Journal of Convergence for Information Technology (융합정보논문지)

Convergence Society for SMB (중소기업융합학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-oxidant, anti-inflammatory and Whitening effect of Benincasa hispida seed extract

동과자 추출물의 항산화, 항염 및 미백 효능

  • Park, Gyu-Ri (Dept. of Beauty Art, Graduate School, Seokyeong University) ;
  • Lee, Ji-An (Dept. of Beauty Art, Graduate School, Seokyeong University)
  • 박규리 (서경대학교 일반대학원 미용예술학과) ;
  • 이지안 (서경대학교 일반대학원 미용예술학과)
  • Received : 2020.05.11
  • Accepted : 2020.07.20
  • Published : 2020.07.28
Download PDF
⟨ Previous Next ⟩

Abstract

To investigate the natural cosmetic ingredients of Benincasa hispida seed extract on skin care, we measured anti-oxidant and anti-inflammatory, and whitening effect. DPPH free radical scavenging activity was increased in a dose-dependent manner. The total phenolic content was higher in methanol extract (22.42±0.002 mgGAE/g) than water extract (9.77±0.002 mgGAE/g). MTT assay was demonstrated that the seed extract did not have a cytotoxic effect in RAW264.7 and B16F10 cell lines. We also examined to find out the inhibitory activity on NO production and secretion of TNF-α cytokine in LPS-induced RAW264.7 cells. In B16F10 melanoma cells, the seed extract significantly suppressed α-MSH induced melanin synthesis. Furthermore, westernblot analysis revealed that methanol extract dramatically downregulated the expression level of MITF, TRP-1 and TRP-2. Taken together, the B. hispida seed extract posses anti-oxidant, anti-inflammatory and skin whitening activities, which might provided its functional efficacy in cosmetic materials.

본 연구는 피부 관리에 사용되는 천연화장품 원료로서 동과자 추출물의 활용 가능성을 조사하기 위해 항산화, 항염 그리고 미백 효능을 평가하였다. DPPH 자유라디컬 소거 활성은 농도 의존적으로 감소하였으며, 총폴리페놀 함량은 메탄올추출물(22.42±0.002 mgGAE/g)이 열수추출물(9.77±0.002 mgGAE/g)보다 더 높게 나타났다. MTT assay 결과 동과자 추출물에 의한 RAW264.7 세포와 B16F10 세포에서의 세포독성은 없었다. 또한 LPS 처리에 의해 활성화된 RAW264.7 세포에서 NO 생성과 TNF-α 분비 억제능을 확인하였다. B16F10 melanoma 세포에서 동과자 추출물은 α-MSH에 의한 멜라닌 합성을 유의하게 저해하는 것으로 나타났다. 게다가, westernblot 분석결과 동과자 메탄올추출물은 MITF, TRP-1 및 TRP-2 단백질의 발현 수준을 현저하게 감소시켰다. 이상의 결과로 동과자 추출물은 항산화, 항염 그리고 미백 효능을 가진 기능성 화장품 소재로써의 가치가 높을 것으로 사료된다.

Keywords

References

  1. N. S. Kim, K. C. Kim & W. Y. Oh. (2019) Study of Effects of dioscorea japonica Thunb Ethanol Extract on Anti-inflammation and Anti-wrinkle. Journal of the Korean Society of Cosmetology, 25(4), 947-954
  2. M. J. Kim, S. Y. Kim, K. H. Hym, D. S. Kim, S. Y. Kim & C. G. Hyun. (2017). Effects of Rumex axetosella, Sonchus oleraceus and Euphoibia jolkini Extracts on Melanin Synthesis in Melanoma Cells. Korean Society for Biotechnology and Bioengineering Journal 32(3), 187-19. DOI : 10.7841/ksbbj.2017.32.3.187
  3. K. N. Min. G. H. Lee, S. J. Park & T. B. Choe. (2019). Physiological activity and efficacy of cosmetic products in bio-converted soybean embryo extract. Journal of the Korea Covergence Society, 10(3), 211-220. DOI : 10.15207/JKCS.2019.10.3.211
  4. A. E. Al-Snafi. (2013). The Pharmacological Importance of Benincasa hispida. A review. International Journal of Pharma Sciences and Research, 4(2), 165-170.
  5. The Korean Pharmaceutical Association (1995), Korea professor conference, Phytology. (pp. 352-354). Seoul.
  6. Y. J. Kim & M. G. Shin. (1999). Mucolytic effects of various parts of Fructus Benincasae extracts in the rat trachea. The Journal Korean Medicine. 20(2), 165-176.
  7. J. K. Grover, G. Adiga, V. Vats & S. S. Rathi. (2001). Extracts of Benincasa hispida prevent development of experimental ulcers. Journal of Ethnophamacology. 78, 159-164. DOI : 10.1016/S0378-8741(01)00334-8
  8. H. R. Choi, K. H. Lee & C. H. Kim. (2003). Radiosensitizing and Antitumor Effect of the Seed of Benincasa hispida. Korean Journal of Food Science and Technology. 35(3), 479-482.
  9. S. Rana & A. Suttee. (2012). Phytochemical Investigation and Evaluation of Free Radical Scavenging Potential of Benincasa hispida Peel Extracts. International Journal of Current Pharmaceutical Review and Research, 3(3), 43-46.
  10. M. S. Blios. (1958). Antioxidant determinations by the use of a stable free radical, Nature, 181, 1199-1200. https://doi.org/10.1038/1811199a0
  11. AOAC. (1980). Official Methods of Analysis. 13 th ed., Association of Official Analytical Chemists., Washington D.C., pp.376-384 DOI : 10.1002/jps.2600700437
  12. Y. Yang & J. A. Lee. (2019). Antioxidant and Anti-inflammatory Effect of Pyracantha Angustifolia Fruit Extracts. Journal of Convergence for Information Technology, 9(12), 294-301. DOI : 10.22156/CS4SMB.2019.9.12.294
  13. H. Lee. (2019). Skin lightening effect of fermented Panax ginseng extract. Journal of the Korea Covergence Society, 10(2), 285-292. DOI : 10.15207/JKCS.2019.10.2.285
  14. J. Hosoi, E. Abe, T. Suda & T. Kuroki. (1985). Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-Dihydroxyvitamin D3 and retinoic acid. Cancer Research, 45(4), 1474-1478.
  15. E. S. Kim, J. Y. Choi, S. J. Hwang & I. H. Bae. (2019). Hypermethylation of miR-205-5p by IR Govern Aggressiveness and Metastasis via Regulating Bcl-w and Src. Molecular Therapy Nucleic Acids, 14, 450-464. DOI : 10.1016/j.omtn.2018.12.013
  16. N. Abdullah, W. S. Kamarudin, Z, Samicho, N. Azima & K. Zulkifli. (2012). Evaluation of In-Vitro Antioxidant and Antimicrobial Activities of the Various Parts of Benincasa hispida. International Journal of PharmTech Research, 4(4), 1367-1376.
  17. B. Mandana, A. R. Russly, S. T. Farah, M. A. Noranizan, I. S. Zaidul & G. Ali. (2012) Antioxidant activity of winter melon (Benincasa Hispida) seeds using conventional soxhlet extraction technique. International Food research Journal, 19(1), 229-234.
  18. S. Y. kim, K. H. Park, H. D. Moon, S. H. Jung, S. E. Lee & B. H. Kim. (2017). Anti-Inlfammtory Effects of acai Berry Ethanol Extracts. Journal of the Korean Society of Cosmetology, 23(4), 669-676.
  19. N. S. Gill, K. Dhiman, P. Sharma & S. Sood. (2010). Evaluation of Free Radical Scavenging, Anti-inflammatory and Analgesic potential of Benincasa hippida Seed Extract. International Journal of Pharmacology, 6(5), 652-657. DOI : 10.3923/ijp.2010.652.657
  20. M. Rachchh, P. Yadav, R. Gokani & Jain. (2011). Anti-inflammatory Activity Of Benincasa Hispida Fruit, International Journal of Pharma and Bio Sciences, 2(3), 98-106.
  21. W. H. Kim. (2003). A study on the melanin synthesis inhibition of some natural plant extracts. Masters Dissertation. Duksung Women's University, Seoul. DOI : 10.1080/10937400600882871