Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Development of Green Tea Beverage with Organic Tea Leaves

유기농 녹차잎을 이용한 녹차음료의 개발

  • An, Mi-Kyoung (Department of Food Science and Technology, Dongguk University) ;
  • Ahn, Jun-Bae (Department of Food Service Industry, Seowon University) ;
  • Lee, Kwang-Geun (Department of Food Science and Technology, Dongguk University)
  • Published : 2008.10.31
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Abstract

In this study, organic tea leaves were characterized with the aim of developing an organic beverage process. The green tea leaves grown using organic farming techniques were collected in Haenam, Korea. Catechins in green tea leaves were extracted by chloroform and ethyl acetate and these were then analyzed quantitatively and qualitatively by HPLC (high pressure liquid chromatography). The color and pH values of the green tea extracts were also measured. The catechin levels of April-harvested, May-harvested and June-harvested, semi-fermented leaves at 0.5% were 66.24, 29.19, 57.11, and 5.27 ${\mu}g/mL$, respectively. Among the detected catechins, the level of (-)-epigallocatechin gallate was the highest while that of (-)-epigallocatechin was not detected. The June-harvested leaves were selected as raw material for development of the green tea beverage, based on the levels of catechins, economic viability and yield of tea extract. As the level of extract increased, the levels of catechins of 0.1, 0.2, 0.5% also increased by 1.5, 11.78 and 41.01 times. From the results of the sensory evaluation of June-harvested leaf-extract, the sensory score of color was the highest in 0.1%, while the flavor and overall quality were the highest in 0.2%.

본 연구는 유기농 녹차 음료를 개발하기 위해 유기농 다엽의 카테킨류 함량을 비롯한 물리화학적 특성을 분석하였다. 다엽은 일체의 화학비료와 농약을 사용하지 않고 유기농 기법으로 재배한 시료를 사용하였고 채취시기가 다른 4월, 5월, 6월차와 반발효차를 수매하여 실험에 사용하였다. HPLC를 이용하여 다엽의 카테킨류 함량을 정량 정성 분석하였으며 녹차추출물의 색도와 pH를 측정하였다. 0.5%의 4월, 5월, 6월, 반발효차의 카테킨류는 각각 66.24, 29.19, 57.11, 5.27 ${\mu}g/mL$을 함유하고 있었으며, 카테킨류 중에서 EGCg의 함량이 가장 높았으나 EGC는 검출되지 않았다. 유기농 녹차 음료의 생산을 위해 카테킨류의 함량, 다엽의 가격, 수확량을 고려하여 6월차를 선택하였다. 6월차를 0.05, 0.1, 0.2, 0.5%로 추출하여 카테킨류를 정량한 결과, 0.1, 0.2, 0.5%는 0.05%에 비해 각각 1.50, 11.78, 41.09배 증가하였다. 6월차는 농도가 증가할수록 밝기가 증가하였고, 가장 밝고 녹색과 황색의 색이 짙었다. 반발효차의 pH는 5.83이었으며 나머지는 6.33-6.38의 범위를 가졌다. 6월차의 기호도 및 관능평가를 실시한 결과, 색상 기호도는 0.1%, 향기호도와 전체적인 기호도는 0.2% 추출물이 가장 우수한 평가를 받았고 단맛, 신맛, 떫은 맛의 정도를 평가한 결과 0.2%가 음용하기에 가장 적절하리라 사료된다. 또한 음료 제작 후, 저장 기간에 따른 녹차의 색 변화를 줄이기 위해 저장성 실험 결과 탄산수소나트륨을 0.03%를 첨가할 필요가 있다.

Keywords

References

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