Composition and Cytotoxicity of Essential Oil from Korean rhododendron (Rhododendon mucronulatum Turcz. var. ciliatum Nakai)

털진달래(Rhododendon mucronulatum Turcz. var. ciliatum Nakai) 정유의 성분분석과 독성평가

  • Park, Yu-Hwa (Department of Biological Environment, Kangwon National University) ;
  • Kim, Song-Mun (Department of Biological Environment, Kangwon National University)
  • 박유화 (강원대학교 자원생물환경학과) ;
  • 김성문 (강원대학교 자원생물환경학과)
  • Published : 2008.09.30

Abstract

The essential oil was obtained from the aerial part of Rhododendon mucronulatum Turcz. var. ciliatum Nakai by steam distillation, samples were collected by headspace (HS) and solid-phase microextraction (SPME) methods, and the compositions of the oil were analyzed by gas chromatography-mass spectrometry (GC-MS). Nineteen constituents were identified from the essential oil: 15 carbohydrates, 3 alcohols, and 1 acetates. Major constituents were 2-${\beta}$-pinene (16.1%), camphene (11.9%), ${\delta}$-3-carene (11.4%), d,l-limonene (9.5%), and ${\gamma}$-terpinene (9.5%). By SPME extraction, seventeen constituents were identified: 13 hydrocarbons, 1 alcohol, 1 nitrogen-containing compound, 1 acetate, and 1 amine. Major constituents of the SPME-extracted sample were cam phene (19.6%), 2-${\beta}$-pinene (18.0%), ${\delta}$-3-carene (17.4%), trimethyl hydrazine (9.7%), ${\gamma}$-terpinene (8.5%), and d,l-limonene (5.5%). By HS extraction, thirteen constituents were identified: 11 hydrocarbons, 1 alcohol, and 1 nitrogen-containing compound. Major constituents of the HS-extracted sample were camphene (25.8%), ${\delta}$-3-carene (24.8%), 2-${\beta}$-pinene (20.2%), d,l-limonene (5.4%), tricyclene (5.1%) and trimethyl hydrazine (4.6%). The fragrance of the essential oil was coniferous, balsamic, and woody, and the $IC_{50}$ value of the essential oil was 0.030 ${\mu}g/mg$ in MTT assay using UaCaT keratinocyte cell line.

우리나라의 자생식물인 털진달래(Rhododendon mucronulatum Turcz. var. ciliatum Nakai)지상부위로부터 정유를 얻고, 휘발성분을 headspace(HS)와 solid-phase microextraction(SPME)법으로 흡착시킨 다음 GC-MS로 분석하였다. 털진달래 정유에는 탄화수소 15종, 알코올 3종, 아세테이트 1종 등 총 19종의 화학성분이 함유되어 있었으며, 다함량 성분은 2-${\beta}$-pinene(16.1%), camphene(11.9%), ${\delta}$-3-carene(11.4%), d,l-limonene(9.5%), and ${\gamma}$-terpinene(9.5%)이었다. SPME법으로 흡착된 시료에는 탄화수소 13종, 알코올 1종, 질소화합물 1종 등 총 17종의 휘발성 화학물질이 함유되어 있었고, 이들 중 다함량 성분은 camphene(19.6%), 2-${\beta}$-pinene(18.0%), ${\delta}$-3-carene(17.4%), timethyl hydrazine(9.7%), ${\gamma}$-terpinene(8.5%), and d,l-limonene(5.5%)이었다. 그리고 HS법으로 흡착된 시료에는 탄화수소 11종, 알코올 1종, 질소화합물 1종 등 총 13종의 휘발성 화학물질이 함유되어 있었으며, 이들 중 다함량 성분은 camphene(25.8%), ${\delta}$-3-carene(24.8%), 2-${\beta}$-pinene(20.2%), d,l-limonene(5.4%), tricyclene(5.1%), trimethyl hydrazine(4.6%)이었다. 털진달래 정유의 향취는 coniferous, balsamic, woody하였으며, HaCaT 각질형성세포에 대한 $IC_{50}$ 값은 0.030 ${\mu}g/mg$이었다.

Keywords

References

  1. Koh, J. G., Moon, M. O. and Koh, S. C. (1999) The vegetation and plant resources of Paeknokdam, the crater of Mt. Halla. Kor. J. Plant Res. 12, 221-233
  2. Kim, G. T. and Baek, G. J. (1998) Studies on the structure of forest community at Taechongbong-Hangyeryong area in Soraksan National Park. Kor. J. Env. Eco. 11, 397-406
  3. Lee, T. B. (2003) Coloured flora of Korea. vol. 2. Hyangmunsa, pp. 112
  4. Jung, B. S. and Shin, M. G. (1990) Hyang Yak (Herbal) Dictionary. Younglim-sa, pp.1001
  5. An, B. J., Lee, C. E., Son, J. H., Lee, J. Y., Choi, G. H. and Park, T. S. (2005) Antioxidant, anticancer and tyrosine inhibition activities of extracts from Rhododendron mucronulatum T. J. Korean Soc. Appl. Biol. Chem. 48, 280-284
  6. Lee, B. B., Chun, J. H., Lee, S. H., Park, H. R., Kim, J. M., Park, E. and Lee, S. C. (2007) Antioxidative and antigenotoxic activity of extracts from Rhododendron mucronulatum Turcz. flowers. J. Korean Soc. Food Sci. Nutr. 36, 1628-1632 https://doi.org/10.3746/jkfn.2007.36.12.1628
  7. Cho, Y. J., Ju, I. S., Chun, S. S., An, B. J., Kim, J. H., Kim, M. U. and Kwon, O. J. (2008) Screening of biological activities of extracts from Rhododendron mucronulatum Turcz. flowers. J. Korean Soc. Food Sci. Nutr. 37, 276-281 https://doi.org/10.3746/jkfn.2008.37.3.276
  8. An, B. J., Lee, J. T., Lee, C. E., Son, J. H., Lee, J. Y. and Park, T. S. (2005) A study on the development of cosmeceutical ingredient, Rhododendron mucronulatum, and the application of rheology properties. J. Korean Soc. Appl. Biol. Chem. 48, 273-279
  9. Park, Y. H. and Kim, S. (2008) Composition and cytotoxicity of essential oil extracted by steam distillation from Caryopteris incana Miq. in Korea. J. Korean Soc. Appl. Biol. Chem. 51, submitted
  10. Choi, H. J., Wang, H. Y., Kim, Y. N., Heo, S. J., Kim, N. K., Jeong, M. S., Park, Y. H. and Kim, S. (2008) Composition and cytotoxicity of essential oil extracted by steam distillation from horseweed (Erigeron canadensis L.) in Korea. J. Korean Soc. Appl. Biol. Chem. 51, 55-59
  11. Park, Y. H., Choi, H. J., Wang, H. Y., Kim, H. Y., Heo, S. J., Kim, K. H., Kim, Y. N. and Kim, S. (2007) Volatile components of Erigeron canadensis L. in Korea. The Korean Soc. of Weed Sci. 27, 268-274
  12. Lee, Y. S. (2006) Development of aroma oil from Gangwon native plants. MSc Thesis, Kangwon National University
  13. Indou, M. (1996) In Synthetic perfumes. pp. 1001, Chemical Industry Ilbo