Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Quantitative analysis of water-soluble vitamins and polyphenolic compounds in tomato varieties (Solanum lycopersicum L.)

토마토(Solanum lycopersicum L.) 품종 간 수용성 비타민과 폴리페놀계 성분 함량 변이 분석

  • Kim, Daen (Department of Horticultural Bioscience, Pusan National University) ;
  • Son, Beunggu (Department of Horticultural Bioscience, Pusan National University) ;
  • Choi, Youngwhan (Department of Horticultural Bioscience, Pusan National University) ;
  • Kang, Jumsoon (Department of Horticultural Bioscience, Pusan National University) ;
  • Lee, Yongjae (Department of Horticultural Bioscience, Pusan National University) ;
  • Je, Beungil (Department of Horticultural Bioscience, Pusan National University) ;
  • Park, Younghoon (Department of Horticultural Bioscience, Pusan National University)
  • 김다은 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 손병구 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 최영환 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 강점순 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 이용재 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 제병일 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 박영훈 (부산대학교 생명자원과학대학 원예생명과학과)
  • Received : 2020.01.13
  • Accepted : 2020.01.23
  • Published : 2020.03.31
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Abstract

Tomato fruit quality is determined by the contents of various functional metabolites in addition to fruit appearance. To develop tomato cultivars with higher amounts of functional compounds, an efficient quantification method is required to identify the natural variations in the compounds in the tomato germplasm. In this study, we investigated tomato varieties, which included 23 inbred lines and 12 commercial F1 cultivars, for their contents of seven watersoluble vitamins (vitamin C, vitamins B1, B2, B3, B5, B6, and B9) and five polyphenolic compounds (quercetin, rutin, kaempferol, myricetin, and naringenin chalcone). The results of high performance liquid chromatography and liquid chromatography-mass spectrometry showed that vitamin C and naringenin chalcone were the major water-soluble vitamins and polyphenolic compounds, respectively, and their abundance was highly variable depending on the cultivar. By contrast, the contents of vitamin B1, quercetin, and kaempferol were lowest among the cultivars. With regard to the relationship between metabolic compounds and fruit characteristics, a significant association was found in fruit size, indicating that cherry tomato varieties contain higher amounts of the compounds compared to large fresh-type varieties. However, no direct association was detected in fruit color, except for naringenin chalcone. The results of this study provide new insights on the quantification of metabolic compounds and the selection of breeding materials, which are prerequisites for the development of functional tomato varieties.

기능성 성분이 향상된 토마토 품종 개발을 위해서는 성분 정량분석법과 토마토 유전자원 간 대사성분 변이에 대한 정보의 확보가 필요하다. 본 연구에서는 토마토 유전자원23개 계통과 12개 상용 F1 품종을 이용하여 수용성 비타민 7종(vitamin C, B1, B2, B3, B5, B6, B9)과 폴리페놀계 성분 5종(quercetin, rutin, kaempferol, myricetin, and naringenin chalcone)에 대한 함량을 비교 분석 하였다. HPLC와 LC-MS 분석 결과, 수용성 비타민과 폴리페놀계의 주요 성분으로 vitamin C와 naringenin chalcone이 각각 검출되었으며 품종 간 높은 수준의 함량 변이가 존재함을 알 수 있었다. 반면에 vitamin B1, quercetin 과 kaempferol은 전 품종에 있어 함량이 가장 낮았다. 대사성분 함량과 토마토 과실특성 간 상관관계에 있어 서 과크기(과중)와 높은 유의성이 관찰되었는데 대부분의 성분에 있어 방울토마토 품종이 완숙용 토마토 품종보다 높은 함량을 보였다. 하지만 naringenin chalcone을 제외하고 대사성분과 과색 간의 상관관계는 뚜렷하게 나타나지 않았다. 본 결과는 토마토 육종과정에 활용될 수 있는 효율적인 대사성분 정량분석법을 제시할 뿐만 아니라 기능성 성분 고함량 육종소재 선발에 중요한 정보를 제공한다.

Keywords

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