Analysis of Nutritional Components and Evaluation of Functional Activities of Sasa borealis Leaf Tea

조릿대 잎차의 영양성분 분석 및 기능성 평가

  • Jeong, Chang-Ho (Division of Applied Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Choi, Sung-Gil (Division of Applied Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Heo, Ho-Jin (Division of Applied Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University)
  • 정창호 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 최성길 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 허호진 (경상대학교 응용생명과학부, 농업생명과학연구원)
  • Published : 2008.10.31

Abstract

In this study, the nutritional components and functional activities of Sasa borealis leaf tea were evaluated. The proximate compositions were as follows; moisture 5.68%, crude protein 16.38%, crude fat 4.68%, nitrogen free extracts 32.37%, crude fiber 32.36%, and ash 8.53%, respectively. The mineral elements were as follows: K 2,133.83, Ca 1,144.09 and P 543.00 mg%, respectively. The amino acid contents of the Sasa borealis leaf tea were very rich in proline (1,275.26 mg/100 g) and deficient in cystine (71.49 mg/100 g). The major fatty acid components were linoleic acid (50.52%), palmitic acid (18.52%), and oleic acid (14.16%). Finally, based on our sensory evaluations, the $80^{\circ}C$ extracted Sasa borealis leaf tea evidenced the best overall quality. The contents of total phenol and total flavonoids of the 80% methanol and hot water extracts were 15.09, 7.69 mg/g and 12.03, 6.12 mg/g, respectively. The DPPH and $ABTS^+$ radical scavenging activities of the 80% methanol extract from Sasa borealis leaf tea were 86.87% and 83.85% at a concentration of 1.25 mg/mL. The 80% methanol and hot water extracts evidenced reducing power and inhibitory effects against acetylcholinesterase in a dose-dependent manner.

조릿대 잎을 이용한 가공품 개발에 대한 기초자료로 활용하기 위하여 잎차의 영양성분 및 기능성을 평가하였다. 일반성분은 수분 5.68%, 조단백 16.38%, 조지방 4.68%, 가용성 무질소물 32.37%, 조섬유 32.36% 및 회분 8.53%로 각각 나타났다. 주요 무기성분으로는 K 2,133.83, Ca 1,144.09 및 P 543.00 mg/100 g이었으며, 아미노산중 proline이 1,275.26 mg/100 g으로 가장 높은 함량을 보였고, cystine이 71.49 mg/100 g으로 가장 낮은 함량을 보였다. 주요 지방산으로는 linoleic acid(50.52%), palmitic acid(18.52%) 및 oleic acid(14.16%)였으며, 관능검사 결과 $80^{\circ}C$에서 추출한 조릿대 잎차가 가장 높은 기호도를 보였다. 80% 메탄올 및 열수추출물의 총 페놀과 플라보노이드 함량을 측정한 결과 15.09, 7.69 mg/g과 12.03$\pm$0.67, 6.12$\pm$0.29 mg/g으로 각각 나타났다. DPPH와 $ABTS^+$ 라디칼 소거활성을 측정한 결과 80% 메탄올추출물 농도 1.25 mg/mL에서 각각 86.87%와 83.85%의 소거활성을 보였으며, 환원력 및 acetylcholinesterase(퇴행성뇌신경질환의 일종인 치매 유발 효소) 저해활성은 농도 의존적인 경향을 나타내었다.

Keywords

References

  1. Fang YZ, Yang S, Wu G. Free radical, antioxidant, and nutrition. Nutrition 18: 872-879 (2002) https://doi.org/10.1016/S0899-9007(02)00916-4
  2. Morrissey PA, O'Brien NM. Dietary antioxidant in health and disease. Int. Dairy J. 8: 463-472 (1998) https://doi.org/10.1016/S0958-6946(98)00070-3
  3. Willet WC. Diet and health: what should we eat. Science 254: 532-537 (1994)
  4. Slavin JL, Jacobs D, Marquart L. Whole-grain consumption and chronic disease: Protection mechanism. Nutr. Cancer 27: 14-21 (1997) https://doi.org/10.1080/01635589709514495
  5. Temple NJ. Antioxidants and disease: More question than answers. Nutr. Res. 20: 449-459 (2000) https://doi.org/10.1016/S0271-5317(00)00138-X
  6. Feskanich D, Ziegler RG, Michaud DS, Giovannucci EL, Speizer FE, Willett WC, Colditz GA. Prospective study of fruit and vegetable consumption and risk of lung cancer among men and women. J. Natl. Cancer I. 92: 1812-1823 (2000) https://doi.org/10.1093/jnci/92.22.1812
  7. Ames BN, Shigenaga MK, Hagwn TM. Oxidants, antioxidant, and the degenerative disease of aging. P. Natl. Acad. Sci. USA 90: 7915-7922 (1993)
  8. Chung DK, Yu R. Antimicrobial activity of bamboo leaves extract on microorganisms related to kimchi fermentation. Korean J. Food Sci. Technol. 27: 1035-1038 (1995)
  9. Kim MJ, Byun MW, Jang MS. Physiological and antibacterial activity of bamboo (Sasa coreana Nakai) leaves. J. Korean Food Sci. Nutr. 25: 135-142(1996)
  10. Shin JH, Choi DJ, Sung NJ. Nutritional properties of yakju brewed with natural plants. Korean J. Food Nutr. 17: 18-24 (2004)
  11. Shin MK, Han SH. Effects of methanol extracts from bamboo (Pseudosasa japonica Makino) leaves extracts on lipid metabolism in rats fed high fat and high cholesterol diet. Korean J. Diet. Culture 17: 30-36 (2002)
  12. Kim EY, J EY, Lim HS, Heo YR. The effects of the Sasa borealis leaves extract on plasma adiponectin, resistin, C-reactive protein and homocysteine levels in high fat diet-induced obses C57/ BL6J mice. Korean J. Nutr. 40: 303-311 (2007)
  13. Lee MJ, Moon GS. Antioxidative effects of Korean bamboo trees, 'Wang-dae', 'Som-dae', 'Maengjong-Juk', 'Jolit-dae' and 'O-juk'. Korean J. Food Sci. Technol. 35: 1226-1232 (2003)
  14. Ju IO, Jung GT, Ryu J, Choi JS, Choi YG. Chemical components and physiological activities of bamboo (Phyllostachys bambusoides Starf) extracts prepared with different methods. Korean J. Food Sci. Technol. 37: 542-548 (2005)
  15. Ko BS, Jun DW, Jang JS, Kim JH, Park SM. Effect of Sasa Borealis and white lotue roots and leaves on insuline action and secretion in vitro. Korean J. Food Sci. Technol. 38 : 114-120 (2006)
  16. Lee J, Jeong YH, Jang DS, Seo EK. Three terpenes and one phenolic compound from Sasa borealis. J Appl. Biol. Chem. 50: 13- 16 (2007)
  17. Park HS, Lim JH, KIm HJ, Choi HJ, Lee IS. Antioxidant flavone glycosides from the leaves of Sasa borealis. Arch. Pharm. Res. 30: 161-166 (2007) https://doi.org/10.1007/BF02977689
  18. Chung MJ, Lee SJ, Shin JH, Jo JS, Sung NJ. The components of the sap from birches, bamboos and darae. J. Korean Soc. Food Nutr. 24: 727-733 (1995)
  19. Lee JY, Ju JC, Park HJ, Heu ES, Choi SY, Shin JH. Quality characteristics of cookies with bamboo leaves powder. Korean J. Food Nutr. 19: 1-7 (2006)
  20. AOAC. Official Methods of Analysis of AOAC Intl. 13th ed. 920.39, 934.01, 942.05, 954.01 and 974.06, Association of Official Analytical Chemists, Washington DC, USA (1990)
  21. Jeong CH, Shim KH. Quality characteristics of sponge cake with addition of Pleurotus eryngii mushroom powders. J. Korean Soc. Food Sci. Nutr. 33: 716-722 (2004) https://doi.org/10.3746/jkfn.2004.33.4.716
  22. Jeong CH, Shim KH. Chemical composition and antioxidative activities of Platycodon grandiflorum leaves and stems. J. Korean Soc. Food Sci. Nutr. 35: 511-515 (2006) https://doi.org/10.3746/jkfn.2006.35.5.511
  23. Metcalf LD, Schmits AA, Pelka JR. Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Anal. Chem. 38: 514-515 (1966) https://doi.org/10.1021/ac60235a044
  24. Graham HD. Modified prussian blue assay for total phenolic compound. J. Agr. Food Chem. 40: 801-805 (1992) https://doi.org/10.1021/jf00017a018
  25. Jeong CH, Bae YI, Lee HJ, Shim KH. Chemical components of propolis and its ethanolic extracts. J. Korean Soc. Food Sci. Nutr. 32: 501-505 (2003) https://doi.org/10.3746/jkfn.2003.32.4.501
  26. Blois MA. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-200 (1958) https://doi.org/10.1038/1811199a0
  27. Pellegrin N, Re R, Yang M, Rice-Evans C. Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Method Enzymol. 299:379-389 (1998)
  28. Yen GH, Chen HY. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agr. Food Chem. 45: 27-32 (1995)
  29. Ellman GL, Doutney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination acetylcholinesterase activity. Biochem. Phamacol. 7 : 88-95 (1961) https://doi.org/10.1016/0006-2952(61)90145-9
  30. Lee KH, Park HC, Her ES. Statistics and Date Analysis Method. Hyoil Press, Seoul, Korea. pp. 253-296 (1998)
  31. Ko MS. Chemical components in stalks and leaves of Sasa borealis Makino and antioxidant and antimicrobial activities of extracts. Korean J. Food Preserv. 15: 125-132 (2008)
  32. Kim NK, Cho SH, Lee SD, Ryu JS, Shim KH. Chemical properties of hot water extracts from bamboos (Phyllostachys sp.). Korean J. Postharv. Sci. Technol. 8: 469-474 (2001)
  33. Han SJ, Koo SJ. Study on the chemical composition in bamboo shoot, lotus root and burdock. Free sugar, fatty acid, amino acid, and dietary fiber contents. Korean J. Soc. Food Sci. 9: 82-87 (1993)