The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effect of Wongisaengmaek-san Extract on Antioxidative and Anti-Fatigue Activity

원기생맥산(元氣生脈散)의 추출물별 항산화작용과 쥐의 운동피로 회복효과

  • Kim, Yoo-Seung (3rd Department of Internal Medicine, College of Oriental Medicine, Kyung-hee University) ;
  • Ryu, Bong-Ha (3rd Department of Internal Medicine, College of Oriental Medicine, Kyung-hee University) ;
  • Kim, Jin-Sung (3rd Department of Internal Medicine, College of Oriental Medicine, Kyung-hee University)
  • 김유승 (경희대학교 한의과대학 비계내과학 교실) ;
  • 류봉하 (경희대학교 한의과대학 비계내과학 교실) ;
  • 김진성 (경희대학교 한의과대학 비계내과학 교실)
  • Published : 2009.03.31
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Abstract

Objectives : This study aimed to evaluate the effect of Wongisaengmaek-san (extracted with water or 50% ethanol) on antioxidative activity and recovery of fatigue induced by weight-loaded forced swimming exercise. Methods : Antioxidant activity of Wongisaengmaek-san was evaluated in terms of total amount of polyphenol, 1.1-diphenyl-2-picrylhvdroxyl (DPPH) radical scavenging activity and xanthine oxidase inhibition. The anti-fatigue effect of Wongisaengmaek-san was investigated using an acute weight-loaded forced swimming test by monitoring swimming test times and blood biochemical parameters creatinine. BUN, lactic acid dehydrogenase (LDH), free fatty acid (FFA) and lactic acid (LA). Results : 1. 50% EE (ethanol extract) had 2.3 times higher amount of total polyphenol compared to water extract. 2. It was identified that 50% ethanol extract showed enhancement of xanthine oxidase inhibition and DPPH scavenging effect in vitro. 3. According to anti-fatigue effect in the scale of 5% weight loaded mouse swimming test, both water and 50% ethanol extract showed significant improvement of swimming time elongation respectively, and 50% ethanol extract induced a more positive result. 4. After swimming for 1% weight-loaded mouse. water extract and 50% ethanol showed significant anti-fatigue effect with manifestation of blood sampling among both of the intervention: 50% ethanol extract showed a greater result. Conclusions : 50% ethanol extract of Wongisaengmaek-san has more effective antioxidant activity and anti-fatigue than water extract.

Keywords

References

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