DOI QR코드

DOI QR Code

Biological Activities in the Extract of Fermented Codonopsis lanceolata

발효더덕 추출물의 생리활성

  • Park, Sung-Jin (Dept. of Tourism Food Service Cuisine, Hallym College) ;
  • Song, Seong-Won (Yooyoung Pharmaceutical Co.) ;
  • Seong, Dong-Ho (Dept. of Biotechnology, Yonsei University) ;
  • Park, Dong-Sik (Funtional Food & Nutrition Division, Rural Development Administration) ;
  • Kim, Seung-Seop (Dept. of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Gou, Jinyu (Dept. of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Ahn, Ju-Hee (Dept. of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Yoon, Won-Byung (Dept. of Food Science and Biotechnology, School of Biotechnology, Kangwon National University) ;
  • Lee, Hyeon-Yong (Dept. of Biomaterials Engineering, College of Bioscience and Biotechnology)
  • 박성진 (한림성심대학 관광외식조리과) ;
  • 송성원 ((주)유영제약) ;
  • 성동호 (연세대학교 생명시스템대학 생명공학과) ;
  • 박동식 (농촌진흥청 국립농업과학원 한식세계화연구단 기능성식품과) ;
  • 김승섭 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 고징유 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 안주희 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 윤원병 (강원대학교 BT특성화학부대학 식품생명공학과) ;
  • 이현용 (강원대학교 BT특성화학부대학 생물소재공학과)
  • Published : 2009.08.31

Abstract

To investigate the applicability of hot water extract from Codonoposis lanceolata (CL) and fermented Codonoposis lanceolata (FCL) as functional food and cosmeceutical material, its total phenolic contents, total flavonoids, electron donating ability (EDA), SOD-like activity, nitrate-scavenging ability (NSA), reducing power were examined. Total phenolic contents of CL and FCL were 0.54 mg/100 g and 2.79 mg/100 g, respectively, and total flavonoids contents were estimated as 2.26 mg/100 g for CL and 6.16 mg/100 g FCL. The EDA of CL and FCL were 8.0$\sim$17.9% and 32.9$\sim$74.9%. The SOD-like activities of CL and FCL were 0.6$\sim$16.5% and 3.5$\sim$21.6%, respectively, and the activity was dependent on the sample concentration. The NSA was pH dependent, and was the highest at pH 1.2 and the lowest at pH 6.0. The NSA of FCL was higher than that of CL. The FCL extract showed the highest reducing power (0.65) at the concentration of 1,000 $\mu g$/mL. Based on the above results, we deemed that the FCL might have potential antioxdant activities.

발효더덕의 기능성식품 및 화장품 소재로서의 이용 가능성을 조사하기 위해 발효더덕의 열수 추출물의 총 페놀 함량, 총 플라보노이드 함량, 전자공여능, SOD 유사활성, 아질산염 소거능, 환원력을 측정하였다. 발효더덕 추출물의 총 페놀 함량은 2.79 mg/100 g, 총 플라보노이드 함량은 6.19mg/100 g으로 생더덕보다는 총 플라보노이드 및 총 페놀화합물이 많이 함유한 것으로 나타났다. 전자공여능은 발효더덕 추출물에서 32.9$\sim$74.9%, 생더덕에서 8.0$\sim$17.9%의 범위로 발효더덕 추출물에서 비교적 높은 활성을 나타내었다. SOD 유사활성을 측정한 결과 생더덕 추출물은 0.6$\sim$16.5%, 발효더덕 추출물은 3.5$\sim$21.6%의 범위로 분석되었으며, 농도에 따른 유의적 증가 현상을 나타내었다. 아질산염 소거능은 모든 pH 조건(pH 1.2, 3.0, 4.2, 6.0) 하에서 발효더덕 추출물이 높은 아질산염 소거능을 나타내었으며, pH가 증가할수록 감소하였다. 환원력의 경우에는 발효더덕 추출물이 1,000 $\mu g$/mL에서 0.65의 값으로 가장 높은 활성을 보였다. 이상의 결과로부터 발효더덕 추출물은 항산화능이 있음을 확인할 수 있었으며, 발효더덕의 이러한 우수한 결과의 확인을 위하여 발효에 의한 성분 변화에 대하여 더욱 연구가 필요할 것으로 사료된다.

Keywords

References

  1. Kim JP, Chon IJ, Cho HK, Han IH, Whang WK. 2004. The antioxidant and the antidiabetic effects of ethanol extract from biofunctional foods prescriptions. Kor J Pharmacogn 35: 98-103
  2. Dean RT, Gieseg Davies MJ. 1993. Reactive species and their accumulation on radical damaged proteins. Trends Biochem Sci 18: 437-441 https://doi.org/10.1016/0968-0004(93)90145-D
  3. Jung SJ, Lee JH, Song HN, Seong NS, Lee SE, Beak NI. 2004. Screening for antioxidant activity of plant medicinal extracts. J Korean Soc Appl Biol Chem 47: 135-140
  4. Ames BN, Saul RL. 1987. Oxidative DNA damage, cancer and aging. Oxygen and human disease. Am Inter Med 107: 536-539
  5. Branen AL. 1975. Toxicology and biochemistry of butylated hydroxy anisol and butylated hydroxytoluene. J Am Oil Chem Soc 52: 59-63 https://doi.org/10.1007/BF02901825
  6. Choe SY, Yang KH. 1982. Toxicological studies of antioxidants butylated hydroxytoluene (BHT) and butylated hydroxy anisol (BHA). Korean J Food Sci Technol 14: 283-288
  7. Pratt DE, Huang MT, Ho ST, Lee CY. 1992. Phenolic compounds in food and their effects on health (II). In Antioxidants and Cancer Prevention. American Chemical Society, Washington, DC, USA. p 54-71
  8. 임기홍. 1965. 약용식물학(각론). 서울, 동명사. p 354
  9. Kim CH, Chung MH. 1975. Pharmacognostical studies on Condopsis lanceolata. Natural Product Sciences 6: 43-47
  10. Maeng YS, Park HK. 1991. Antioxidant activity of ethanol extract from Dodok (Codonopsis lanceolata). Korean J Food Sci Technol 23: 311-316
  11. Kim SY, Kim HS, Su IS, Yi HS, Kim HS, Chung SY. 1993. Effects of the feeding Platycodon grandiflorum and Codonopsis lanceolata on the lipid components of serum and liver in rats. J Korean Soc Food Nutr 22: 517-523
  12. Han EG, Sung IS, Moon HG, Cho SY. 1998. Effect of Codonopsis lanceolata water extract on the levels of lipid in rats fed high fat diet. J Korean Soc Food Sci Nutr 27: 940-944
  13. Park SJ, Seong DH, Park DS, Kim SS, Gou JY, Ahn JH, Yoon WB, Lee HY. 2009. Chemical compositions of fermented Codonopsis lanceolata. J Korean Soc Food Sci Nutr 38: 396-400 https://doi.org/10.3746/jkfn.2009.38.3.396
  14. Park CK, Tu Q, Yu KW, Jeong JH, Jeong HS, Lee HY. 2009. Chemical composition and antioxidant activity of the fermented Korean ginseng (Panax ginseng C.A. Meyer) with mushroom mycelium by solid culture. Abstract No Ⅱ-23 presented at 2009 Annual Meeting of the Korean Society of Medical Crop Science. Korea
  15. Gutfinger T. 1981. Polyphenols in olive oils. JAOCS 58: 966-967 https://doi.org/10.1007/BF02659771
  16. Moreno MIN, Isla MIN, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical scavenging activity of propolis from several region of Argentiana. J Enthropharmacol 71: 109-114 https://doi.org/10.1016/S0378-8741(99)00189-0
  17. Lee HH, Lee SY. 2008. Cytotoxic and antioxidant effects of Taraxacum coreanum Nakai. and T. officinale WEB. extracts. Korean J Medicinal Crop Sci 16: 79-85
  18. Marklund S, Marklund G. 1975. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  19. Gray JI, Dugan Jr LR. 1975. Inhibition of n-nitrosamine formation in model food system. J Food Sci 40: 981-984 https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  20. Oyaizu M. 1986. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. J apanese J Nutr 44: 307-315 https://doi.org/10.1079/BJN19800045
  21. Kim SH. 2009. Cholesterol lowering activities of Codonopsis lanceolata and Platycodon grandiflorum extracts. PhD Dissertation. Kangwon National University, Chuncheon. p 29-30
  22. Duval B, Shetty K. 2001. The stimulation of phenolics and antioxidant activity in pea (Pisum sativum) elicited by genetically transformed anise root extract. J Food Biochem 25: 361-377 https://doi.org/10.1111/j.1745-4514.2001.tb00746.x
  23. Choi KS, Lee HY. 1999. Characteristics of useful components in the leaves of Baechohyang (Agastache rugosa O. Kuntze). J Korean Soc Food Sci Nutr 28: 326-332
  24. Middleton EJ, Kandaswami C. 1994. Potential health promoting properties of citrus flavonoids. Food Technol 48:115
  25. Na GM, Han HS, Te Sh, Kim HK. 2004. Extraction characteristics and in calcium related disorders of vegetable crops with particular reference to lettuce tipburn, Commun. In Soil Sci Plant Anal 10: 481-485 https://doi.org/10.1080/00103627909366910
  26. Kang YH, Park YK, Lee GD. 1996. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 28: 624-630
  27. Kitani K, Minami C, Amamoto T, Kanai S, Ivy GO, Carrillo MC. 2002. Pharmacological interventions in aging and age-associated disorders: potentials of propargylamines for human use. Ann N Y Acad Sci 959: 295-307 https://doi.org/10.1111/j.1749-6632.2002.tb02101.x
  28. Lim JA, Na YS, Baek SH. 2004. Antioxidative activity and nitrite scavenging ability of ethanol extract from Phyllostachys bambusoides. Korean J Food Sci Technol 36: 306-310
  29. Choi CS, Song ES, Kim JS, Kang MH. 2003. Antioxidative activities of Castanea crenata Flos. methanol extracts. Korean J Food Sci Technol 35: 1216-1220
  30. Chung IM, Kim KH, Ahn JK. 1998. Screening of Korean medicinal and food plants with antioxidant activity. Korean J Medicinal Crop Sci 6: 311-322
  31. Shin JH, Lee JY, Cho HS, Lee SJ, Jung KH, Sung NJ. 2004. Screening of effective factor to inhibition of NDMA formation in Yuza (Citrus junos). J Fd Hyg Safety 19: 126-131
  32. Hong TG, Lee YR, Hyun CN, Yim MH. 2004. Physiological functionality and nitrite scavenging ability of fermentation extracts from pine needles. Korean J Food Preserv 11: 94-99
  33. Park CS, Kwon CJ, Choi MA, Park GS, Choi KH. 2002. Antioxidative and nitrite scavenging activities of mugwort and pine needle extracts. Korean J Food Preserv 9: 248-252
  34. Kim JH, Kim JK, Kang WW, Ha YS, Choi SW, Moon KD. 2003. Chemical composition and DPPH radical scavenger activity in different sections of safflower. J Korean Soc Food Sci Nutr 32: 733-738 https://doi.org/10.3746/jkfn.2003.32.5.733

Cited by

  1. Increasement of antioxidative activity in Codonopsis lanceolata adventitious root treated by Methyl jasmonate and salicylic acid vol.40, pp.3, 2013, https://doi.org/10.5010/JPB.2013.40.3.178
  2. The Effect of Fermented Codonopsis lanceolata on the Memory Impairment of Mice vol.39, pp.11, 2010, https://doi.org/10.3746/jkfn.2010.39.11.1691
  3. Comparison on Cosmetic Activities of Acer mono Bark and Sap vol.19, pp.4, 2011, https://doi.org/10.7783/KJMCS.2011.19.4.264
  4. Change of Total Polyphenol Content of Fermented Gastrodia elata Blume and Radical Scavenging vol.25, pp.4, 2012, https://doi.org/10.7732/kjpr.2012.25.4.379
  5. Antioxidative and Anticancer Activities of Water Extracts from Different Parts of Taraxacum coreanum Nakai Cultivated in Korea vol.44, pp.8, 2015, https://doi.org/10.3746/jkfn.2015.44.8.1234
  6. Physicochemical components and antioxidant activity of Sparassis crispa mixture fermented by lactic acid bacteria vol.23, pp.3, 2016, https://doi.org/10.11002/kjfp.2016.23.3.361
  7. Enhancement of Antioxidant Activities of Codonopsis lanceolata and Fermented Codonopsis lanceolata by Ultra High Pressure Extraction vol.39, pp.12, 2010, https://doi.org/10.3746/jkfn.2010.39.12.1898
  8. Physicochemical Properties and Antioxidative Activity of Lactic Acid Bacteria Fermented Rhodiola sachalinensis using Adsorption Process vol.25, pp.4, 2012, https://doi.org/10.9799/ksfan.2012.25.4.779
  9. Antioxidant Activities of Processed Deoduck (Codonopsis lanceolata) Extracts vol.42, pp.6, 2013, https://doi.org/10.3746/jkfn.2013.42.6.924
  10. Development of Functional Food Materials from Acanthopanax senticosus-Fermented Mushroom Mycelia vol.43, pp.3, 2014, https://doi.org/10.3746/jkfn.2014.43.3.411
  11. Biological Activities of Hominis Placenta Herbal Acupuncture prepared by Hydrochloric Acid Hydrolysis vol.13, pp.2, 2010, https://doi.org/10.3831/KPI.2010.13.2.005
  12. Biological Activity and Chemical Characteristics of Fermented Acanthopanax senticosus by Mold vol.22, pp.12, 2012, https://doi.org/10.5352/JLS.2012.22.12.1704
  13. Antioxidant Activity and α-Glucosidase Inhibitory Effect of Jerusalem Artichoke (Helianthus tuberosus) Methanol Extracts by Heat Treatment Conditions vol.19, pp.4, 2011, https://doi.org/10.7783/KJMCS.2011.19.4.257
  14. Biological Activity and Biochemical Properties of Silkworm (Bombyx mori L.) Powder Fermented with Bacillus subtilis and Aspergillus kawachii vol.21, pp.1, 2011, https://doi.org/10.5352/JLS.2011.21.1.81
  15. Ingredients Analysis and Biological Activity of Fermented Angelica gigas Nakai by Mold vol.20, pp.9, 2010, https://doi.org/10.5352/JLS.2010.20.9.1385
  16. Antioxidative Activity and Cytotoxicity of Fermented Allium victorialis L. Extract vol.24, pp.1, 2011, https://doi.org/10.7732/kjpr.2011.24.1.030
  17. Antioxidative Activities of Water Extracts from Different Parts of Taraxacum officinale vol.39, pp.11, 2010, https://doi.org/10.3746/jkfn.2010.39.11.1580
  18. Biological Activities of Scolopendrid Pharmacopuncture vol.13, pp.3, 2010, https://doi.org/10.3831/KPI.2010.13.3.005
  19. Antioxidative Effect of Seven Fermented Medicinal Herb Mixtures Using in Vitro Assays and Bulk Oil System vol.34, pp.4, 2018, https://doi.org/10.9724/kfcs.2018.34.4.342
  20. 자생 돌더덕 추출물에 의한 천식억제 활성의 분석 vol.27, pp.4, 2017, https://doi.org/10.5352/jls.2017.27.4.450
  21. 한국산 초롱꽃과(Campanulaceae)의 민속식물, 화학성분, 약리작용에 대한 종합적 고찰 vol.30, pp.2, 2009, https://doi.org/10.7732/kjpr.2017.30.2.240
  22. 참죽의 추출방법에 따른 수용성 다당류의 생리기능성 vol.49, pp.2, 2009, https://doi.org/10.9721/kjfst.2017.49.2.215
  23. 아이스플랜트(Mesembryanthemum crystallinum L.) 발효추출물의 항산화, 항당뇨 및 간 보호효과 vol.27, pp.8, 2017, https://doi.org/10.5352/jls.2017.27.8.909
  24. 국내산 더덕의 Tangshenoside I과 Lobetyolin 정량분석 vol.31, pp.6, 2009, https://doi.org/10.9799/ksfan.2018.31.6.957
  25. 다시마 물 추출액과 발효액의 항산화 및 항염증 활성 vol.29, pp.5, 2009, https://doi.org/10.5352/jls.2019.29.5.596
  26. 더덕 에탄올 추출물의 도시미세먼지 노출로 인한 폐 세포 산화스트레스 발생과 세포밀착연접 손상 억제 효과 vol.53, pp.2, 2009, https://doi.org/10.9721/kjfst.2021.53.2.165