Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Inhibitory Effects of Zerumbone on MCP-1-Induced THP-1 Migration

MCP-1에 의해 유도된 THP-1 유주에 미치는 Zerumbone의 영향

  • Kim, Sa Hyun (Department of Clinical Laboratory Science, Semyung University) ;
  • Kim, Si Hyun (Department of Clinical Laboratory Science, Semyung University) ;
  • Ryu, Sung Ryul (Department of Clinical Laboratory Science, Semyung University) ;
  • Lee, Pyeongjae (Department of Natural Medicine Resources, Semyung University) ;
  • Moon, Cheol (Department of Clinical Laboratory Science, Semyung University)
  • 김사현 (세명대학교 임상병리학과) ;
  • 김시현 (세명대학교 임상병리학과) ;
  • 유성률 (세명대학교 임상병리학과) ;
  • 이평재 (세명대학교 자연약재학과) ;
  • 문철 (세명대학교 임상병리학과)
  • Received : 2018.05.09
  • Accepted : 2018.05.22
  • Published : 2018.06.30
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Abstract

This study examined the effects of zerumbone on monocyte migration. Monocytes are recognized as important mediators of various inflammatory diseases, and the possibility of controlling inflammatory diseases by regulating the monocyte functions, such as activity and mobility, has been reported. MCP-1, which is a chemokine with levels that increase upon inflammation, causes the migration of the monocyte cell line, THP-1. Migration occurred at a concentration of 10 ng/mL MCP-1, and the highest migration occurred at 100 ng/mL and 200 ng/mL. MCP-1-induced THP-1 migration decreased by more than 40% in the presence of zerumbone. The concentration of cAMP, an important secondary messenger of the CCR2 signaling pathway, the MCP-1 receptor, was increased in the culture medium after a zerumbone treatment. The concentrations of cAMP decreased significantly under the MCP-1 treatment condition only. On the other hand, an increase in cAMP was observed when zerumbone and MCP-1 were treated simultaneously. Erk phosphorylation induced by an MCP-1 treatment was also found to decrease with the zerumbone treatment. This study introduces the possibility of controlling inflammatory diseases through the function of zerumbone, which regulates the migration of monocytes.

본 연구는 zerumbone이 단구의 유주에 어떠한 영향을 미치는지 알아보고자 진행되었다. 단구는 다양한 염증 질환의 중요한 매개자로 인식되고 있으며, 활성, 유주 등 단구의 기능 조절을 통해 염증 질환을 조절하는 가능성이 보고 되고 있다. 염증 발생 시 증가하는 케모카인인 MCP-1에 의해 단핵구 세포주 THP-1의 유주가 유발되는 것을 확인하였다. 10 ng/mL의 농도에서 유주가 발생하였으며, 100 ng/mL과 200 ng/mL의 농도에서 가장 높은 유주 현상이 나타났다. MCP-1에 의해 유발된 THP-1 유주는 zerumbone 존재 시 50% 이상 감소하였다. MCP-1 수용체인 CCR2 신호전달 과정의 중요 2차 전달자인 cAMP의 배양액 내 농도는 zerumbone 단독 처리 시 세포 단독 배양 조건에 비해 증가하였으며, MCP-1 단독 처리 시에는 의미있게 감소하였다. 그러나, zerumbone과 MCP-1을 동시에 처리했을 때에는 다시 cAMP의 증가가 관찰되었다. MCP-1 처리에 의해 일어나는 Erk 인산화도 zerumbone과 동시 처리 시 감소하는 결과를 확인했다. 본 연구는 염증성 질환에 중요한 매개자로 인식되고 있는 단구의 유주 현상을 조절하는 zerumbone의 가능성을 보여준다.

Keywords

References

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