosis was induced through the inhibition of phosphatidylinositol 3-kinase (PI3K) and the Akt signaling pathway. This study provides evidence that Rhus verniciflua Stokes might be useful for the treatment of breast cancer.

Keywords

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