Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Identification and Chemotype Profiling of Fusarium Head Blight Disease in Triticale

국내 재배 트리티케일에 발생한 붉은곰팡이병의 다양성 및 독소화학형 분석

  • Yang, Jung-Wook (Crop Cultivation and Environment Research Department, Central Area Crop Breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Joo-Yeon (Crop Cultivation and Environment Research Department, Central Area Crop Breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Mi-Rang (Crop Cultivation and Environment Research Department, Central Area Crop Breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kang, In-Jeong (Crop Cultivation and Environment Research Department, Central Area Crop Breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Jeong, Jung-Hyun (Crop Cultivation and Environment Research Department, Central Area Crop Breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Park, Myoung Ryoul (Central Area Crop Breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Ku, Ja-Hwan (Central Area Crop Breeding Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Wook-Han (Crop Cultivation and Environment Research Department, Central Area Crop Breeding Division, National Institute of Crop Science, Rural Development Administration)
  • 양정욱 (국립식량과학원 중부작물부 재배환경과) ;
  • 김주연 (국립식량과학원 중부작물부 재배환경과) ;
  • 이미랑 (국립식량과학원 중부작물부 재배환경과) ;
  • 강인정 (국립식량과학원 중부작물부 재배환경과) ;
  • 정현정 (국립식량과학원 중부작물부 재배환경과) ;
  • 박명렬 (국립식량과학원 중부작물부 중부작물과) ;
  • 구자환 (국립식량과학원 중부작물부 중부작물과) ;
  • 김욱한 (국립식량과학원 중부작물부 재배환경과)
  • Received : 2021.09.17
  • Accepted : 2021.11.03
  • Published : 2021.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to assess the disease incidence and distribution of toxigenic in Korean triticale. The pathogen of triticale that cause Fusarium head blight were isolated from five different triticale cultivars that cultivated in Suwon Korea at 2021 year. The 72 candidate were classified as a Fusarium asiaticum by morphology analysis and by ITS1, TEF-1α gene sequence analysis. And the results of pathogenicity with 72 isolates on seedling triticale, 71 isolates were showed disease symptom. Also, seven out of 71 Fusarium isolates were inoculated on the wheat, to test the pathogenicity on the different host. The results showed more low pathogenicity on the wheat than triticale. The results of analysis of toxin type with 72 isolates, 64.6% isolates were produced nivalenol type toxin and other 4.6% and 30.8% isolates were produce 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol, respectively. To select fungicide for control, the 72 Fusarium isolates were cultivated on the media that containing four kinds fungicide. The captan, hexaconazole, and difenoconazole·propiconazole treated Fusarium isolates were not showed resistance response against each fungicide. However, six isolates out of 72 isolates, showed resistance response to fludioxonil. This study is first report that F. asiaticum causes Fusarium head blight disease of triticale in Korea.

본 연구에서는 겨울철 사료작물로써 중요성이 증가하고 있는 트리티케일에서 발생한 붉은곰팡이병 원인균의 동정 및 독소 type을 분석하고 트리티케일에 효율적으로 적용 가능한 방제 약제를 선발하고자 분리된 붉은곰팡이 균주의 농약저항성 발생 여부를 분석하였다. 조성, 신조성, 신영, 신성, 세영 등 5개 트리티케일 품종에서 평균 9.15%의 병 발생 이삭률을 보였으며, 병 발생 낱알률은 29.2%로 나타났다. 그 결과 트리티케일에서 발생한 붉은곰팡이병 발생률은 평균 3.52%로 나타났다. 붉은곰팡이병에 감염된 개체로부터 총 91개의 균주가 분리되었으며, 이 중 붉은곰팡이 균주는 총 72개를 분리하였다. 분리한 균주의 형태 및 internal transcribed spacer 1, translation elongation factor 1α 유전자의 염기서열 분석 결과, 트리티케일에서 분리한 균주들은 전부 Fusarium asiaticum으로 동정되었다. 분리 동정된 균주들의 독소 type을 PCR을 통해 분석한 결과, nivalenol (NIV) type 독소는 64.6%, 3-acetyldeoxynivalenol은 4.6%, 15-acetyldeoxynivalenol은 30.8%로 대부분이 NIV type독소를 생성하는 F. asiaticum으로 분석되었다. 최근 봄철 이상 기상현상으로 여러 식량 작물에 붉은곰팡이병 발생률이 높아지고 있다. 트리티케일의 경우 붉은곰팡이병을 방제할 수 있는 등록약제가 없다. 본 실험에서는 보리와 밀에 등록된 약제를 대상으로 트리티케일에서 분리된 붉은곰팡이병 균주들의 약제 내성을 측정한 결과 captan, hexaconazole, difenoconazole·propiconazole 합제는 균사 생장 억제 효과가 있었다. Fludioxonil의 경우 72개 균주 중 6개의 균주가 권장 농도에서 반수영향생육기준치인 42.5 cm를 넘은 47.96±3.15의 균사 생장율을 보여 fludioxonil에 대한 내성이 발생한 것으로 분석되었다. 본 연구는 국내에서 처음 보고 되는 F. asiaticum에 의한 트리티케일의 붉은곰팡이병 발생 사례이며, 또한 붉은곰팡이가 생성하는 독소 타입을 구명하고, 등록 방제 약제가 없는 트리티케일에 효율적으로 방제가 가능한 내성 미발생 약제를 제시하는 중요한 연구 결과이다.

Keywords

Acknowledgement

This study was carried out with the support of Research program for Agricultural Science & Technology Development (Project No. PJ01423102), National Institute of Crop Science, Rural Development Administration, Republic of Korea.

References

  1. Arseniuk, E., Goral, T. and Czembor, H. J. 1993. Reaction of triticale, wheat and rye accessions to graminaceous Fusarium spp. infection at the seedling and adult plant growth stages. Euphytica 70: 175-183. https://doi.org/10.1007/BF00023757
  2. Choi, J.-H., Nah, J.-Y., Jin, H.-S., Lim, S.-B., Paek, J.-S., Lee, M.-J. et al. 2019. Identification and chemotype profiling of Fusarium species in Korean oat. Res. Plant Dis. 25: 157-163. https://doi.org/10.5423/RPD.2019.25.4.157
  3. Ciba, A. J. 1983. Forage production of triticale relative to other spring grains. Agron. J. 75: 610-612. https://doi.org/10.2134/agronj1983.00021962007500040009x
  4. Desjardins, A. E. 2006. Fusarium Mycotoxins: Chemistry, Genetics, and Biology. APS Press, St. Paul, MN, USA. 260 pp.
  5. Ferrigo, D., Raiola, A. and Causin, R. 2016. Fusarium toxins in cereals: occurrence, legislation, factors promoting the appearance and their management. Molecules 21: 627. https://doi.org/10.3390/molecules21050627
  6. Han, O.-K., Park, H.-H., Park, T.-I., Cho, S.-K., Choi, I.-B., Noh, J.-H. et al. 2017. 'Saeyoung', a winter forage triticale cultivare of high-yielding and tolerance to cold. J. Korean Soc. Grassl. Forage Sci. 36: 125-131.
  7. Han, O.-K., Park, H.-H., Park, T.-I., Oh, Y.-J., Ahn, J.-W. and Ku, J.-H. 2019. 'Choyoung', triticale cultivar for forage of early-heading, resistance to lodging and high seed production. J. Korean Soc. Grassl. Forage Sci. 36: 68-74.
  8. Han, O.-K., Park, H.-H., Park, T.-I., Oh, Y.-J., Song, T.-H., Kim, D.-W. et al. 2016. A new early-heading, high-yielding triticale cultivar for forage, 'Shinseong'. J. Korean Soc. Grassl. Forage Sci. 36: 142-149. https://doi.org/10.5333/KGFS.2016.36.2.142
  9. Han, O.-K., Park, T.-I., Park, H.-H., Song, T.-H., Ju, J.-I., Jeung, J.-H. et al. 2012. 'Joseong', a new early-heading forage triticale cultivar for paddy field of double cropping. J. Korean Grassl. Forage Sci. 32: 193-202. https://doi.org/10.5333/KGFS.2012.32.3.193
  10. Heo, H. Y., Park, K. G., Park, H. H., Kim, M. J., Nam, J. H., Lee, C. K. et al. 2002. Cold tolerant, lodging resistant and high grain yielding new forage triticale variety 'Shinyoung'. Treat. Crop Res. 3: 94-98.
  11. Hills, M. J., Hall, L. M., Messenger, D. F., Graf, R. J., Beres, B. L. and Eudes, F. 2007. Evaluation of crossability between triticale (X Triticosecale Wittmack) and common wheat, durum wheat and rye. Environ. Biosafety Res. 6: 249-257. https://doi.org/10.1051/ebr:2007046
  12. Kim, D.-W., Kim, G.-Y., Kim, H.-K., Kim, J., Jeon, S. J., Lee, C. W. et al. 2016. Characterization of nivalenol-producing Fusarium culmorum isolates obtained from the air at a rice paddy field in Korea. Plant Pathol J. 32: 182-189. https://doi.org/10.5423/PPJ.OA.12.2015.0268
  13. Lee, S.-H., Lee, J., Nam, Y.-J., Lee, S., Ryu, J.-G. and Lee, T. 2010. Population structure of Fusarium graminearum from maize and rice in 2009 in Korea. Plant Pathol. J. 226: 321-327.
  14. Lee, T., Lee, S.-H., Lee, S.-H., Shin, J. Y., Yun, J.-C., Lee, Y.-W. et al. 2011. Occurrence of Fusarium mycotoxins in rice and its milling byproducts in Korea. J. Food Prot. 74: 1169-1174. https://doi.org/10.4315/0362-028X.JFP-10-564
  15. Lee, T., Paek, J.-S., Lee, K. A., Lee, S., Choi, J.-H., Ham, H. et al. 2016. Occurrence of toxigenic Fusarium vorosii among small grain cereals in Korea. Plant Pathol J. 32: 407-413. https://doi.org/10.5423/PPJ.OA.05.2016.0123
  16. Leslie, J. F. and Summerell, B. A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing, Ames, IW, USA. 388 pp.
  17. Li, B., Zheng. Z., Liu, X., Cai. Y., Mao. X. and Zhou. M. 2016. Genotypes and characteristics of phenamacril-resistant mutants in Fusarium asiaticum. Plant Dis. 100: 1754-1761. https://doi.org/10.1094/PDIS-02-16-0169-RE
  18. Mergoum, M., Singh, P. K., Pena, R. J., Lozano-del Rio, A. J., Cooper, K. V., Salmon, D. F. et al. 2009. Triticale: a "New" crop with old challenges. In: Cereals. Handbook of Plant Breeding, Vol. 3, ed. by M. J. Carena, pp. 267-287. Springer, New York, NY, USA.
  19. O'Donnell, K., Kistler, H. C., Cigelnik, E. and Ploetz, R. C. 1998. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc. Natl. Acad. Sci. U. S. A. 95: 2044-2049. https://doi.org/10.1073/pnas.95.5.2044
  20. O'Donnell, K., Ward, T. J., Geiser, D. M., Corby Kistler, H. and Aoki, T. 2004. Genealogical concordance between the mating type locus and seven other nuclear genes supports formal recognition of nine phylogenetically distinct species within the Fusarium graminearum clade. Fungal Genet. Biol. 41: 600-623. https://doi.org/10.1016/j.fgb.2004.03.003
  21. Park, J.-M., Shin, S.-H., Kang, C.-S., Kim, K.-H., Cho, K.-M., Choi, J.-S. et al. 2012. Fungicide effects in vitro and in field trials on Fusarium head blight of wheat. Res. Plant Dis. 18: 194-200. https://doi.org/10.5423/RPD.2012.18.3.194
  22. Ryu, J.-G., Lee, S., Lee, S.-H., Son, S.-W., Nam, Y. J., Kim, M. et al. 2011. Natural occurrence of Fusarium head blight and its mycotoxins in 2010-harvested barley and wheat grains in Korea. Res. Plant Dis. 17: 272-279. https://doi.org/10.5423/RPD.2011.17.3.272
  23. Simmonds, N. W. 1976. Evolution of Crop Plants. Longman Group, London, UK. 350 pp.
  24. Yang, L., van der Lee, T., Yang, X., Yu, D. and Waalwijk, C. 2008. Fusarium populations on Chinese barley show a dramatic gradient in mycotoxin profiles. Phytopathology 98: 719-727. https://doi.org/10.1094/PHYTO-98-6-0719
  25. Yin, Y., Liu. X., Li. B. and Ma. Z. 2009. Characterization of sterol demethylation inhibitor-resistant isolates of Fusarium asiaticum and F. graminearum collected from wheat in China. Phytopathology 99: 487-497 https://doi.org/10.1094/PHYTO-99-5-0487
  26. Youn, K. B., Hwang, J. J., Sung, B. R., Lee, J. H., Hur, H. S., Kim, J. G. et al. 1986. A good quality, lodging resistant and high yielding triticale variety 'Sinkihomil' as soiling crop. Res Rept. RDA (Crops) 28: 143-147.
  27. Ward, T. J., Clear, R. M., Rooney, A. P., O'Donnell, K., Gaba, D., Patrick, S. et al. 2008. An adaptive evolutionary shift in Fusarium head blight pathogen populations is driving the rapid spread of more toxigenic Fusarium graminearum in North America. Fungal Genet. Biol. 45: 473-484. https://doi.org/10.1016/j.fgb.2007.10.003
  28. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990 Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a Guide to Methods and Applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, New York, USA.
  29. Zhao, B., He, D. and Wang, L. 2021. Advances in Fusarium drug resistance research. J. Glob. Antimicrob. Resist. 24: 215-219. https://doi.org/10.1016/j.jgar.2020.12.016