The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Effects of Inonotus obliquus Mycelia on the Level of Plasma Glucose and Lipids in Streptozotocin-induced Diabetic Rats

차가버섯 균사체가 Streptozotocin으로 유발된 당뇨 흰쥐의 혈장 포도당과 지질 농도에 미치는 영향

  • Yang, Byung-Keun (Department of Biotechnology, Daegu University) ;
  • Cho, Kai-Yip (School of Science, Food & Horticulture, University of Western Sydney) ;
  • Wilson, Michael A. (School of Science, Food & Horticulture, University of Western Sydney) ;
  • Song, Chi-Hyun (Department of Biotechnology, Daegu University)
  • Published : 2005.12.30
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Abstract

This investigation was undertaken to study the effects of oral administration of Inonotus obliquus mycelia produced by a submerged culture on plasma glucose level and other biochemical parameters in streptozotocin (STZ)-induced diabetic rats. The mycelia, at the dose of 200 mg/kg body weight (BW), substantially reduced the plasma glucose level by as much as 23.1% as compared to the control group. The levels of total cholesterol and triglyceride in plasma were reduced to the extent of 12.6% and 22.6%, respectively. The activities of alanine transaminase (ALT) and aspartate transaminase (AST) were decreased by 27.6% and 21.9%, respectively, under the influence of I. obliquus mycelia. The general components of I. obliquus mycelia were found to contain 5.55% crude ash, 2.35% crude fat, 28.29% crude protein, 9.53% carbohydrate, and 54.28% dietary fiber.

Streptozotocin으로 당뇨가 유발된 흰쥐에 차가버섯 균사체를 2주간 체중 ka당 200 mg으로 경구투여시킨 후 혈장 glucose, total cholesterol, triglyceride, ALT, 및 AST 활성을 관찰하였고, 차가버섯 균사체의 일반성분을 조사하였다. 그 결과 체증증가는 당뇨 대조군에 비해 약 23.5% 증가하였고, 식이섭취량은 21.0% 낮게 나타났으며, 간장 무게는 18.1% 정도 감소하였다. 혈장 glucose 농도는 차가버섯 균사체 투여군이 당뇨 대조군보다 23.1%가 유의하게 감소하였으며, 혈장 total cholesterol과 triglyceride는 각각 12.6%와 22.6%로 낮게 나타났다. 또한 혈장 ALT와 AST 활성은 당뇨 대조군에 비해 차가버섯 균사체 투여군이 27.6%와 21.9% 낮은 활성을 보였다. 상기와 같은 항당뇨 활성을 가진 차가버섯 균사체의 일반성분을 분석한 결과 조회분 5.55%, 조지방 2.35%, 조단백 28.29%, 탄수화물 9.53% 및 식이섬유 54.28%로 구성되어 있었다.

Keywords

References

  1. AOAC. 1995. Official methods of analysis (16th ed). Arlington, VA: Association of Official Chemists
  2. Bailey, C. J. and Day, C. 1989. Traditional plant medicines as treatments for diabetes. Diabetes Care 12: 553-559 https://doi.org/10.2337/diacare.12.8.553
  3. Baynes, J. W. 1991. Role of oxidative stress in development of complications in diabetes. Diabetes 40: 405-412 https://doi.org/10.2337/diabetes.40.4.405
  4. Beppu, H., Maruta, K., Kurner, T. and Kolb, H. 1987. Diabetogenic action of streptozotocin: essential role of membrane permeability. Acta. Endocrinol. (Copdhn) 114: 90-96 https://doi.org/10.1530/acta.0.1140090
  5. Betteridge, J. 1997. Lipid disorders in diabetes mellitus, In: J. C. Pickup and G Williams (eds.), Text Book of Diabetes, Pp. 55.1-55.31. Second ed. Blackwell Science, London, UK
  6. Bursch, W. and Schulte-Hermann, R. 1986. Cytoprotective effect of the prostacyclin derivative hoprost against liver cell death induced by the hepatotoxins carbon terrechloride and bromobenzene. Klin. Wochenschr. 64: 47-50
  7. Cho, Y. J., Kim, H. A., Bang, M. A. and Kim, E. H. 2002. Effects of dietary mushroom on blood glucose levels, lipid concentrations and gllltathlone enzymes in streptozotocininduced diabetic rats. Kor. J. Nutr. 35(2): 183-191
  8. Duncan, D. M. 1957. Multiple-range tests for correlated and heteroscedastic means, Biometrics 13: 164-170 https://doi.org/10.2307/2527799
  9. Frati, A. C., Gordillo, B. E., Altamirano, P., Ariza, C. R., Cortes-Franco, R., Chavez-Negrete, A. and Islas-Andrade, S. 1991., Influence of nopal intake upon fasting glycemia in type II diabetic and healthy subjects. Archivos de Investigation Medica. 22: 51-56
  10. Furuse, M., Kimura, C., Mabayo, R. T., Takahashi, H. and Okumura, J. 1993. Dietary sorbose prevents and improves hyperglycemia in genetically diabetic mice. J. Nutr. 123: 59-65 https://doi.org/10.1093/jn/123.1.59
  11. Gray, A. M. and Flatt, P. R. 1998. Insulin-releasing and insulinlike activity of Agaricus campestris (mushroom). J. Endocrinol. 157: 259-266 https://doi.org/10.1677/joe.0.1570259
  12. Grey, N. J., Karls, L. and Kipnis, D. M. 1975. Physiologic mechanism in the development of starvation ketosis in man. Diabetes 24: 10-16 https://doi.org/10.2337/diabetes.24.1.10
  13. Ham, S. S., Oh, S. W., Kim, Y. K, Shin, K. S., Chang, H. Y. and Chung, G. H. 2003. Antimutagenic and cytotoxic effects of ethanol extract from the Inonotus obliquus. J. Korean Soc. Food Sci. Nutr. 32: 1088-1094 https://doi.org/10.3746/jkfn.2003.32.7.1088
  14. Iyorra, M. D. and Paya, H, 1988. Hypoglycemic and insulin release effects of torrnentic acid: a new hypoglycemic natural product. Planta. Medica. 55: 282-286 https://doi.org/10.1055/s-2006-962433
  15. Johnson, I. T. 1991. The biological effects of dietary fiber in small intestine. In deitary Fiber: Chemical and Biological Aspects, Pp. 151-163. South A. T., Waldron K., Johnson I. T., and Fenwick G. R. ed. The Royal Society of Chemistry, London, UK
  16. Junod, A., Lambert, A. E., Orci, L., Pictet, R., Gonet, A. E. and Renold, A. E. 1967. Studies of the diabetogenic action of streptozotocin, Proc. Soc. Exp. Biol.Med. 126: 201-205
  17. Kahlos, K. and Hiltunen, R. 1983. Identification of some lanostane type triterpenes from Inonotus obliquus. Acta. Pharm. Fenn. 92: 200-224
  18. Kahlos, K., Kangas, L. and Hitunen, R. 1987. Antitumor activity of some compounds and fraction an n-hexane extract of Inonotus obliquus. Acta. Pharm. Fenn. 96: 33-40
  19. Kang, T. S., Kang, M. S., Sung, J. M., Kang, A. S., Shon, H. R. and Lee, S. Y. 2001. Effect of Pleurotus eryngii on the blood glucose and cholesterol in diabetic rats. Kor. J. Mycol. 29: 86-90
  20. Kim, D. H., Yang, B. K., Hur, N. J., Das, S., Yun, J. W., Choi, Y. S. and Song, C. H. 2001a. Hypoglycemic effects of mycelia produced from a submerged culture of Phellinus linteus (Berk, et curt) Teng (Aphyllophoromycetideae) in streptozotocininduced diabetic rats. Int. J. Med. Mushr. 3: 21-26
  21. Kim, D. H., Yang, B. K., Jeong, S. C., Hur, N. J., Das, S., Yun, J. W., Choi, J. W., Lee, Y. S. and Song, C. H. 2001b. A preliminary study on the hypoglycemic effect of the exo-polyrners produced by five different medicinal mushrooms, J. Microbiol. Biotechnol. 11: 167-171
  22. Lazarus, S. S. and Shapiro, S. H. 1972. Streptozotocin-induced diabetes and islet cell alteration in rabbits. Diabetes 21: 129-237 https://doi.org/10.2337/diab.21.3.129
  23. Lee, M. Y., Kim, M. K., Shin, J. G. and Kim, S. D. 2004. Dietary effect of hemicellulose from soy fiber on blood glucose and cholesterol content in streptozotocin-induced diabetic rats. J. Korean Soc. Food. Sci. Nutr. 33: 1119-1125 https://doi.org/10.3746/jkfn.2004.33.7.1119
  24. Lee, S. O., Kim, M. J., Kim, D. G. and Choi, H. J. 2005. Antioxidative activities of temperature-stepwise water extracts from Inonotus obliquus. J. Korean Soc. Food Sci. Nutr. 34: 139-147 https://doi.org/10.3746/jkfn.2005.34.2.139
  25. Mizuno, T., Zhuang, C., Abe, K., Okamoto, H., Kino, T., Ukai, S., Leclerc, S. and Meijer, L. 1999. Antitumor and hypoglycemic activities of polysaccharides from the Sclerotia and Mycelia ofInonotus obliquus (Pers.: Fr.) Pil. (Aphyllophoromycetideae). Int. J. Med. Mushr. 1; 301-316 https://doi.org/10.1615/IntJMedMushr.v1.i4.20
  26. Nelson, R. W., Ihle, S. L., Lewis, L. D., Salisbury, S. K., Miller, T., Bergdall, V. and Bottoms, G. D. 1991. Effects of dietary fiber supplementation on glycemic control in dogs with alloxan-induced diabetes mellitus. Am. J. Vet. Res. 52: 2060-2066
  27. Niall, M. G., Rosaleen, A, M., Daphne, O., Patrick, B. C., Alan, H. J. and Gerald, H. 1990. Cholesterol metabolism in alloxaninduced diabetic rabbits. Diabetes 39: 626-636 https://doi.org/10.2337/diabetes.39.5.626
  28. Prosky, L., Asp, N. G., Schweizer, T. F., Devries. J. W. and Furda, I. 1992. Determination of insoluble, soluble and total dietary fiber in foods and food products: Collaborative study. J. Assoc. Off. Chem. 75: 360-367
  29. Reaven, G. M. 1988. Role of insulin resistance in human disease. Diabetes 37: 1595-1607 https://doi.org/10.2337/diabetes.37.12.1595
  30. Rho, H. M., Choi, M. A. and Koh, J. B. 1998. Effects ofraw soy flour (yellow ,and black) on serum protein concentrations and enzyme activity in streptozotoein-diabetic rats. J. Korean Soc. Food Sci. Nutr. 27: 724-730
  31. Saudek, C. D. and Eder, H. A. 1979. Lipid metabolism in diabetes mellitus. Am. J. Med. 66: 843-849 https://doi.org/10.1016/0002-9343(79)91126-4
  32. Shivrina, A. N. 1967. Chemical characteristics of compounds extracted from Inonotus obliquus. Chem. Abstr. 66: 17271-17279
  33. Wahren, J., Felig, P., Cerasi, E. and Luft, R. 1972. Splanchnic and peripheral glucose amino acid metabolism in diabetes mellitus. J. Clin. Invest. 51: 870-876
  34. Woo, J. Y., Baek, K. Y. and Han, J. P. 1998. Effect of royal jelly on therapy and prevention of streptozotocin-induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 27: 1267-1272
  35. Yang, B. K., Kim, D. H. and Song, C. H. 2002. Production of Lentinus edodes mycelia in submerged culture and it's hypoglycemic effect in diabetic rats. Kor. J. Mycol. 30: 131-135 https://doi.org/10.4489/KJM.2002.30.2.131