Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Enhancement of Skin Immune Activation Effect of Collagen Peptides Isolated from Asterias amurensis

불가사리 유래 콜라겐 펩타이드의 피부 면역 증진 효과

  • Jeong, Hyang-Suk (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Kwon, Min-Chul (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Han, Jae-Gun (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Ha, Ji-Hye (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Jin, Ling (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Jin-Chul (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Kwak, Hyeong-Geun (2SKY007 Co.) ;
  • Hwang, Bo-Young (2SKY007 Co.) ;
  • Lee, Hyeon-Yong (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University)
  • 정향숙 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 권민철 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 한재건 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 하지혜 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 김령 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 김진철 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 곽형근 ((주)SKY007) ;
  • 황보영 ((주)SKY007) ;
  • 이현용 (강원대학교 BT특성화학부대학 생물소재공학과)
  • Published : 2008.10.31
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Abstract

Low molecular peptides were isolated from Asterias amurensis via SDS-PAGE. The peptides were separated via consecutive gel filtration as five fractions (F1-F5) according to molecular weights, based on the results of MALDI-TOF MS analysis. The molecular weight of the most active peptide was estimated as 15,000 daltons. The peptide showed cytotoxicity on normal human fibroblast cells at levels as low as 20% when 1.0 mg/mL of the samples was added. The peptide also exhibited higher levels of nitric oxide production from macrophages than the lipopolysaccaharides. It was determined that prostaglendin $E_2$ production was significantly inhibited, up to 127.8% as compared to the control. The low molecular peptide inhibited hyaluronidase activity as 535.7 ${\mu}g/mL$ of $IC_{50}$. It can be concluded that the relatively low molecular weight peptide, fucoidan, from A. amurensis has excellent cosmetic and immunomodulatory activities, which can be considered as a possible resource of new cosmetic agents for skin immunomodulation.

불가사리 콜라겐으로부터 저분자 활성 펩타이드를 SDS-PAGE로 분리하고 MALDI-TOF MS를 이용한 분자량 측정을 통해fraction별로 F1-F5의 5개 시료로 나누어 실험을 수행하였다. 인간 fibroblast를 이용한 세포독성 측정에서 1.0 mg/mL 농도의 시료 첨가를 통한 모든 조건에서 20%의 이하의 세포독성을 나타냄에 따라 불가사리 유래 펩타이드가 세포 수준에서 유의할만한 세포독성을 나타내지 않음 확인하였다. 대식세포를 이용한 $NO^-$ 생성능 측정에서는 시료만 처리했을 경우 무처리 대조군과 비교하여 큰 변화를 관찰 할 수 없었으나, LPS와의 혼합 처리를 통해 F2가 최대 33.8 ${\mu}M$의 NO의 생성량을 나타냄에 따라 시료첨가를 통해 면역 기작이 획기적으로 증진되는 효과를 보였다. 인간 fibroblast인 CCD-986sk의 생육에서 UV 조사를 통한 $PGE_2$ 발현도 측정 실험에서는 시료첨가를 통해 $PGE_2$의 발현이 농도 의존적으로 감소되는 결과를 나타내었으며, 세포 증식에 따른 콜라겐 합성능의 비교 결과에서는 시료 첨가를 통해 모든 조건의 콜라겐 생성량이 농도 의존적으로 증가하는 경향을 나타내었다. 특히 F1과 F2가 1.0 mg/mL의 농도에서 각각 121.2$\pm$3.7%과 127.8$\pm$1.1%를 나타내며 양성 대조군에 비해 높은 콜라겐 합성량을 나타내었다. 면역 활성 탐색을 위한 hyaluronidase 저해효과 측정에서도 F2가 535.7 ${\mu}g/mL$로 가장 낮은 $IC_{50}$을 나타내었다. 이상의 실험을 통해 불가사리 유래 콜라겐의 저분자 펩타이드는 세포독성 낮고 NO 생성 촉진 활성, $PGE_2$ 발현 저해 및 콜라겐의 생성 촉진 효과를 가지는 것을 확인할 수 있었다. 이러한 결과는 불가사리 유래 콜라겐 펩타이드의 기능성 향장 소재로서 활용 가능성을 확인해주는 긍정적인 결과로 향후 추가적인 연구를 통해 불가사리의 향장소재로서의 활용이 기대된다.

Keywords

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